An Origami Paper-Based Device Printed with DNAzyme-Containing DNA Superstructures for <em>Escherichia coli</em> Detection
Rapid detection of pathogenic bacteria is extremely important for public health and safety. Here, we describe for the first time an integrated origami paper-based analytical device (PAD) incorporating cell lysis, molecular recognition, amplification and visual detection of <i>Escherichia coli&...
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MDPI AG
2019-08-01
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doaj-059952051ef847599cf93d1e14832c712020-11-25T00:28:02ZengMDPI AGMicromachines2072-666X2019-08-0110853110.3390/mi10080531mi10080531An Origami Paper-Based Device Printed with DNAzyme-Containing DNA Superstructures for <em>Escherichia coli</em> DetectionYating Sun0Yangyang Chang1Qiang Zhang2Meng Liu3School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian 116024, ChinaSchool of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian 116024, ChinaSchool of Bioengineering, Dalian University of Technology, Dalian 116024, ChinaSchool of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian 116024, ChinaRapid detection of pathogenic bacteria is extremely important for public health and safety. Here, we describe for the first time an integrated origami paper-based analytical device (PAD) incorporating cell lysis, molecular recognition, amplification and visual detection of <i>Escherichia coli</i> (<i>E. coli</i>). The device features three components: paper for its ability to extract protein molecules nonspecifically from cells, DNA superstructures for their ability to immobilize RNA-cleaving DNAzymes (RCDs) but undergo target-induced RNA cleavage on paper, and isothermal rolling circle amplification (RCA) for its ability to amplify each cleavage event into repetitive sequence units that can be detected by naked eye. This device can achieve detection of <i>E. coli</i> K12 with a detection limit of as low as 10<sup>3</sup> CFU·mL<sup>−1</sup> in a total turnaround time of 35 min. Furthermore, this device allowed the sensitive detection of <i>E. coli</i> in complex sample matrices such as juice and milk. Given that more specific RCDs can be evolved for diverse bacteria, the integrated PAD holds great potential for rapid, sensitive and highly selective detection of pathogenic bacteria in resource-limited settings.https://www.mdpi.com/2072-666X/10/8/531colorimetricDNAzyme<i>Escherichia coli</i>paper-based devicerolling circle amplification |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yating Sun Yangyang Chang Qiang Zhang Meng Liu |
spellingShingle |
Yating Sun Yangyang Chang Qiang Zhang Meng Liu An Origami Paper-Based Device Printed with DNAzyme-Containing DNA Superstructures for <em>Escherichia coli</em> Detection Micromachines colorimetric DNAzyme <i>Escherichia coli</i> paper-based device rolling circle amplification |
author_facet |
Yating Sun Yangyang Chang Qiang Zhang Meng Liu |
author_sort |
Yating Sun |
title |
An Origami Paper-Based Device Printed with DNAzyme-Containing DNA Superstructures for <em>Escherichia coli</em> Detection |
title_short |
An Origami Paper-Based Device Printed with DNAzyme-Containing DNA Superstructures for <em>Escherichia coli</em> Detection |
title_full |
An Origami Paper-Based Device Printed with DNAzyme-Containing DNA Superstructures for <em>Escherichia coli</em> Detection |
title_fullStr |
An Origami Paper-Based Device Printed with DNAzyme-Containing DNA Superstructures for <em>Escherichia coli</em> Detection |
title_full_unstemmed |
An Origami Paper-Based Device Printed with DNAzyme-Containing DNA Superstructures for <em>Escherichia coli</em> Detection |
title_sort |
origami paper-based device printed with dnazyme-containing dna superstructures for <em>escherichia coli</em> detection |
publisher |
MDPI AG |
series |
Micromachines |
issn |
2072-666X |
publishDate |
2019-08-01 |
description |
Rapid detection of pathogenic bacteria is extremely important for public health and safety. Here, we describe for the first time an integrated origami paper-based analytical device (PAD) incorporating cell lysis, molecular recognition, amplification and visual detection of <i>Escherichia coli</i> (<i>E. coli</i>). The device features three components: paper for its ability to extract protein molecules nonspecifically from cells, DNA superstructures for their ability to immobilize RNA-cleaving DNAzymes (RCDs) but undergo target-induced RNA cleavage on paper, and isothermal rolling circle amplification (RCA) for its ability to amplify each cleavage event into repetitive sequence units that can be detected by naked eye. This device can achieve detection of <i>E. coli</i> K12 with a detection limit of as low as 10<sup>3</sup> CFU·mL<sup>−1</sup> in a total turnaround time of 35 min. Furthermore, this device allowed the sensitive detection of <i>E. coli</i> in complex sample matrices such as juice and milk. Given that more specific RCDs can be evolved for diverse bacteria, the integrated PAD holds great potential for rapid, sensitive and highly selective detection of pathogenic bacteria in resource-limited settings. |
topic |
colorimetric DNAzyme <i>Escherichia coli</i> paper-based device rolling circle amplification |
url |
https://www.mdpi.com/2072-666X/10/8/531 |
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