Longitudinal assessment of antibiotic resistance gene profiles in gut microbiomes of infants at risk of eczema
Abstract Background While there is increasing knowledge about the gut microbiome, the factors influencing and the significance of the gut resistome are still not well understood. Infant gut commensals risk transferring multidrug-resistant antibiotic resistance genes (ARGs) to pathogenic bacteria. Th...
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2020-04-01
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Online Access: | http://link.springer.com/article/10.1186/s12879-020-05000-y |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Evelyn Xiu Ling Loo Amanda Zain Gaik Chin Yap Rikky W. Purbojati Daniela I. Drautz-Moses Yan Qing Koh Yap Seng Chong Kok Hian Tan Peter D. Gluckman Fabian Yap Johan Gunnar Eriksson Elizabeth Tham Lynette Pei-chi Shek Staffan Kjelleberg Stephan C. Schuster Ritu Banerjee Bee Wah Lee |
spellingShingle |
Evelyn Xiu Ling Loo Amanda Zain Gaik Chin Yap Rikky W. Purbojati Daniela I. Drautz-Moses Yan Qing Koh Yap Seng Chong Kok Hian Tan Peter D. Gluckman Fabian Yap Johan Gunnar Eriksson Elizabeth Tham Lynette Pei-chi Shek Staffan Kjelleberg Stephan C. Schuster Ritu Banerjee Bee Wah Lee Longitudinal assessment of antibiotic resistance gene profiles in gut microbiomes of infants at risk of eczema BMC Infectious Diseases Antibiotic resistance genes Resistome Infancy Birth cohort ESBL producing Enterobacteriaceae |
author_facet |
Evelyn Xiu Ling Loo Amanda Zain Gaik Chin Yap Rikky W. Purbojati Daniela I. Drautz-Moses Yan Qing Koh Yap Seng Chong Kok Hian Tan Peter D. Gluckman Fabian Yap Johan Gunnar Eriksson Elizabeth Tham Lynette Pei-chi Shek Staffan Kjelleberg Stephan C. Schuster Ritu Banerjee Bee Wah Lee |
author_sort |
Evelyn Xiu Ling Loo |
title |
Longitudinal assessment of antibiotic resistance gene profiles in gut microbiomes of infants at risk of eczema |
title_short |
Longitudinal assessment of antibiotic resistance gene profiles in gut microbiomes of infants at risk of eczema |
title_full |
Longitudinal assessment of antibiotic resistance gene profiles in gut microbiomes of infants at risk of eczema |
title_fullStr |
Longitudinal assessment of antibiotic resistance gene profiles in gut microbiomes of infants at risk of eczema |
title_full_unstemmed |
Longitudinal assessment of antibiotic resistance gene profiles in gut microbiomes of infants at risk of eczema |
title_sort |
longitudinal assessment of antibiotic resistance gene profiles in gut microbiomes of infants at risk of eczema |
publisher |
BMC |
series |
BMC Infectious Diseases |
issn |
1471-2334 |
publishDate |
2020-04-01 |
description |
Abstract Background While there is increasing knowledge about the gut microbiome, the factors influencing and the significance of the gut resistome are still not well understood. Infant gut commensals risk transferring multidrug-resistant antibiotic resistance genes (ARGs) to pathogenic bacteria. The rapid spread of multidrug-resistant pathogenic bacteria is a worldwide public health concern. Better understanding of the naïve infant gut resistome may build the evidence base for antimicrobial stewardship in both humans and in the food industry. Given the high carriage rate of extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in Asia, we aimed to evaluate community prevalence, dynamics, and longitudinal changes in antibiotic resistance gene (ARG) profiles and prevalence of ESBL-producing E. coli and K. pneumoniae in the intestinal microbiome of infants participating in the Growing Up in Singapore Towards Healthy Outcomes (GUSTO) study, a longitudinal cohort study of pregnant women and their infants. Methods We analysed ARGs in the first year of life among 75 infants at risk of eczema who had stool samples collected at multiple timepoints using metagenomics. Results The mean number of ARGs per infant increased with age. The most common ARGs identified confer resistance to aminoglycoside, beta-lactam, macrolide and tetracycline antibiotics; all infants harboured these antibiotic resistance genes at some point in the first year of life. Few ARGs persisted throughout the first year of life. Beta-lactam resistant Escherichia coli and Klebsiella pneumoniae were detected in 4 (5.3%) and 32 (42.7%) of subjects respectively. Conclusion In this longitudinal cohort study of infants living in a region with high endemic antibacterial resistance, we demonstrate that majority of the infants harboured several antibiotic resistance genes in their gut and showed that the infant gut resistome is diverse and dynamic over the first year of life. |
