Decitabine Inhibits Bone Resorption in Periodontitis by Upregulating Anti-Inflammatory Cytokines and Suppressing Osteoclastogenesis

• Main Authors: Urara Tanaka, Shunichi Kajioka, Livia S. Finoti, Daniela B. Palioto, Denis F. Kinane, Manjunatha R. Benakanakere
• Format: Article
• Language: English
• Published: MDPI AG 2021-02-01
• Series: Biomedicines
• Subjects: periodontitis; periodontal bone-loss; decitabine; Krüppel-like transcription factor-2; interleukin-10; transforming growth factor beta-1
• Online Access: https://www.mdpi.com/2227-9059/9/2/199

DNA methylation controls several inflammatory genes affecting bone homeostasis. Hitherto, inhibition of DNA methylation in vivo in the context of periodontitis and osteoclastogenesis has not been attempted. Ligature-induced periodontitis in C57BL/6J mice was induced by placing ligature for five days with Decitabine (5-aza-2′-deoxycytidine) (1 mg/kg/day) or vehicle treatment. We evaluated bone resorption, osteoclast differentiation by tartrate-resistant acid phosphatase (TRAP) and mRNA expression of anti-inflammatory molecules using cluster differentiation 14 positive (CD14⁺) monocytes from human peripheral blood. Our data showed that decitabine inhibited bone loss and osteoclast differentiation experimental periodontitis, and suppressed osteoclast CD14⁺ human monocytes; and conversely, that it increased bone mineralization in osteoblastic cell line MC3T3-E1 in a concentration-dependent manner. In addition to increasing <i>IL10</i> (interleukin-10), <i>TGFB</i> (transforming growth factor beta-1) in CD14⁺ monocytes, decitabine upregulated KLF2 (Krüppel-like factor-2) expression. Overexpression of KLF2 protein enhanced the transcription of <i>IL10</i> and <i>TGFB.</i> On the contrary, site-directed mutagenesis of <i>KLF2</i> binding site in <i>IL10</i> and <i>TFGB</i> abrogated luciferase activity in HEK293T cells. Decitabine reduces bone loss in a mouse model of periodontitis by inhibiting osteoclastogenesis through the upregulation of anti-inflammatory cytokines via KLF2 dependent mechanisms. DNA methyltransferase inhibitors merit further investigation as a possible novel therapy for periodontitis.