Simultaneous identification of abused drugs, benzodiazepines, and new psychoactive substances in urine by liquid chromatography tandem mass spectrometry
A literature search reveals no studies concerning simultaneous identification of commonly abused drugs, benzodiazepines, and new psychoactive substances in urine by liquid chromatography tandem mass spectrometry (LC–MS/MS). We developed and validated an LC–MS/MS method for simultaneous identificatio...
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doaj-063b6ab959b74f2b95568734f4b24bba2020-11-25T02:43:27ZengWileyKaohsiung Journal of Medical Sciences1607-551X2016-03-0132311812710.1016/j.kjms.2016.01.007Simultaneous identification of abused drugs, benzodiazepines, and new psychoactive substances in urine by liquid chromatography tandem mass spectrometryHei-Hwa Lee0Jong-Feng Lee1Shin-Yu Lin2Bai-Hsiun Chen3Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung, TaiwanDepartment of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung, TaiwanDepartment of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung, TaiwanDepartment of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung, TaiwanA literature search reveals no studies concerning simultaneous identification of commonly abused drugs, benzodiazepines, and new psychoactive substances in urine by liquid chromatography tandem mass spectrometry (LC–MS/MS). We developed and validated an LC–MS/MS method for simultaneous identification of multiple abused drugs, benzodiazepines, and new psychoactive substances in urine from suspected drug abusers. The instrument was operated in multiple-reaction monitoring using an electrospray ionization mode. Chromatograms were separated using an ACE5 C18 column on a gradient of acetonitrile. After liquid–liquid extraction, samples were passed through a 0.22-μm polyvinylidene difluoride filter before injection into the LC–MS/MS. The limits of quantitation ranged from 0.5 ng/mL to 31.3 ng/mL. The linearity ranged from 0.5 ng/mL to 200 ng/mL. The precision results were below 15.4% (intraday) and 18.7% (interday). The intraday accuracy ranged from 85.9% to 121.0%; interday accuracy ranged from 66.1% to 128.7%. The proposed method was applied to 769 urine samples. The most common three drugs identified were ketamine, amphetamine, and opiates. The drug positive rate for one or more drugs was 79.6%. Our results demonstrate the suitability of the LC–MS/MS method for simultaneous identification of multiple abused drugs, benzodiazepines, and new psychoactive substances in urine.http://www.sciencedirect.com/science/article/pii/S1607551X16000085BenzodiazepinesDrug abuseLC–MS/MSNew psychoactive substances |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hei-Hwa Lee Jong-Feng Lee Shin-Yu Lin Bai-Hsiun Chen |
spellingShingle |
Hei-Hwa Lee Jong-Feng Lee Shin-Yu Lin Bai-Hsiun Chen Simultaneous identification of abused drugs, benzodiazepines, and new psychoactive substances in urine by liquid chromatography tandem mass spectrometry Kaohsiung Journal of Medical Sciences Benzodiazepines Drug abuse LC–MS/MS New psychoactive substances |
author_facet |
Hei-Hwa Lee Jong-Feng Lee Shin-Yu Lin Bai-Hsiun Chen |
author_sort |
Hei-Hwa Lee |
title |
Simultaneous identification of abused drugs, benzodiazepines, and new psychoactive substances in urine by liquid chromatography tandem mass spectrometry |
title_short |
Simultaneous identification of abused drugs, benzodiazepines, and new psychoactive substances in urine by liquid chromatography tandem mass spectrometry |
title_full |
Simultaneous identification of abused drugs, benzodiazepines, and new psychoactive substances in urine by liquid chromatography tandem mass spectrometry |
title_fullStr |
Simultaneous identification of abused drugs, benzodiazepines, and new psychoactive substances in urine by liquid chromatography tandem mass spectrometry |
title_full_unstemmed |
Simultaneous identification of abused drugs, benzodiazepines, and new psychoactive substances in urine by liquid chromatography tandem mass spectrometry |
title_sort |
simultaneous identification of abused drugs, benzodiazepines, and new psychoactive substances in urine by liquid chromatography tandem mass spectrometry |
publisher |
Wiley |
series |
Kaohsiung Journal of Medical Sciences |
issn |
1607-551X |
publishDate |
2016-03-01 |
description |
A literature search reveals no studies concerning simultaneous identification of commonly abused drugs, benzodiazepines, and new psychoactive substances in urine by liquid chromatography tandem mass spectrometry (LC–MS/MS). We developed and validated an LC–MS/MS method for simultaneous identification of multiple abused drugs, benzodiazepines, and new psychoactive substances in urine from suspected drug abusers. The instrument was operated in multiple-reaction monitoring using an electrospray ionization mode. Chromatograms were separated using an ACE5 C18 column on a gradient of acetonitrile. After liquid–liquid extraction, samples were passed through a 0.22-μm polyvinylidene difluoride filter before injection into the LC–MS/MS. The limits of quantitation ranged from 0.5 ng/mL to 31.3 ng/mL. The linearity ranged from 0.5 ng/mL to 200 ng/mL. The precision results were below 15.4% (intraday) and 18.7% (interday). The intraday accuracy ranged from 85.9% to 121.0%; interday accuracy ranged from 66.1% to 128.7%. The proposed method was applied to 769 urine samples. The most common three drugs identified were ketamine, amphetamine, and opiates. The drug positive rate for one or more drugs was 79.6%. Our results demonstrate the suitability of the LC–MS/MS method for simultaneous identification of multiple abused drugs, benzodiazepines, and new psychoactive substances in urine. |
topic |
Benzodiazepines Drug abuse LC–MS/MS New psychoactive substances |
url |
http://www.sciencedirect.com/science/article/pii/S1607551X16000085 |
work_keys_str_mv |
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