topic |
Antibiotic resistance genes Resistome Infancy Birth cohort ESBL producing Enterobacteriaceae |
url |
http://link.springer.com/article/10.1186/s12879-020-05000-y |
work_keys_str_mv |
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doaj-05747fd921eb46579167c795e5d7e90d2020-11-25T03:47:14ZengBMCBMC Infectious Diseases1471-23342020-04-0120111210.1186/s12879-020-05000-yLongitudinal assessment of antibiotic resistance gene profiles in gut microbiomes of infants at risk of eczemaEvelyn Xiu Ling Loo0Amanda Zain1Gaik Chin Yap2Rikky W. Purbojati3Daniela I. Drautz-Moses4Yan Qing Koh5Yap Seng Chong6Kok Hian Tan7Peter D. Gluckman8Fabian Yap9Johan Gunnar Eriksson10Elizabeth Tham11Lynette Pei-chi Shek12Staffan Kjelleberg13Stephan C. Schuster14Ritu Banerjee15Bee Wah Lee16Singapore Institute for Clinical Sciences (SICS), Agency for Science, Technology and Research (A*STAR)Department of Paediatrics, Yong Loo Lin School of Medicine, National University of SingaporeDepartment of Paediatrics, Yong Loo Lin School of Medicine, National University of SingaporeSingapore Centre For Environmental Life Sciences Engineering (SCELSE), Nanyang Technological UniversitySingapore Centre For Environmental Life Sciences Engineering (SCELSE), Nanyang Technological UniversitySingapore Centre For Environmental Life Sciences Engineering (SCELSE), Nanyang Technological UniversitySingapore Institute for Clinical Sciences (SICS), Agency for Science, Technology and Research (A*STAR)Department of Maternal Fetal Medicine, KK Women’s and Children’s Hospital (KKH)Singapore Institute for Clinical Sciences (SICS), Agency for Science, Technology and Research (A*STAR)Department of Endocrinology KK Women’s and Children’s Hospital (KKH)Singapore Institute for Clinical Sciences (SICS), Agency for Science, Technology and Research (A*STAR)Department of Paediatrics, Yong Loo Lin School of Medicine, National University of SingaporeSingapore Institute for Clinical Sciences (SICS), Agency for Science, Technology and Research (A*STAR)Singapore Centre For Environmental Life Sciences Engineering (SCELSE), Nanyang Technological UniversitySingapore Centre For Environmental Life Sciences Engineering (SCELSE), Nanyang Technological UniversityVanderbilt University Medical CenterDepartment of Paediatrics, Yong Loo Lin School of Medicine, National University of SingaporeAbstract Background While there is increasing knowledge about the gut microbiome, the factors influencing and the significance of the gut resistome are still not well understood. Infant gut commensals risk transferring multidrug-resistant antibiotic resistance genes (ARGs) to pathogenic bacteria. The rapid spread of multidrug-resistant pathogenic bacteria is a worldwide public health concern. Better understanding of the naïve infant gut resistome may build the evidence base for antimicrobial stewardship in both humans and in the food industry. Given the high carriage rate of extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in Asia, we aimed to evaluate community prevalence, dynamics, and longitudinal changes in antibiotic resistance gene (ARG) profiles and prevalence of ESBL-producing E. coli and K. pneumoniae in the intestinal microbiome of infants participating in the Growing Up in Singapore Towards Healthy Outcomes (GUSTO) study, a longitudinal cohort study of pregnant women and their infants. Methods We analysed ARGs in the first year of life among 75 infants at risk of eczema who had stool samples collected at multiple timepoints using metagenomics. Results The mean number of ARGs per infant increased with age. The most common ARGs identified confer resistance to aminoglycoside, beta-lactam, macrolide and tetracycline antibiotics; all infants harboured these antibiotic resistance genes at some point in the first year of life. Few ARGs persisted throughout the first year of life. Beta-lactam resistant Escherichia coli and Klebsiella pneumoniae were detected in 4 (5.3%) and 32 (42.7%) of subjects respectively. Conclusion In this longitudinal cohort study of infants living in a region with high endemic antibacterial resistance, we demonstrate that majority of the infants harboured several antibiotic resistance genes in their gut and showed that the infant gut resistome is diverse and dynamic over the first year of life.http://link.springer.com/article/10.1186/s12879-020-05000-yAntibiotic resistance genesResistomeInfancyBirth cohortESBL producing Enterobacteriaceae |