Role of morphine preconditioning and nitric oxide following brain ischemia reperfusion injury in mice
Objective(s): Morphine dependence (MD) potently protects heart against ischemia reperfusion (IR) injury through specific signaling mechanisms, which are different from the pathways involved in acute morphine treatment or classical preconditioning. Since opioid receptor density changes post cerebral...
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Mashhad University of Medical Sciences
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doaj-05cce1fac384410bb09fa92541edc7d42020-11-24T21:57:47ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences 2008-38662008-38742015-01-0118114213881Role of morphine preconditioning and nitric oxide following brain ischemia reperfusion injury in miceMaedeh Arabian0Nahid Aboutaleb1Mansoureh Soleimani2Fatemeh Zare Mehrjerdi3Marjan Ajami4Hamidreza Pazoki-Toroudi5Physiology Research Center, Physiology Department, Faculty of Medicine Iran University of Medical Sciences, Tehran, Iran Astronautics Research Institute, Iranian Space Research Center, Tehran, IranPhysiology Research Center, Physiology Department, Faculty of Medicine Iran University of Medical Sciences, Tehran, IranDepartment of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Physiology, Shahid Sadoughi University of Medical Sciences, Yazd, IranNutrition and Food Technology Research Institute, Faculty of Nutrition Science and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Physiology and Physiology Research Center, Iran University of Medical Sciences, Tehran, IranObjective(s): Morphine dependence (MD) potently protects heart against ischemia reperfusion (IR) injury through specific signaling mechanisms, which are different from the pathways involved in acute morphine treatment or classical preconditioning. Since opioid receptor density changes post cerebral ischemia strongly correlated with brain histological damage, in the present study, we tried to elucidate the possible role of opioid receptors in IR injury among morphine-dependent mice. Materials and Methods: Accordingly, incremental doses (10 mg/kg/day to 30 mg/kg/day) of morphine sulphate were subcutaneously administered for 5 days before global brain ischemia induction through bilateral common carotid artery occlusion. Animals were received naloxone (5 mg/kg) or L-NAME (20 mg/kg) 30 min after the last morphine dose. Twenty four hr after the ischemia induction, Retention trial of passive avoidance test and western blot analysis were done. histological analysis (TUNEL and NISSL staining) performed 72 hr after ischemia. Results: MD improved post ischemia memory performance (Phttp://ijbms.mums.ac.ir/pdf_3881_65a7dc8c14965742eda0ae1627ae72e0.htmlApoptosis Global brain ischemia Hippocampus Memory Morphine NO |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maedeh Arabian Nahid Aboutaleb Mansoureh Soleimani Fatemeh Zare Mehrjerdi Marjan Ajami Hamidreza Pazoki-Toroudi |
spellingShingle |
Maedeh Arabian Nahid Aboutaleb Mansoureh Soleimani Fatemeh Zare Mehrjerdi Marjan Ajami Hamidreza Pazoki-Toroudi Role of morphine preconditioning and nitric oxide following brain ischemia reperfusion injury in mice Iranian Journal of Basic Medical Sciences Apoptosis Global brain ischemia Hippocampus Memory Morphine NO |
author_facet |
Maedeh Arabian Nahid Aboutaleb Mansoureh Soleimani Fatemeh Zare Mehrjerdi Marjan Ajami Hamidreza Pazoki-Toroudi |
author_sort |
Maedeh Arabian |
title |
Role of morphine preconditioning and nitric oxide following brain ischemia reperfusion injury in mice |
title_short |
Role of morphine preconditioning and nitric oxide following brain ischemia reperfusion injury in mice |
title_full |
Role of morphine preconditioning and nitric oxide following brain ischemia reperfusion injury in mice |
title_fullStr |
Role of morphine preconditioning and nitric oxide following brain ischemia reperfusion injury in mice |
title_full_unstemmed |
Role of morphine preconditioning and nitric oxide following brain ischemia reperfusion injury in mice |
title_sort |
role of morphine preconditioning and nitric oxide following brain ischemia reperfusion injury in mice |
publisher |
Mashhad University of Medical Sciences |
series |
Iranian Journal of Basic Medical Sciences |
issn |
2008-3866 2008-3874 |
publishDate |
2015-01-01 |
description |
Objective(s): Morphine dependence (MD) potently protects heart against ischemia reperfusion (IR) injury through specific signaling mechanisms, which are different from the pathways involved in acute morphine treatment or classical preconditioning. Since opioid receptor density changes post cerebral ischemia strongly correlated with brain histological damage, in the present study, we tried to elucidate the possible role of opioid receptors in IR injury among morphine-dependent mice. Materials and Methods: Accordingly, incremental doses (10 mg/kg/day to 30 mg/kg/day) of morphine sulphate were subcutaneously administered for 5 days before global brain ischemia induction through bilateral common carotid artery occlusion. Animals were received naloxone (5 mg/kg) or L-NAME (20 mg/kg) 30 min after the last morphine dose. Twenty four hr after the ischemia induction, Retention trial of passive avoidance test and western blot analysis were done. histological analysis (TUNEL and NISSL staining) performed 72 hr after ischemia. Results: MD improved post ischemia memory performance (P |
topic |
Apoptosis Global brain ischemia Hippocampus Memory Morphine NO |
url |
http://ijbms.mums.ac.ir/pdf_3881_65a7dc8c14965742eda0ae1627ae72e0.html |
work_keys_str_mv |
AT maedeharabian roleofmorphinepreconditioningandnitricoxidefollowingbrainischemiareperfusioninjuryinmice AT nahidaboutaleb roleofmorphinepreconditioningandnitricoxidefollowingbrainischemiareperfusioninjuryinmice AT mansourehsoleimani roleofmorphinepreconditioningandnitricoxidefollowingbrainischemiareperfusioninjuryinmice AT fatemehzaremehrjerdi roleofmorphinepreconditioningandnitricoxidefollowingbrainischemiareperfusioninjuryinmice AT marjanajami roleofmorphinepreconditioningandnitricoxidefollowingbrainischemiareperfusioninjuryinmice AT hamidrezapazokitoroudi roleofmorphinepreconditioningandnitricoxidefollowingbrainischemiareperfusioninjuryinmice |
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