Semi-solid detergent agar: an alternative for the conservation ex situ of Cladosporium spp. (Ascomycota: Cladosporiaceae)
The genus Cladosporium is ubiquitous and has a broad environmental representation. For the conservation of strains of this fungal genus, several methods of choice have been used, among which are cryopreservation and lyophilization. However, other alternative and complementary methods reduce conserva...
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doaj-064a67625c7c4e9fb290f272a7b1efa32021-09-04T15:23:27ZengUniversidad de La HabanaRevista del Jardín Botánico Nacional0253-56962410-55462021-08-01420203207564Semi-solid detergent agar: an alternative for the conservation ex situ of Cladosporium spp. (Ascomycota: Cladosporiaceae)Ian Pérez Ramírez0Kenia Caridad Sánchez Espinosa1Teresa Irene Rojas Flores2Michel Almaguer Chávez3Departamento de Microbiología, Facultad de Biología, Universidad de La Habana, Calle 25, Nº 455, e/ I y J, Plaza de la Revolución, La Habana, Cuba. C.P. 10400Departamento de Microbiología, Facultad de Biología, Universidad de La Habana, Calle 25, Nº 455, e/ I y J, Plaza de la Revolución, La Habana, Cuba. C.P. 10400Departamento de Microbiología, Facultad de Biología, Universidad de La Habana, Calle 25, Nº 455, e/ I y J, Plaza de la Revolución, La Habana, Cuba. C.P. 10400Departamento de Microbiología, Facultad de Biología, Universidad de La Habana, Calle 25, Nº 455, e/ I y J, Plaza de la Revolución, La Habana, Cuba. C.P. 10400The genus Cladosporium is ubiquitous and has a broad environmental representation. For the conservation of strains of this fungal genus, several methods of choice have been used, among which are cryopreservation and lyophilization. However, other alternative and complementary methods reduce conservation costs. The purpose of this investigation was to evaluate the feasibility of the conservation of 92 strains of Cladosporium in the medium Semisolid Detergent Agar (2 % bacteriological agar, 0.05 % Tween 80 and 100 ml of sterile distilled water). Viability, purity and morphological integrity were determined up to three years after initial conservation. For conservation, 1.5 ml Eppendorf™ tubes with 1 ml of medium were used, which were inoculated with spores obtained from a 7-day culture in Papa Dextrose Agar and kept at 4 °C. During the evaluated period, the strains maintained viability with values higher than 90 %, so this can be an alternative method for the conservation of strains of Cladosporium in the short or medium term. Citation: Pérez, I., Sánchez, K.C., Rojas, T.I. & Almaguer, M. 2021. Agar detergente semisólido: una alternativa para la conservación de cepas de Cladosporium. Revista Jard. Bot. Nac. Univ. Habana 42: 203-207. Received: 13 August 2020. Accepted: 23 December 2020. Online: 13 August 2021. Editor: Milay Cabarroi.http://www.rjbn.uh.cu/index.php/RJBN/article/view/563cultivos microbianoshongopreservación de microorganismosviabilidad |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ian Pérez Ramírez Kenia Caridad Sánchez Espinosa Teresa Irene Rojas Flores Michel Almaguer Chávez |
spellingShingle |
Ian Pérez Ramírez Kenia Caridad Sánchez Espinosa Teresa Irene Rojas Flores Michel Almaguer Chávez Semi-solid detergent agar: an alternative for the conservation ex situ of Cladosporium spp. (Ascomycota: Cladosporiaceae) Revista del Jardín Botánico Nacional cultivos microbianos hongo preservación de microorganismos viabilidad |
author_facet |
Ian Pérez Ramírez Kenia Caridad Sánchez Espinosa Teresa Irene Rojas Flores Michel Almaguer Chávez |
author_sort |
Ian Pérez Ramírez |
title |
Semi-solid detergent agar: an alternative for the conservation ex situ of Cladosporium spp. (Ascomycota: Cladosporiaceae) |
title_short |
Semi-solid detergent agar: an alternative for the conservation ex situ of Cladosporium spp. (Ascomycota: Cladosporiaceae) |
title_full |
Semi-solid detergent agar: an alternative for the conservation ex situ of Cladosporium spp. (Ascomycota: Cladosporiaceae) |
title_fullStr |
Semi-solid detergent agar: an alternative for the conservation ex situ of Cladosporium spp. (Ascomycota: Cladosporiaceae) |
title_full_unstemmed |
Semi-solid detergent agar: an alternative for the conservation ex situ of Cladosporium spp. (Ascomycota: Cladosporiaceae) |
title_sort |
semi-solid detergent agar: an alternative for the conservation ex situ of cladosporium spp. (ascomycota: cladosporiaceae) |
publisher |
Universidad de La Habana |
series |
Revista del Jardín Botánico Nacional |
issn |
0253-5696 2410-5546 |
publishDate |
2021-08-01 |
description |
The genus Cladosporium is ubiquitous and has a broad environmental representation. For the conservation of strains of this fungal genus, several methods of choice have been used, among which are cryopreservation and lyophilization. However, other alternative and complementary methods reduce conservation costs. The purpose of this investigation was to evaluate the feasibility of the conservation of 92 strains of Cladosporium in the medium Semisolid Detergent Agar (2 % bacteriological agar, 0.05 % Tween 80 and 100 ml of sterile distilled water). Viability, purity and morphological integrity were determined up to three years after initial conservation. For conservation, 1.5 ml Eppendorf™ tubes with 1 ml of medium were used, which were inoculated with spores obtained from a 7-day culture in Papa Dextrose Agar and kept at 4 °C. During the evaluated period, the strains maintained viability with values higher than 90 %, so this can be an alternative method for the conservation of strains of Cladosporium in the short or medium term.
Citation: Pérez, I., Sánchez, K.C., Rojas, T.I. & Almaguer, M. 2021. Agar detergente semisólido: una alternativa para la conservación de cepas de Cladosporium. Revista Jard. Bot. Nac. Univ. Habana 42: 203-207.
Received: 13 August 2020. Accepted: 23 December 2020. Online: 13 August 2021. Editor: Milay Cabarroi. |
topic |
cultivos microbianos hongo preservación de microorganismos viabilidad |
url |
http://www.rjbn.uh.cu/index.php/RJBN/article/view/563 |
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