Staphylococcal Communities on Skin are Associated with Atopic Dermatitis and Disease Severity
The skin microbiota of atopic dermatitis (AD) patients is characterized by increased <i>Staphylococcus aureus</i> colonization, which exacerbates disease symptoms and has been linked to reduced bacterial diversity. Skin bacterial communities in AD patients have mostly been described at f...
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doaj-05b4b945e7b940f1b8ceaeb3cff017c42021-02-20T00:05:00ZengMDPI AGMicroorganisms2076-26072021-02-01943243210.3390/microorganisms9020432Staphylococcal Communities on Skin are Associated with Atopic Dermatitis and Disease SeveritySofie Marie Edslev0Caroline Meyer Olesen1Line Brok Nørreslet2Anna Cäcilia Ingham3Søren Iversen4Berit Lilje5Maja-Lisa Clausen6Jørgen Skov Jensen7Marc Stegger8Tove Agner9Paal Skytt Andersen10Bacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, DenmarkDepartment of Dermatology, Bispebjerg Hospital, Bispebjerg bakke 23, 2400 Copenhagen, DenmarkDepartment of Dermatology, Bispebjerg Hospital, Bispebjerg bakke 23, 2400 Copenhagen, DenmarkBacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, DenmarkBacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, DenmarkBacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, DenmarkDepartment of Dermatology, Bispebjerg Hospital, Bispebjerg bakke 23, 2400 Copenhagen, DenmarkBacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, DenmarkBacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, DenmarkDepartment of Dermatology, Bispebjerg Hospital, Bispebjerg bakke 23, 2400 Copenhagen, DenmarkBacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, DenmarkThe skin microbiota of atopic dermatitis (AD) patients is characterized by increased <i>Staphylococcus aureus</i> colonization, which exacerbates disease symptoms and has been linked to reduced bacterial diversity. Skin bacterial communities in AD patients have mostly been described at family and genus levels, while species-level characterization has been limited. In this study, we investigated the role of the bacteria belonging to the <i>Staphylococcus </i>genus using targeted sequencing of the <i>tuf</i> gene with genus-specific primers. We compared staphylococcal communities on lesional and non-lesional skin of AD patients, as well as AD patients with healthy controls, and determined the absolute abundance of bacteria present at each site. We observed that the staphylococcal community, bacterial alpha diversity, and bacterial densities were similar on lesional and non-lesional skin, whereas AD severity was associated with significant changes in staphylococcal composition. Increased <i>S. aureus</i>, <i>Staphylococcus capitis, </i>and <i>Staphylococcus lugdunensis </i>abundances were correlated with increased severity. Conversely, <i>Staphylococcus hominis </i>abundance was negatively correlated with severity. Furthermore, <i>S. hominis </i>relative abundance was reduced on AD skin compared to healthy skin. In conclusion, various staphylococcal species appear to be important for skin health.https://www.mdpi.com/2076-2607/9/2/432atopic dermatitisskin microbiomeskin microbiota<i>Staphylococcus</i><i>S. aureus</i>coagulase-negative staphylococci |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sofie Marie Edslev Caroline Meyer Olesen Line Brok Nørreslet Anna Cäcilia Ingham Søren Iversen Berit Lilje Maja-Lisa Clausen Jørgen Skov Jensen Marc Stegger Tove Agner Paal Skytt Andersen |
spellingShingle |
Sofie Marie Edslev Caroline Meyer Olesen Line Brok Nørreslet Anna Cäcilia Ingham Søren Iversen Berit Lilje Maja-Lisa Clausen Jørgen Skov Jensen Marc Stegger Tove Agner Paal Skytt Andersen Staphylococcal Communities on Skin are Associated with Atopic Dermatitis and Disease Severity Microorganisms atopic dermatitis skin microbiome skin microbiota <i>Staphylococcus</i> <i>S. aureus</i> coagulase-negative staphylococci |
author_facet |
Sofie Marie Edslev Caroline Meyer Olesen Line Brok Nørreslet Anna Cäcilia Ingham Søren Iversen Berit Lilje Maja-Lisa Clausen Jørgen Skov Jensen Marc Stegger Tove Agner Paal Skytt Andersen |
author_sort |
Sofie Marie Edslev |
title |
Staphylococcal Communities on Skin are Associated with Atopic Dermatitis and Disease Severity |
title_short |
Staphylococcal Communities on Skin are Associated with Atopic Dermatitis and Disease Severity |
title_full |
Staphylococcal Communities on Skin are Associated with Atopic Dermatitis and Disease Severity |
title_fullStr |
Staphylococcal Communities on Skin are Associated with Atopic Dermatitis and Disease Severity |
title_full_unstemmed |
Staphylococcal Communities on Skin are Associated with Atopic Dermatitis and Disease Severity |
title_sort |
staphylococcal communities on skin are associated with atopic dermatitis and disease severity |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-02-01 |
description |
The skin microbiota of atopic dermatitis (AD) patients is characterized by increased <i>Staphylococcus aureus</i> colonization, which exacerbates disease symptoms and has been linked to reduced bacterial diversity. Skin bacterial communities in AD patients have mostly been described at family and genus levels, while species-level characterization has been limited. In this study, we investigated the role of the bacteria belonging to the <i>Staphylococcus </i>genus using targeted sequencing of the <i>tuf</i> gene with genus-specific primers. We compared staphylococcal communities on lesional and non-lesional skin of AD patients, as well as AD patients with healthy controls, and determined the absolute abundance of bacteria present at each site. We observed that the staphylococcal community, bacterial alpha diversity, and bacterial densities were similar on lesional and non-lesional skin, whereas AD severity was associated with significant changes in staphylococcal composition. Increased <i>S. aureus</i>, <i>Staphylococcus capitis, </i>and <i>Staphylococcus lugdunensis </i>abundances were correlated with increased severity. Conversely, <i>Staphylococcus hominis </i>abundance was negatively correlated with severity. Furthermore, <i>S. hominis </i>relative abundance was reduced on AD skin compared to healthy skin. In conclusion, various staphylococcal species appear to be important for skin health. |
topic |
atopic dermatitis skin microbiome skin microbiota <i>Staphylococcus</i> <i>S. aureus</i> coagulase-negative staphylococci |
url |
https://www.mdpi.com/2076-2607/9/2/432 |
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