Prototheca zopfii genotype 2 disseminated infection in a dog with neurological signs
ABSTRACT: Prototheca zopfii is an alga that can cause disease in animals and humans. Here, we reported a case of systemic protothecosis in a 2-year-old female Boxer. The animal exhibited tetraparesis and vertical nystagmus. It died two weeks after the onset of clinical signs and was necropsied. At g...
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Universidade Federal de Santa Maria
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doaj-0641bdafa16a46f6bef87396717161082020-11-24T22:34:35ZengUniversidade Federal de Santa MariaCiência Rural1678-459647810.1590/0103-8478cr20160877S0103-84782017000800551Prototheca zopfii genotype 2 disseminated infection in a dog with neurological signsLuciana SonneEduardo Conceição de OliveiraFernando Froner ArgentaRafael Souza MonteggiaLaerte FerreiroUwe RöslerOlivia KershawDavid DriemeierABSTRACT: Prototheca zopfii is an alga that can cause disease in animals and humans. Here, we reported a case of systemic protothecosis in a 2-year-old female Boxer. The animal exhibited tetraparesis and vertical nystagmus. It died two weeks after the onset of clinical signs and was necropsied. At gross examination, whitish areas were identified in the heart. Oval or round structures were observed by microscopic examination, some of which formed morula-like structures compatible with algae in the heart, skeletal muscle, and brain. Growth of Prototheca sp. was observed in the heart after culture at 37°C on Sabouraud agar medium with chloramphenicol. Immunohistochemical analysis of the heart and brain using an anti-Prototheca zopfii polyclonal antibody yielded positive results. Genotyping of the cultured agent from brain and heart samples was performed by restriction fragment length polymorphism of a specific 18S rDNA fragment. P. zopfii genotype 2 was reported to be the cause of disseminated protothecosis in this dog with manifestation in the brain, heart, and skeletal muscle.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000800551&lng=en&tlng=encaninoprototecosePrototheca zopfii genótipo 2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Luciana Sonne Eduardo Conceição de Oliveira Fernando Froner Argenta Rafael Souza Monteggia Laerte Ferreiro Uwe Rösler Olivia Kershaw David Driemeier |
spellingShingle |
Luciana Sonne Eduardo Conceição de Oliveira Fernando Froner Argenta Rafael Souza Monteggia Laerte Ferreiro Uwe Rösler Olivia Kershaw David Driemeier Prototheca zopfii genotype 2 disseminated infection in a dog with neurological signs Ciência Rural canino prototecose Prototheca zopfii genótipo 2 |
author_facet |
Luciana Sonne Eduardo Conceição de Oliveira Fernando Froner Argenta Rafael Souza Monteggia Laerte Ferreiro Uwe Rösler Olivia Kershaw David Driemeier |
author_sort |
Luciana Sonne |
title |
Prototheca zopfii genotype 2 disseminated infection in a dog with neurological signs |
title_short |
Prototheca zopfii genotype 2 disseminated infection in a dog with neurological signs |
title_full |
Prototheca zopfii genotype 2 disseminated infection in a dog with neurological signs |
title_fullStr |
Prototheca zopfii genotype 2 disseminated infection in a dog with neurological signs |
title_full_unstemmed |
Prototheca zopfii genotype 2 disseminated infection in a dog with neurological signs |
title_sort |
prototheca zopfii genotype 2 disseminated infection in a dog with neurological signs |
publisher |
Universidade Federal de Santa Maria |
series |
Ciência Rural |
issn |
1678-4596 |
description |
ABSTRACT: Prototheca zopfii is an alga that can cause disease in animals and humans. Here, we reported a case of systemic protothecosis in a 2-year-old female Boxer. The animal exhibited tetraparesis and vertical nystagmus. It died two weeks after the onset of clinical signs and was necropsied. At gross examination, whitish areas were identified in the heart. Oval or round structures were observed by microscopic examination, some of which formed morula-like structures compatible with algae in the heart, skeletal muscle, and brain. Growth of Prototheca sp. was observed in the heart after culture at 37°C on Sabouraud agar medium with chloramphenicol. Immunohistochemical analysis of the heart and brain using an anti-Prototheca zopfii polyclonal antibody yielded positive results. Genotyping of the cultured agent from brain and heart samples was performed by restriction fragment length polymorphism of a specific 18S rDNA fragment. P. zopfii genotype 2 was reported to be the cause of disseminated protothecosis in this dog with manifestation in the brain, heart, and skeletal muscle. |
topic |
canino prototecose Prototheca zopfii genótipo 2 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000800551&lng=en&tlng=en |
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