Kaposi’s Sarcoma-Associated Herpesvirus Reactivation by Targeting of a dCas9-Based Transcription Activator to the ORF50 Promoter

Kaposi’s Sarcoma-Associated Herpesvirus Reactivation by Targeting of a dCas9-Based Transcription Activator to the ORF50 Promoter

CRISPR activation (CRISPRa) has revealed great potential as a tool to modulate the expression of targeted cellular genes. Here, we successfully applied the CRISPRa system to trigger the Kaposi’s sarcoma-associated herpesvirus (KSHV) reactivation in latently infected cells by selectively activating O...

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Bibliographic Details
Main Authors: Endrit Elbasani, Francesca Falasco, Silvia Gramolelli, Veijo Nurminen, Thomas Günther, Jere Weltner, Diego Balboa, Adam Grundhoff, Timo Otonkoski, Päivi M. Ojala
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Viruses
Subjects:
KSHV
dCas9
CRISPRa
DD-dCas9-VP192
ORF50
RTA
Online Access:https://www.mdpi.com/1999-4915/12/9/952

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