DEPTOR-mTOR Signaling Is Critical for Lipid Metabolism and Inflammation Homeostasis of Lymphocytes in Human PBMC Culture
Abnormal immune response of the body against substances and tissues causes autoimmune diseases, such as polymyositis, dermatomyositis, and rheumatoid arthritis. Irregular lipid metabolism and inflammation may be a significant cause of autoimmune diseases. Although much progress has been made, mechan...
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doaj-05e10ee38a7c416593c1e4d46e6619852020-11-25T01:43:17ZengHindawi LimitedJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/52528405252840DEPTOR-mTOR Signaling Is Critical for Lipid Metabolism and Inflammation Homeostasis of Lymphocytes in Human PBMC CultureQi-bing Xie0Yan Liang1Min Yang2Yuan Yang3Xiao-min Cen4Geng Yin5Department of Rheumatology and Immunology, West China Hospital of Sichuan University, Chengdu 610041, ChinaDepartment of Rheumatology and Immunology, West China Hospital of Sichuan University, Chengdu 610041, ChinaDepartment of Rheumatology and Immunology, West China Hospital of Sichuan University, Chengdu 610041, ChinaDepartment of Rheumatology and Immunology, West China Hospital of Sichuan University, Chengdu 610041, ChinaDepartment of Rheumatology and Immunology, West China Hospital of Sichuan University, Chengdu 610041, ChinaDepartment of General Practice, West China Hospital of Sichuan University, Chengdu 610041, ChinaAbnormal immune response of the body against substances and tissues causes autoimmune diseases, such as polymyositis, dermatomyositis, and rheumatoid arthritis. Irregular lipid metabolism and inflammation may be a significant cause of autoimmune diseases. Although much progress has been made, mechanisms of initiation and proceeding of metabolic and inflammatory regulation in autoimmune disease have not been well-defined. And novel markers for the detection and therapy of autoimmune disease are urgent. mTOR signaling is a central regulator of extracellular metabolic and inflammatory processes, while DEP domain-containing mTOR-interacting protein (DEPTOR) is a natural inhibitor of mTOR. Here, we report that overexpression of DEPTOR reduces mTORC1 activity in lymphocytes of human peripheral blood mononuclear cells (PBMCs). Combination of DEPTOR overexpression and mTORC2/AKT inhibitors effectively inhibits lipogenesis and inflammation in lymphocytes of PBMC culture. Moreover, DEPTOR knockdown activates mTORC1 and increases lipogenesis and inflammations. Our findings provide a deep insight into the relationship between lipid metabolism and inflammations via DEPTOR-mTOR pathway and imply that DEPTOR-mTOR in lymphocytes of PBMC culture has the potential to be as biomarkers for the detection and therapies of autoimmune diseases.http://dx.doi.org/10.1155/2017/5252840 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qi-bing Xie Yan Liang Min Yang Yuan Yang Xiao-min Cen Geng Yin |
spellingShingle |
Qi-bing Xie Yan Liang Min Yang Yuan Yang Xiao-min Cen Geng Yin DEPTOR-mTOR Signaling Is Critical for Lipid Metabolism and Inflammation Homeostasis of Lymphocytes in Human PBMC Culture Journal of Immunology Research |
author_facet |
Qi-bing Xie Yan Liang Min Yang Yuan Yang Xiao-min Cen Geng Yin |
author_sort |
Qi-bing Xie |
title |
DEPTOR-mTOR Signaling Is Critical for Lipid Metabolism and Inflammation Homeostasis of Lymphocytes in Human PBMC Culture |
title_short |
DEPTOR-mTOR Signaling Is Critical for Lipid Metabolism and Inflammation Homeostasis of Lymphocytes in Human PBMC Culture |
title_full |
DEPTOR-mTOR Signaling Is Critical for Lipid Metabolism and Inflammation Homeostasis of Lymphocytes in Human PBMC Culture |
title_fullStr |
DEPTOR-mTOR Signaling Is Critical for Lipid Metabolism and Inflammation Homeostasis of Lymphocytes in Human PBMC Culture |
title_full_unstemmed |
DEPTOR-mTOR Signaling Is Critical for Lipid Metabolism and Inflammation Homeostasis of Lymphocytes in Human PBMC Culture |
title_sort |
deptor-mtor signaling is critical for lipid metabolism and inflammation homeostasis of lymphocytes in human pbmc culture |
publisher |
Hindawi Limited |
series |
Journal of Immunology Research |
issn |
2314-8861 2314-7156 |
publishDate |
2017-01-01 |
description |
Abnormal immune response of the body against substances and tissues causes autoimmune diseases, such as polymyositis, dermatomyositis, and rheumatoid arthritis. Irregular lipid metabolism and inflammation may be a significant cause of autoimmune diseases. Although much progress has been made, mechanisms of initiation and proceeding of metabolic and inflammatory regulation in autoimmune disease have not been well-defined. And novel markers for the detection and therapy of autoimmune disease are urgent. mTOR signaling is a central regulator of extracellular metabolic and inflammatory processes, while DEP domain-containing mTOR-interacting protein (DEPTOR) is a natural inhibitor of mTOR. Here, we report that overexpression of DEPTOR reduces mTORC1 activity in lymphocytes of human peripheral blood mononuclear cells (PBMCs). Combination of DEPTOR overexpression and mTORC2/AKT inhibitors effectively inhibits lipogenesis and inflammation in lymphocytes of PBMC culture. Moreover, DEPTOR knockdown activates mTORC1 and increases lipogenesis and inflammations. Our findings provide a deep insight into the relationship between lipid metabolism and inflammations via DEPTOR-mTOR pathway and imply that DEPTOR-mTOR in lymphocytes of PBMC culture has the potential to be as biomarkers for the detection and therapies of autoimmune diseases. |
url |
http://dx.doi.org/10.1155/2017/5252840 |
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