Evaluation of the dioxin-like toxicity in soil samples from Thua Thien Hue province using the AhR-CALUX bioassay – An update of Agent Orange contamination in Vietnam
In this study, an AhR-responsive reporter-gene cell-based bioassay CALUX was used to assess the biological potency of dioxins and dioxin-like PCBs (dl-PCBs) in top soil samples collected from a former airbase (A-So) and remote regions from urban and agricultural areas in Thua Thien Hue, Vietnam. In...
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doaj-0642f94c5ca84692acd8a12e330d26302021-04-23T06:16:14ZengElsevierEcotoxicology and Environmental Safety0147-65132021-04-01212111971Evaluation of the dioxin-like toxicity in soil samples from Thua Thien Hue province using the AhR-CALUX bioassay – An update of Agent Orange contamination in VietnamTran Thi Ai My0Nguyen Duy Dat1Kersten Van Langenhove2Michael S. Denison3Hoang Thai Long4Marc Elskens5Analytical, Environmental and Geo-Chemistry laboratory (AMGC), Vrije Universiteit Brussel (University of Brussels), Pleinlaan 2, 1050 Brussels, Belgium; Department of Chemistry, University of Sciences, Hue University, Hue 530000, Vietnam; Corresponding author at: Department of Chemistry, University of Sciences, Hue University, Hue 530000, Vietnam.Faculty of Chemical & Food Technology, University of Technology and Education, Thu Duc, Ho Chi Minh 700000, VietnamAnalytical, Environmental and Geo-Chemistry laboratory (AMGC), Vrije Universiteit Brussel (University of Brussels), Pleinlaan 2, 1050 Brussels, BelgiumUniversity of California, Davis (UC Davis), Department of Environmental Toxicology, One Shields Avenue, Davis, CA 95616, USADepartment of Chemistry, University of Sciences, Hue University, Hue 530000, VietnamAnalytical, Environmental and Geo-Chemistry laboratory (AMGC), Vrije Universiteit Brussel (University of Brussels), Pleinlaan 2, 1050 Brussels, BelgiumIn this study, an AhR-responsive reporter-gene cell-based bioassay CALUX was used to assess the biological potency of dioxins and dioxin-like PCBs (dl-PCBs) in top soil samples collected from a former airbase (A-So) and remote regions from urban and agricultural areas in Thua Thien Hue, Vietnam. In top soil collected from A-So airbase, Bioanalytical EQuivalent (BEQ) concentrations of up to 2700 pg 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) per g dry weight (pg BEQ-TCDD g−1 dw) were assessed. Interestingly, while BEQ values for dl-PCBs were found to be up to 13 pg BEQ-TCDD g−1 dw, the dl-PCB activity was not detected in all the hotspot sample extracts. In contrasts, BEQ values for dioxin like compounds from remote regions were much lower and occasionally below the quantification limits of the method. The BEQ activities obtained in this study have a similar trend to the WHO-TEQ results for the samples collected in the A-So airbase. However, BEQ values were higher than those of TEQ, probably reflecting the presence of additional AhR ligands and/or possible non-additive interactions in the sample mixture. This study confirms that after more than 60 years, a strong residual pollution of PCDD/Fs remains on this former air base following the use and storage of Agent Orange during the Vietnam War, raising a health risk for populations exposed in this area because livestock animals graze there.http://www.sciencedirect.com/science/article/pii/S0147651321000828CALUX bioassayPCDD/FsDioxin-like PCBsA-So former airbase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tran Thi Ai My Nguyen Duy Dat Kersten Van Langenhove Michael S. Denison Hoang Thai Long Marc Elskens |
spellingShingle |
Tran Thi Ai My Nguyen Duy Dat Kersten Van Langenhove Michael S. Denison Hoang Thai Long Marc Elskens Evaluation of the dioxin-like toxicity in soil samples from Thua Thien Hue province using the AhR-CALUX bioassay – An update of Agent Orange contamination in Vietnam Ecotoxicology and Environmental Safety CALUX bioassay PCDD/Fs Dioxin-like PCBs A-So former airbase |
author_facet |
Tran Thi Ai My Nguyen Duy Dat Kersten Van Langenhove Michael S. Denison Hoang Thai Long Marc Elskens |
author_sort |
Tran Thi Ai My |
title |
Evaluation of the dioxin-like toxicity in soil samples from Thua Thien Hue province using the AhR-CALUX bioassay – An update of Agent Orange contamination in Vietnam |
title_short |
Evaluation of the dioxin-like toxicity in soil samples from Thua Thien Hue province using the AhR-CALUX bioassay – An update of Agent Orange contamination in Vietnam |
title_full |
Evaluation of the dioxin-like toxicity in soil samples from Thua Thien Hue province using the AhR-CALUX bioassay – An update of Agent Orange contamination in Vietnam |
title_fullStr |
Evaluation of the dioxin-like toxicity in soil samples from Thua Thien Hue province using the AhR-CALUX bioassay – An update of Agent Orange contamination in Vietnam |
title_full_unstemmed |
Evaluation of the dioxin-like toxicity in soil samples from Thua Thien Hue province using the AhR-CALUX bioassay – An update of Agent Orange contamination in Vietnam |
title_sort |
evaluation of the dioxin-like toxicity in soil samples from thua thien hue province using the ahr-calux bioassay – an update of agent orange contamination in vietnam |
publisher |
Elsevier |
series |
Ecotoxicology and Environmental Safety |
issn |
0147-6513 |
publishDate |
2021-04-01 |
description |
In this study, an AhR-responsive reporter-gene cell-based bioassay CALUX was used to assess the biological potency of dioxins and dioxin-like PCBs (dl-PCBs) in top soil samples collected from a former airbase (A-So) and remote regions from urban and agricultural areas in Thua Thien Hue, Vietnam. In top soil collected from A-So airbase, Bioanalytical EQuivalent (BEQ) concentrations of up to 2700 pg 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) per g dry weight (pg BEQ-TCDD g−1 dw) were assessed. Interestingly, while BEQ values for dl-PCBs were found to be up to 13 pg BEQ-TCDD g−1 dw, the dl-PCB activity was not detected in all the hotspot sample extracts. In contrasts, BEQ values for dioxin like compounds from remote regions were much lower and occasionally below the quantification limits of the method. The BEQ activities obtained in this study have a similar trend to the WHO-TEQ results for the samples collected in the A-So airbase. However, BEQ values were higher than those of TEQ, probably reflecting the presence of additional AhR ligands and/or possible non-additive interactions in the sample mixture. This study confirms that after more than 60 years, a strong residual pollution of PCDD/Fs remains on this former air base following the use and storage of Agent Orange during the Vietnam War, raising a health risk for populations exposed in this area because livestock animals graze there. |
topic |
CALUX bioassay PCDD/Fs Dioxin-like PCBs A-So former airbase |
url |
http://www.sciencedirect.com/science/article/pii/S0147651321000828 |
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