Avoiding common numts to provide reliable species identification for tiger parts

Tigers are killed to supply a demand for many wildlife products despite a ban on commercial international trade. As populations decrease, products from substitute species (i.e. lions and leopards) have been fraudulently sold as tiger. DNA forensic techniques are needed to definitively identify tiger...

Full description

Bibliographic Details
Main Authors:	Kelly I. Morgan, Kyle M. Ewart, Truong Q. Nguyen, Frankie T. Sitam, Kanita Ouitavon, Amanda L. Lightson, Antoinette Kotze, Ross McEwing
Format:	Article
Language:	English
Published:	Elsevier 2021-07-01
Series:	Forensic Science International: Reports
Subjects:	Cytochrome oxidase subunit 1 Numt Pseudogene Species identification Tiger trade Wildlife forensic science
Online Access:	http://www.sciencedirect.com/science/article/pii/S266591072030116X

id	doaj-064178f7fdf64a12a4023d8daf291804
record_format	Article
spelling	doaj-064178f7fdf64a12a4023d8daf2918042021-08-12T04:35:30ZengElsevierForensic Science International: Reports2665-91072021-07-013100166Avoiding common numts to provide reliable species identification for tiger partsKelly I. Morgan0Kyle M. Ewart1Truong Q. Nguyen2Frankie T. Sitam3Kanita Ouitavon4Amanda L. Lightson5Antoinette Kotze6Ross McEwing7TRACE Wildlife Forensics Network, Edinburgh, Scotland, United Kingdom; Corresponding author.TRACE Wildlife Forensics Network, Edinburgh, Scotland, United KingdomInstitute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Viet NamNational Wildlife Forensic Laboratory (NWFL), Department of Wildlife and National Parks (PERHILITAN), Kuala Lumpur, MalaysiaDNP-WIFOS Laboratory Center, Department of National Parks, Wildlife and Plant Conservation, Bangkok, ThailandTRACE Wildlife Forensics Network, Edinburgh, Scotland, United KingdomSouth African National Biodiversity Institute (SANBI), Pretoria, South Africa; University of the Free State, Genetics Department, Bloemfontein, South AfricaTRACE Wildlife Forensics Network, Edinburgh, Scotland, United KingdomTigers are killed to supply a demand for many wildlife products despite a ban on commercial international trade. As populations decrease, products from substitute species (i.e. lions and leopards) have been fraudulently sold as tiger. DNA forensic techniques are needed to definitively identify tiger in order to secure prosecutions although this is complicated by the presence of numts. Therefore, we have developed and validated a CO1 genetic marker that preferentially amplifies the mtDNA CO1 region and excludes the nuclear CO1 pseudogene, which we expect to be of use in tiger forensic casework.http://www.sciencedirect.com/science/article/pii/S266591072030116XCytochrome oxidase subunit 1NumtPseudogeneSpecies identificationTiger tradeWildlife forensic science
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Kelly I. Morgan Kyle M. Ewart Truong Q. Nguyen Frankie T. Sitam Kanita Ouitavon Amanda L. Lightson Antoinette Kotze Ross McEwing
spellingShingle	Kelly I. Morgan Kyle M. Ewart Truong Q. Nguyen Frankie T. Sitam Kanita Ouitavon Amanda L. Lightson Antoinette Kotze Ross McEwing Avoiding common numts to provide reliable species identification for tiger parts Forensic Science International: Reports Cytochrome oxidase subunit 1 Numt Pseudogene Species identification Tiger trade Wildlife forensic science
author_facet	Kelly I. Morgan Kyle M. Ewart Truong Q. Nguyen Frankie T. Sitam Kanita Ouitavon Amanda L. Lightson Antoinette Kotze Ross McEwing
author_sort	Kelly I. Morgan
title	Avoiding common numts to provide reliable species identification for tiger parts
title_short	Avoiding common numts to provide reliable species identification for tiger parts
title_full	Avoiding common numts to provide reliable species identification for tiger parts
title_fullStr	Avoiding common numts to provide reliable species identification for tiger parts
title_full_unstemmed	Avoiding common numts to provide reliable species identification for tiger parts
title_sort	avoiding common numts to provide reliable species identification for tiger parts
publisher	Elsevier
series	Forensic Science International: Reports
issn	2665-9107
publishDate	2021-07-01
description	Tigers are killed to supply a demand for many wildlife products despite a ban on commercial international trade. As populations decrease, products from substitute species (i.e. lions and leopards) have been fraudulently sold as tiger. DNA forensic techniques are needed to definitively identify tiger in order to secure prosecutions although this is complicated by the presence of numts. Therefore, we have developed and validated a CO1 genetic marker that preferentially amplifies the mtDNA CO1 region and excludes the nuclear CO1 pseudogene, which we expect to be of use in tiger forensic casework.
topic	Cytochrome oxidase subunit 1 Numt Pseudogene Species identification Tiger trade Wildlife forensic science
url	http://www.sciencedirect.com/science/article/pii/S266591072030116X
work_keys_str_mv	AT kellyimorgan avoidingcommonnumtstoprovidereliablespeciesidentificationfortigerparts AT kylemewart avoidingcommonnumtstoprovidereliablespeciesidentificationfortigerparts AT truongqnguyen avoidingcommonnumtstoprovidereliablespeciesidentificationfortigerparts AT frankietsitam avoidingcommonnumtstoprovidereliablespeciesidentificationfortigerparts AT kanitaouitavon avoidingcommonnumtstoprovidereliablespeciesidentificationfortigerparts AT amandallightson avoidingcommonnumtstoprovidereliablespeciesidentificationfortigerparts AT antoinettekotze avoidingcommonnumtstoprovidereliablespeciesidentificationfortigerparts AT rossmcewing avoidingcommonnumtstoprovidereliablespeciesidentificationfortigerparts
_version_	1721209730975662080

Avoiding common numts to provide reliable species identification for tiger parts

Similar Items