Single-Cell Technologies Applied to HIV-1 Research: Reaching Maturity
The need for definitive answers probably explains our natural tendency to seek simplicity. The reductionist “bulk” approach, in which a mean behavior is attributed to a heterogeneous cell population, fulfills this need by considerably helping the conceptualization of complex biological processes. Ho...
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doaj-05a8051a85394443afd400b5e282acf02020-11-25T00:37:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-03-011110.3389/fmicb.2020.00297509151Single-Cell Technologies Applied to HIV-1 Research: Reaching MaturityGérémy Sannier0Gérémy Sannier1Mathieu Dubé2Daniel E. Kaufmann3Daniel E. Kaufmann4Daniel E. Kaufmann5Research Centre of the Centre Hospitalier de l’Université de Montréal (CRCHUM), Montreal, QC, CanadaDepartment of Microbiology, Infectiology and Immunology, Université de Montréal, Montreal, QC, CanadaResearch Centre of the Centre Hospitalier de l’Université de Montréal (CRCHUM), Montreal, QC, CanadaResearch Centre of the Centre Hospitalier de l’Université de Montréal (CRCHUM), Montreal, QC, CanadaDepartment of Medicine, Université de Montréal, Montreal, QC, CanadaConsortium for HIV/AIDS Vaccine Development (Scripps CHAVD), La Jolla, CA, United StatesThe need for definitive answers probably explains our natural tendency to seek simplicity. The reductionist “bulk” approach, in which a mean behavior is attributed to a heterogeneous cell population, fulfills this need by considerably helping the conceptualization of complex biological processes. However, the limits of this methodology are becoming increasingly clear as models seek to explain biological events occurring in vivo, where heterogeneity is the rule. Research in the HIV-1 field is no exception: the challenges encountered in the development of preventive and curative anti-HIV-1 strategies may well originate in part from inadequate assumptions built on bulk technologies, highlighting the need for new perspectives. The emergence of diverse single-cell technologies set the stage for potential breakthrough discoveries, as heterogeneous processes can now be investigated with an unprecedented depth in topics as diverse as HIV-1 tropism, dynamics of the replication cycle, latency, viral reservoirs and immune control. In this review, we summarize recent advances in the HIV-1 field made possible by single-cell technologies, and contextualize their importance.https://www.frontiersin.org/article/10.3389/fmicb.2020.00297/fullHIV-1single-cell technologiespathogenesiscurevaccinesingle-cell omics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gérémy Sannier Gérémy Sannier Mathieu Dubé Daniel E. Kaufmann Daniel E. Kaufmann Daniel E. Kaufmann |
spellingShingle |
Gérémy Sannier Gérémy Sannier Mathieu Dubé Daniel E. Kaufmann Daniel E. Kaufmann Daniel E. Kaufmann Single-Cell Technologies Applied to HIV-1 Research: Reaching Maturity Frontiers in Microbiology HIV-1 single-cell technologies pathogenesis cure vaccine single-cell omics |
author_facet |
Gérémy Sannier Gérémy Sannier Mathieu Dubé Daniel E. Kaufmann Daniel E. Kaufmann Daniel E. Kaufmann |
author_sort |
Gérémy Sannier |
title |
Single-Cell Technologies Applied to HIV-1 Research: Reaching Maturity |
title_short |
Single-Cell Technologies Applied to HIV-1 Research: Reaching Maturity |
title_full |
Single-Cell Technologies Applied to HIV-1 Research: Reaching Maturity |
title_fullStr |
Single-Cell Technologies Applied to HIV-1 Research: Reaching Maturity |
title_full_unstemmed |
Single-Cell Technologies Applied to HIV-1 Research: Reaching Maturity |
title_sort |
single-cell technologies applied to hiv-1 research: reaching maturity |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-03-01 |
description |
The need for definitive answers probably explains our natural tendency to seek simplicity. The reductionist “bulk” approach, in which a mean behavior is attributed to a heterogeneous cell population, fulfills this need by considerably helping the conceptualization of complex biological processes. However, the limits of this methodology are becoming increasingly clear as models seek to explain biological events occurring in vivo, where heterogeneity is the rule. Research in the HIV-1 field is no exception: the challenges encountered in the development of preventive and curative anti-HIV-1 strategies may well originate in part from inadequate assumptions built on bulk technologies, highlighting the need for new perspectives. The emergence of diverse single-cell technologies set the stage for potential breakthrough discoveries, as heterogeneous processes can now be investigated with an unprecedented depth in topics as diverse as HIV-1 tropism, dynamics of the replication cycle, latency, viral reservoirs and immune control. In this review, we summarize recent advances in the HIV-1 field made possible by single-cell technologies, and contextualize their importance. |
topic |
HIV-1 single-cell technologies pathogenesis cure vaccine single-cell omics |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2020.00297/full |
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