Avoiding common numts to provide reliable species identification for tiger parts
Tigers are killed to supply a demand for many wildlife products despite a ban on commercial international trade. As populations decrease, products from substitute species (i.e. lions and leopards) have been fraudulently sold as tiger. DNA forensic techniques are needed to definitively identify tiger...
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doaj-064178f7fdf64a12a4023d8daf2918042021-08-12T04:35:30ZengElsevierForensic Science International: Reports2665-91072021-07-013100166Avoiding common numts to provide reliable species identification for tiger partsKelly I. Morgan0Kyle M. Ewart1Truong Q. Nguyen2Frankie T. Sitam3Kanita Ouitavon4Amanda L. Lightson5Antoinette Kotze6Ross McEwing7TRACE Wildlife Forensics Network, Edinburgh, Scotland, United Kingdom; Corresponding author.TRACE Wildlife Forensics Network, Edinburgh, Scotland, United KingdomInstitute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Viet NamNational Wildlife Forensic Laboratory (NWFL), Department of Wildlife and National Parks (PERHILITAN), Kuala Lumpur, MalaysiaDNP-WIFOS Laboratory Center, Department of National Parks, Wildlife and Plant Conservation, Bangkok, ThailandTRACE Wildlife Forensics Network, Edinburgh, Scotland, United KingdomSouth African National Biodiversity Institute (SANBI), Pretoria, South Africa; University of the Free State, Genetics Department, Bloemfontein, South AfricaTRACE Wildlife Forensics Network, Edinburgh, Scotland, United KingdomTigers are killed to supply a demand for many wildlife products despite a ban on commercial international trade. As populations decrease, products from substitute species (i.e. lions and leopards) have been fraudulently sold as tiger. DNA forensic techniques are needed to definitively identify tiger in order to secure prosecutions although this is complicated by the presence of numts. Therefore, we have developed and validated a CO1 genetic marker that preferentially amplifies the mtDNA CO1 region and excludes the nuclear CO1 pseudogene, which we expect to be of use in tiger forensic casework.http://www.sciencedirect.com/science/article/pii/S266591072030116XCytochrome oxidase subunit 1NumtPseudogeneSpecies identificationTiger tradeWildlife forensic science |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kelly I. Morgan Kyle M. Ewart Truong Q. Nguyen Frankie T. Sitam Kanita Ouitavon Amanda L. Lightson Antoinette Kotze Ross McEwing |
spellingShingle |
Kelly I. Morgan Kyle M. Ewart Truong Q. Nguyen Frankie T. Sitam Kanita Ouitavon Amanda L. Lightson Antoinette Kotze Ross McEwing Avoiding common numts to provide reliable species identification for tiger parts Forensic Science International: Reports Cytochrome oxidase subunit 1 Numt Pseudogene Species identification Tiger trade Wildlife forensic science |
author_facet |
Kelly I. Morgan Kyle M. Ewart Truong Q. Nguyen Frankie T. Sitam Kanita Ouitavon Amanda L. Lightson Antoinette Kotze Ross McEwing |
author_sort |
Kelly I. Morgan |
title |
Avoiding common numts to provide reliable species identification for tiger parts |
title_short |
Avoiding common numts to provide reliable species identification for tiger parts |
title_full |
Avoiding common numts to provide reliable species identification for tiger parts |
title_fullStr |
Avoiding common numts to provide reliable species identification for tiger parts |
title_full_unstemmed |
Avoiding common numts to provide reliable species identification for tiger parts |
title_sort |
avoiding common numts to provide reliable species identification for tiger parts |
publisher |
Elsevier |
series |
Forensic Science International: Reports |
issn |
2665-9107 |
publishDate |
2021-07-01 |
description |
Tigers are killed to supply a demand for many wildlife products despite a ban on commercial international trade. As populations decrease, products from substitute species (i.e. lions and leopards) have been fraudulently sold as tiger. DNA forensic techniques are needed to definitively identify tiger in order to secure prosecutions although this is complicated by the presence of numts. Therefore, we have developed and validated a CO1 genetic marker that preferentially amplifies the mtDNA CO1 region and excludes the nuclear CO1 pseudogene, which we expect to be of use in tiger forensic casework. |
topic |
Cytochrome oxidase subunit 1 Numt Pseudogene Species identification Tiger trade Wildlife forensic science |
url |
http://www.sciencedirect.com/science/article/pii/S266591072030116X |
work_keys_str_mv |
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