“Do It Yourself” Microbial Cultivation Techniques for Synthetic and Systems Biology: Cheap, Fun, and Flexible

• Main Authors: Teuta Pilizota, Ya-Tang Yang
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2018-07-01
• Series: Frontiers in Microbiology
• Subjects: bioreactor; synthetic biology; systems biology; microbial cultivation; evolution; optogenetics
• Online Access: https://www.frontiersin.org/article/10.3389/fmicb.2018.01666/full

With the emergence of inexpensive 3D printing technology, open-source platforms for electronic prototyping and single-board computers, “Do it Yourself” (DIY) approaches to the cultivation of microbial cultures are becoming more feasible, user-friendly, and thus wider spread. In this perspective, we survey some of these approaches, as well as add-on solutions to commercial instruments for synthetic and system biology applications. We discuss different cultivation designs, including capabilities and limitations. Our intention is to encourage the reader to consider the DIY solutions. Overall, custom cultivation devices offer controlled growth environments with in-line monitoring of, for example, optical density, fluorescence, pH, and dissolved oxygen, all at affordable prices. Moreover, they offer a great degree of flexibility for different applications and requirements and are fun to design and construct. We include several illustrative examples, such as gaining optogenetic control and adaptive laboratory evolution experiments.