Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease Individuals

This study investigated for the first time the genomewide DNA methylation changes of noncoding RNA genes in the temporal cortex samples from individuals with Alzheimer’s disease (AD). The methylome of 10 AD individuals and 10 age-matched controls were obtained using Illumina 450 K methylation array....

Full description

Bibliographic Details
Main Authors: Darine Villela, Rodrigo F. Ramalho, Aderbal R. T. Silva, Helena Brentani, Claudia K. Suemoto, Carlos Augusto Pasqualucci, Lea T. Grinberg, Ana C. V. Krepischi, Carla Rosenberg
Format: Article
Language:English
Published: Hindawi Limited 2016-01-01
Series:Neural Plasticity
Online Access:http://dx.doi.org/10.1155/2016/2584940
id doaj-0635c9bc44b945cda3593b867d3a1dfa
record_format Article
spelling doaj-0635c9bc44b945cda3593b867d3a1dfa2020-11-24T22:47:14ZengHindawi LimitedNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/25849402584940Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease IndividualsDarine Villela0Rodrigo F. Ramalho1Aderbal R. T. Silva2Helena Brentani3Claudia K. Suemoto4Carlos Augusto Pasqualucci5Lea T. Grinberg6Ana C. V. Krepischi7Carla Rosenberg8Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, Rua do Matão 277, 05508-090 São Paulo, SP, BrazilInternational Research Center, CIPE, AC Camargo Hospital, Rua Taguá 440, 01508-010 São Paulo, SP, BrazilInternational Research Center, CIPE, AC Camargo Hospital, Rua Taguá 440, 01508-010 São Paulo, SP, BrazilInstitute and Department of Psychiatry, University of São Paulo Medical School, Avenida Doutor Arnaldo 455, 01246-000 São Paulo, SP, BrazilDiscipline of Geriatrics, Department of Internal Medicine, University of São Paulo Medical School, Avenida Doutor Arnaldo 455, 01246-000 São Paulo, SP, BrazilBrazilian Aging Brain Study Group, LIM22, Department of Pathology, University of São Paulo Medical School, Avenida Doutor Arnaldo 455, 01246-000 São Paulo, SP, BrazilBrazilian Aging Brain Study Group, LIM22, Department of Pathology, University of São Paulo Medical School, Avenida Doutor Arnaldo 455, 01246-000 São Paulo, SP, BrazilDepartment of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, Rua do Matão 277, 05508-090 São Paulo, SP, BrazilDepartment of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, Rua do Matão 277, 05508-090 São Paulo, SP, BrazilThis study investigated for the first time the genomewide DNA methylation changes of noncoding RNA genes in the temporal cortex samples from individuals with Alzheimer’s disease (AD). The methylome of 10 AD individuals and 10 age-matched controls were obtained using Illumina 450 K methylation array. A total of 2,095 among the 15,258 interrogated noncoding RNA CpG sites presented differential methylation, 161 of which were associated with miRNA genes. In particular, 10 miRNA CpG sites that were found to be hypermethylated in AD compared to control brains represent transcripts that have been previously associated with the disease. This miRNA set is predicted to target 33 coding genes from the neuregulin receptor complex (ErbB) signaling pathway, which is required for the neurons myelination process. For 6 of these miRNA genes (MIR9-1, MIR9-3, MIR181C, MIR124-1, MIR146B, and MIR451), the hypermethylation pattern is in agreement with previous results from literature that shows downregulation of miR-9, miR-181c, miR-124, miR-146b, and miR-451 in the AD brain. Our data implicate dysregulation of miRNA methylation as contributor to the pathogenesis of AD.http://dx.doi.org/10.1155/2016/2584940
collection DOAJ
language English
format Article
sources DOAJ
author Darine Villela
Rodrigo F. Ramalho
Aderbal R. T. Silva
Helena Brentani
Claudia K. Suemoto
Carlos Augusto Pasqualucci
Lea T. Grinberg
Ana C. V. Krepischi
Carla Rosenberg
spellingShingle Darine Villela
Rodrigo F. Ramalho
Aderbal R. T. Silva
Helena Brentani
Claudia K. Suemoto
Carlos Augusto Pasqualucci
Lea T. Grinberg
Ana C. V. Krepischi
Carla Rosenberg
Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease Individuals
Neural Plasticity
author_facet Darine Villela
Rodrigo F. Ramalho
Aderbal R. T. Silva
Helena Brentani
Claudia K. Suemoto
Carlos Augusto Pasqualucci
Lea T. Grinberg
Ana C. V. Krepischi
Carla Rosenberg
author_sort Darine Villela
title Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease Individuals
title_short Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease Individuals
title_full Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease Individuals
title_fullStr Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease Individuals
title_full_unstemmed Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease Individuals
title_sort differential dna methylation of microrna genes in temporal cortex from alzheimer’s disease individuals
publisher Hindawi Limited
series Neural Plasticity
issn 2090-5904
1687-5443
publishDate 2016-01-01
description This study investigated for the first time the genomewide DNA methylation changes of noncoding RNA genes in the temporal cortex samples from individuals with Alzheimer’s disease (AD). The methylome of 10 AD individuals and 10 age-matched controls were obtained using Illumina 450 K methylation array. A total of 2,095 among the 15,258 interrogated noncoding RNA CpG sites presented differential methylation, 161 of which were associated with miRNA genes. In particular, 10 miRNA CpG sites that were found to be hypermethylated in AD compared to control brains represent transcripts that have been previously associated with the disease. This miRNA set is predicted to target 33 coding genes from the neuregulin receptor complex (ErbB) signaling pathway, which is required for the neurons myelination process. For 6 of these miRNA genes (MIR9-1, MIR9-3, MIR181C, MIR124-1, MIR146B, and MIR451), the hypermethylation pattern is in agreement with previous results from literature that shows downregulation of miR-9, miR-181c, miR-124, miR-146b, and miR-451 in the AD brain. Our data implicate dysregulation of miRNA methylation as contributor to the pathogenesis of AD.
url http://dx.doi.org/10.1155/2016/2584940
work_keys_str_mv AT darinevillela differentialdnamethylationofmicrornagenesintemporalcortexfromalzheimersdiseaseindividuals
AT rodrigoframalho differentialdnamethylationofmicrornagenesintemporalcortexfromalzheimersdiseaseindividuals
AT aderbalrtsilva differentialdnamethylationofmicrornagenesintemporalcortexfromalzheimersdiseaseindividuals
AT helenabrentani differentialdnamethylationofmicrornagenesintemporalcortexfromalzheimersdiseaseindividuals
AT claudiaksuemoto differentialdnamethylationofmicrornagenesintemporalcortexfromalzheimersdiseaseindividuals
AT carlosaugustopasqualucci differentialdnamethylationofmicrornagenesintemporalcortexfromalzheimersdiseaseindividuals
AT leatgrinberg differentialdnamethylationofmicrornagenesintemporalcortexfromalzheimersdiseaseindividuals
AT anacvkrepischi differentialdnamethylationofmicrornagenesintemporalcortexfromalzheimersdiseaseindividuals
AT carlarosenberg differentialdnamethylationofmicrornagenesintemporalcortexfromalzheimersdiseaseindividuals
_version_ 1725682347262607360