Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease Individuals
This study investigated for the first time the genomewide DNA methylation changes of noncoding RNA genes in the temporal cortex samples from individuals with Alzheimer’s disease (AD). The methylome of 10 AD individuals and 10 age-matched controls were obtained using Illumina 450 K methylation array....
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doaj-0635c9bc44b945cda3593b867d3a1dfa2020-11-24T22:47:14ZengHindawi LimitedNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/25849402584940Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease IndividualsDarine Villela0Rodrigo F. Ramalho1Aderbal R. T. Silva2Helena Brentani3Claudia K. Suemoto4Carlos Augusto Pasqualucci5Lea T. Grinberg6Ana C. V. Krepischi7Carla Rosenberg8Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, Rua do Matão 277, 05508-090 São Paulo, SP, BrazilInternational Research Center, CIPE, AC Camargo Hospital, Rua Taguá 440, 01508-010 São Paulo, SP, BrazilInternational Research Center, CIPE, AC Camargo Hospital, Rua Taguá 440, 01508-010 São Paulo, SP, BrazilInstitute and Department of Psychiatry, University of São Paulo Medical School, Avenida Doutor Arnaldo 455, 01246-000 São Paulo, SP, BrazilDiscipline of Geriatrics, Department of Internal Medicine, University of São Paulo Medical School, Avenida Doutor Arnaldo 455, 01246-000 São Paulo, SP, BrazilBrazilian Aging Brain Study Group, LIM22, Department of Pathology, University of São Paulo Medical School, Avenida Doutor Arnaldo 455, 01246-000 São Paulo, SP, BrazilBrazilian Aging Brain Study Group, LIM22, Department of Pathology, University of São Paulo Medical School, Avenida Doutor Arnaldo 455, 01246-000 São Paulo, SP, BrazilDepartment of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, Rua do Matão 277, 05508-090 São Paulo, SP, BrazilDepartment of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, Rua do Matão 277, 05508-090 São Paulo, SP, BrazilThis study investigated for the first time the genomewide DNA methylation changes of noncoding RNA genes in the temporal cortex samples from individuals with Alzheimer’s disease (AD). The methylome of 10 AD individuals and 10 age-matched controls were obtained using Illumina 450 K methylation array. A total of 2,095 among the 15,258 interrogated noncoding RNA CpG sites presented differential methylation, 161 of which were associated with miRNA genes. In particular, 10 miRNA CpG sites that were found to be hypermethylated in AD compared to control brains represent transcripts that have been previously associated with the disease. This miRNA set is predicted to target 33 coding genes from the neuregulin receptor complex (ErbB) signaling pathway, which is required for the neurons myelination process. For 6 of these miRNA genes (MIR9-1, MIR9-3, MIR181C, MIR124-1, MIR146B, and MIR451), the hypermethylation pattern is in agreement with previous results from literature that shows downregulation of miR-9, miR-181c, miR-124, miR-146b, and miR-451 in the AD brain. Our data implicate dysregulation of miRNA methylation as contributor to the pathogenesis of AD.http://dx.doi.org/10.1155/2016/2584940 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Darine Villela Rodrigo F. Ramalho Aderbal R. T. Silva Helena Brentani Claudia K. Suemoto Carlos Augusto Pasqualucci Lea T. Grinberg Ana C. V. Krepischi Carla Rosenberg |
spellingShingle |
Darine Villela Rodrigo F. Ramalho Aderbal R. T. Silva Helena Brentani Claudia K. Suemoto Carlos Augusto Pasqualucci Lea T. Grinberg Ana C. V. Krepischi Carla Rosenberg Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease Individuals Neural Plasticity |
author_facet |
Darine Villela Rodrigo F. Ramalho Aderbal R. T. Silva Helena Brentani Claudia K. Suemoto Carlos Augusto Pasqualucci Lea T. Grinberg Ana C. V. Krepischi Carla Rosenberg |
author_sort |
Darine Villela |
title |
Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease Individuals |
title_short |
Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease Individuals |
title_full |
Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease Individuals |
title_fullStr |
Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease Individuals |
title_full_unstemmed |
Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer’s Disease Individuals |
title_sort |
differential dna methylation of microrna genes in temporal cortex from alzheimer’s disease individuals |
publisher |
Hindawi Limited |
series |
Neural Plasticity |
issn |
2090-5904 1687-5443 |
publishDate |
2016-01-01 |
description |
This study investigated for the first time the genomewide DNA methylation changes of noncoding RNA genes in the temporal cortex samples from individuals with Alzheimer’s disease (AD). The methylome of 10 AD individuals and 10 age-matched controls were obtained using Illumina 450 K methylation array. A total of 2,095 among the 15,258 interrogated noncoding RNA CpG sites presented differential methylation, 161 of which were associated with miRNA genes. In particular, 10 miRNA CpG sites that were found to be hypermethylated in AD compared to control brains represent transcripts that have been previously associated with the disease. This miRNA set is predicted to target 33 coding genes from the neuregulin receptor complex (ErbB) signaling pathway, which is required for the neurons myelination process. For 6 of these miRNA genes (MIR9-1, MIR9-3, MIR181C, MIR124-1, MIR146B, and MIR451), the hypermethylation pattern is in agreement with previous results from literature that shows downregulation of miR-9, miR-181c, miR-124, miR-146b, and miR-451 in the AD brain. Our data implicate dysregulation of miRNA methylation as contributor to the pathogenesis of AD. |
url |
http://dx.doi.org/10.1155/2016/2584940 |
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