Nobiletin Delays Aging and Enhances Stress Resistance of <i>Caenorhabditis elegans</i>
Nobiletin (NOB), one of polymethoxyflavone existing in citrus fruits, has been reported to exhibit a multitude of biological properties, including anti-inflammation, anti-oxidation, anti-atherosclerosis, neuroprotection, and anti-tumor activity. However, little is known about the anti-aging effect o...
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doaj-056a4dd7720e40b5ab27b538fefa02772020-11-25T02:41:18ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-01-0121134110.3390/ijms21010341ijms21010341Nobiletin Delays Aging and Enhances Stress Resistance of <i>Caenorhabditis elegans</i>Xueyan Yang0Hong Wang1Tong Li2Ling Chen3Bisheng Zheng4Rui Hai Liu5Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, ChinaOverseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, ChinaDepartment of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853, USAMinistry of Education Engineering Research Centre of Starch & Protein Processing, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510641, ChinaOverseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, ChinaDepartment of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853, USANobiletin (NOB), one of polymethoxyflavone existing in citrus fruits, has been reported to exhibit a multitude of biological properties, including anti-inflammation, anti-oxidation, anti-atherosclerosis, neuroprotection, and anti-tumor activity. However, little is known about the anti-aging effect of NOB. The objective of this study was to determine the effects of NOB on lifespan, stress resistance, and its associated gene expression. Using <i>Caenorhabditis elegans</i>, an in vivo nematode model, we found that NOB remarkably extended the lifespan; slowed aging-related functional declines; and increased the resistance against various stressors, including heat shock and ultraviolet radiation. Also, NOB reduced the effects of paraquat stressor on nematodes and scavenged reactive oxygen species (ROS). Furthermore, gene expression revealed that NOB upregulated the expression of <i>sod-3</i>, <i>hsp-16.2</i>, <i>gst-4</i>, <i>skn-1</i>, <i>sek-1</i>, and <i>sir-2.1</i>, which was suggested that anti-aging activity of NOB was mediated most likely by activation of the target genes of the transcription factors including dauer formation (DAF)-16, heat-shock transcription factor (HSF)-1, and skinhead (SKN)-1. In summary, NOB has potential application in extension of lifespan, and its associated healthspan and stress resistances.https://www.mdpi.com/1422-0067/21/1/341nobiletinagingstress resistance<i>caenorhabditis elegans</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xueyan Yang Hong Wang Tong Li Ling Chen Bisheng Zheng Rui Hai Liu |
spellingShingle |
Xueyan Yang Hong Wang Tong Li Ling Chen Bisheng Zheng Rui Hai Liu Nobiletin Delays Aging and Enhances Stress Resistance of <i>Caenorhabditis elegans</i> International Journal of Molecular Sciences nobiletin aging stress resistance <i>caenorhabditis elegans</i> |
author_facet |
Xueyan Yang Hong Wang Tong Li Ling Chen Bisheng Zheng Rui Hai Liu |
author_sort |
Xueyan Yang |
title |
Nobiletin Delays Aging and Enhances Stress Resistance of <i>Caenorhabditis elegans</i> |
title_short |
Nobiletin Delays Aging and Enhances Stress Resistance of <i>Caenorhabditis elegans</i> |
title_full |
Nobiletin Delays Aging and Enhances Stress Resistance of <i>Caenorhabditis elegans</i> |
title_fullStr |
Nobiletin Delays Aging and Enhances Stress Resistance of <i>Caenorhabditis elegans</i> |
title_full_unstemmed |
Nobiletin Delays Aging and Enhances Stress Resistance of <i>Caenorhabditis elegans</i> |
title_sort |
nobiletin delays aging and enhances stress resistance of <i>caenorhabditis elegans</i> |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-01-01 |
description |
Nobiletin (NOB), one of polymethoxyflavone existing in citrus fruits, has been reported to exhibit a multitude of biological properties, including anti-inflammation, anti-oxidation, anti-atherosclerosis, neuroprotection, and anti-tumor activity. However, little is known about the anti-aging effect of NOB. The objective of this study was to determine the effects of NOB on lifespan, stress resistance, and its associated gene expression. Using <i>Caenorhabditis elegans</i>, an in vivo nematode model, we found that NOB remarkably extended the lifespan; slowed aging-related functional declines; and increased the resistance against various stressors, including heat shock and ultraviolet radiation. Also, NOB reduced the effects of paraquat stressor on nematodes and scavenged reactive oxygen species (ROS). Furthermore, gene expression revealed that NOB upregulated the expression of <i>sod-3</i>, <i>hsp-16.2</i>, <i>gst-4</i>, <i>skn-1</i>, <i>sek-1</i>, and <i>sir-2.1</i>, which was suggested that anti-aging activity of NOB was mediated most likely by activation of the target genes of the transcription factors including dauer formation (DAF)-16, heat-shock transcription factor (HSF)-1, and skinhead (SKN)-1. In summary, NOB has potential application in extension of lifespan, and its associated healthspan and stress resistances. |
topic |
nobiletin aging stress resistance <i>caenorhabditis elegans</i> |
url |
https://www.mdpi.com/1422-0067/21/1/341 |
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