Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex
Abstract Background Supplementation of bovine oocyte-cumulus complexes during in vitro maturation (IVM) with 1 μM of docosahexaenoic acid (DHA), C22:6 n-3 polyunsaturated fatty acid, was reported to improve in vitro embryo development. The objective of this paper was to decipher the mechanisms of DH...
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doaj-054de7ab1ff146c7a888b53150c011ab2020-11-25T02:42:07ZengBMCJournal of Ovarian Research1757-22152017-11-0110111410.1186/s13048-017-0370-zDocosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complexSebastien Elis0Mouhamad Oseikria1Anais Vitorino Carvalho2Priscila Silvana Bertevello3Emilie Corbin4Ana-Paula Teixeira-Gomes5Jérôme Lecardonnel6Catherine Archilla7Véronique Duranthon8Valérie Labas9Svetlana Uzbekova10UMR PRC, CNRS, IFCE, INRA, Université de ToursUMR PRC, CNRS, IFCE, INRA, Université de ToursUMR PRC, CNRS, IFCE, INRA, Université de ToursUMR PRC, CNRS, IFCE, INRA, Université de ToursUMR PRC, CNRS, IFCE, INRA, Université de ToursUMR ISP, INRA, Université de ToursGABI, INRA, Agroparis Tech, Université de Paris-SaclayUMR BDR, ENVA, INRA, Université Paris-SaclayUMR BDR, ENVA, INRA, Université Paris-SaclayUMR PRC, CNRS, IFCE, INRA, Université de ToursUMR PRC, CNRS, IFCE, INRA, Université de ToursAbstract Background Supplementation of bovine oocyte-cumulus complexes during in vitro maturation (IVM) with 1 μM of docosahexaenoic acid (DHA), C22:6 n-3 polyunsaturated fatty acid, was reported to improve in vitro embryo development. The objective of this paper was to decipher the mechanisms of DHA action. Results Transcriptomic analysis of 1 μM DHA-treated and control cumulus cells after 4 h IVM showed no significant difference in gene expression. MALDI-TOF mass spectrometry analysis of lipid profiles in DHA-treated and control oocytes and cumulus cells after IVM showed variations of only 3 out of 700 molecular species in oocytes and 7 out of 698 species in cumulus cells (p < 0.01). We showed expression of free fatty acid receptor FFAR4 in both oocytes and cumulus cells, this receptor is known to be activated by binding to DHA. FFAR4 protein was localized close to the cellular membrane by immunofluorescence. Functional studies demonstrated that supplementation with FFAR4 agonist TUG-891 (1 μM or 5 μM) during IVM led to an increased blastocyst rate (39.5% ± 4.1%, 41.3% ± 4.1%), similar to DHA 1 μM treatment (39.2% ± 4.1%) as compared to control (25.2% ± 3.6%). FFAR4 activation via TUG-891 led to beneficial effect on oocyte developmental competence and might explain in part similar effects of DHA. Conclusions In conclusion, we suggested that low dose of DHA (1 μM) during IVM might activate regulatory mechanisms without evident effect on gene expression and lipid content in oocyte-cumulus complexes, likely through signaling pathways which need to be elucidated in further studies.http://link.springer.com/article/10.1186/s13048-017-0370-zBovineOocyte-cumulus complexFFAR4DHAOocyte maturation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sebastien Elis Mouhamad Oseikria Anais Vitorino Carvalho Priscila Silvana Bertevello Emilie Corbin Ana-Paula Teixeira-Gomes Jérôme Lecardonnel Catherine Archilla Véronique Duranthon Valérie Labas Svetlana Uzbekova |
spellingShingle |
Sebastien Elis Mouhamad Oseikria Anais Vitorino Carvalho Priscila Silvana Bertevello Emilie Corbin Ana-Paula Teixeira-Gomes Jérôme Lecardonnel Catherine Archilla Véronique Duranthon Valérie Labas Svetlana Uzbekova Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex Journal of Ovarian Research Bovine Oocyte-cumulus complex FFAR4 DHA Oocyte maturation |
author_facet |
Sebastien Elis Mouhamad Oseikria Anais Vitorino Carvalho Priscila Silvana Bertevello Emilie Corbin Ana-Paula Teixeira-Gomes Jérôme Lecardonnel Catherine Archilla Véronique Duranthon Valérie Labas Svetlana Uzbekova |
author_sort |
Sebastien Elis |
title |
Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex |
title_short |
Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex |
title_full |
Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex |
title_fullStr |
Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex |
title_full_unstemmed |
Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex |
title_sort |
docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex |
publisher |
BMC |
series |
Journal of Ovarian Research |
issn |
1757-2215 |
publishDate |
2017-11-01 |
description |
Abstract Background Supplementation of bovine oocyte-cumulus complexes during in vitro maturation (IVM) with 1 μM of docosahexaenoic acid (DHA), C22:6 n-3 polyunsaturated fatty acid, was reported to improve in vitro embryo development. The objective of this paper was to decipher the mechanisms of DHA action. Results Transcriptomic analysis of 1 μM DHA-treated and control cumulus cells after 4 h IVM showed no significant difference in gene expression. MALDI-TOF mass spectrometry analysis of lipid profiles in DHA-treated and control oocytes and cumulus cells after IVM showed variations of only 3 out of 700 molecular species in oocytes and 7 out of 698 species in cumulus cells (p < 0.01). We showed expression of free fatty acid receptor FFAR4 in both oocytes and cumulus cells, this receptor is known to be activated by binding to DHA. FFAR4 protein was localized close to the cellular membrane by immunofluorescence. Functional studies demonstrated that supplementation with FFAR4 agonist TUG-891 (1 μM or 5 μM) during IVM led to an increased blastocyst rate (39.5% ± 4.1%, 41.3% ± 4.1%), similar to DHA 1 μM treatment (39.2% ± 4.1%) as compared to control (25.2% ± 3.6%). FFAR4 activation via TUG-891 led to beneficial effect on oocyte developmental competence and might explain in part similar effects of DHA. Conclusions In conclusion, we suggested that low dose of DHA (1 μM) during IVM might activate regulatory mechanisms without evident effect on gene expression and lipid content in oocyte-cumulus complexes, likely through signaling pathways which need to be elucidated in further studies. |
topic |
Bovine Oocyte-cumulus complex FFAR4 DHA Oocyte maturation |
url |
http://link.springer.com/article/10.1186/s13048-017-0370-z |
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