Differential analysis of quantitative proteome and acetyl-proteome profiling between premenopausal and postmenopausal ovarian tissues
Abstract Background Natural menopause is always accompanied by specific signs and symptoms, suggesting physiological changes in this peoriod. However, no systematic study has assessed the changes at molecular level in the ovaries during the menopausal transition so far. This study integrated quantit...
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doaj-06551117832b4da4b5f37ed11e545d0d2020-11-24T22:20:02ZengBMCClinical Proteomics1542-64161559-02752018-11-0115111210.1186/s12014-018-9214-0Differential analysis of quantitative proteome and acetyl-proteome profiling between premenopausal and postmenopausal ovarian tissuesJinling Yi0Huatianshu Hu1Peipei Shi2Song Shi3Junda Zhao4Linna Xu5Weining Yang6Bin Li7Jin Zhu8Shien Zou9Department of Gynecology, The Fifth Affiliated Hospital of Xin Jiang Medical UniversityDepartment of Gynecology, Obstetrics and Gynecology Hospital, Fudan UniversityDepartment of Gynecology, Obstetrics and Gynecology Hospital, Fudan UniversityDepartment of Function Inspection, The Fifth Affiliated Hospital of Xin Jiang Medical UniversityDepartment of Gynecology, The First Affiliated Hospital of Xinjiang Medical UniversityDepartment of Gynecology, Obstetrics and Gynecology Hospital, Fudan UniversityDepartment of Gynecology, Obstetrics and Gynecology Hospital, Fudan UniversityDepartment of Gynecology, Obstetrics and Gynecology Hospital, Fudan UniversityDepartment of Gynecology, Obstetrics and Gynecology Hospital, Fudan UniversityDepartment of Gynecology, Obstetrics and Gynecology Hospital, Fudan UniversityAbstract Background Natural menopause is always accompanied by specific signs and symptoms, suggesting physiological changes in this peoriod. However, no systematic study has assessed the changes at molecular level in the ovaries during the menopausal transition so far. This study integrated quantitative proteome and acetyl-proteome to comprehensively uncover the changes of ovarian protein and protein-acetylation profiles in this transitional period. The findings would provide novel insights into the biology of menopause and help relieve and treat the associated signs and symptoms, further improving the women’s health care. Methods Freshly thawed ovarian tissue samples obtained from premenopausal and postmenopausal women were assessed with Tandem Mass Tags for the quantitative analysis of the global profile and acetyl-proteomes by 2-dimensional separation and LC–MS/MS. Results Comprehensively, 4210 types of protein, with 3551 types quantifiable were detected. 3047 acetylated sites in 1583 types of protein with 2256 quantifiable in 1248 proteins were detected. By comparing the global and acetylated proteome profiles for postmenopausal women and premenopausal women, 151 types of proteins were found upregulated and 65 were downregulated, along with 23 acetylated sites upregulated and 220 sites downregulated. For Immune response, the complement and coagulation cascades plus the citrate cycle and cellular detoxification were found to be significantly enhanced, while the extracellular structure and matrix organization, ECM-receptor interactions plus the infections were markedly suppressed. In addition, the amino acids around the acetylated sites were enriched by motif analysis, which can help us uncover amino acid sequence and search for the specific target in the subsequent study. Conclusion Global and acetylated proteome Profiles in ovary differ between the premenopausal and postmenopausal groups. These proteomic-level changes may offer some potential biological markers to identify the pathological changes in ovary and help relieve and treat the associated signs and symptoms, and ultimately improve women’s health care.http://link.springer.com/article/10.1186/s12014-018-9214-0Human ovarianMenopauseProteomeAcetyl-proteome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jinling Yi Huatianshu Hu Peipei Shi Song Shi Junda Zhao Linna Xu Weining Yang Bin Li Jin Zhu Shien Zou |
spellingShingle |
Jinling Yi Huatianshu Hu Peipei Shi Song Shi Junda Zhao Linna Xu Weining Yang Bin Li Jin Zhu Shien Zou Differential analysis of quantitative proteome and acetyl-proteome profiling between premenopausal and postmenopausal ovarian tissues Clinical Proteomics Human ovarian Menopause Proteome Acetyl-proteome |
author_facet |
Jinling Yi Huatianshu Hu Peipei Shi Song Shi Junda Zhao Linna Xu Weining Yang Bin Li Jin Zhu Shien Zou |
author_sort |
Jinling Yi |
title |
Differential analysis of quantitative proteome and acetyl-proteome profiling between premenopausal and postmenopausal ovarian tissues |
title_short |
Differential analysis of quantitative proteome and acetyl-proteome profiling between premenopausal and postmenopausal ovarian tissues |
title_full |
Differential analysis of quantitative proteome and acetyl-proteome profiling between premenopausal and postmenopausal ovarian tissues |
title_fullStr |
Differential analysis of quantitative proteome and acetyl-proteome profiling between premenopausal and postmenopausal ovarian tissues |
title_full_unstemmed |
Differential analysis of quantitative proteome and acetyl-proteome profiling between premenopausal and postmenopausal ovarian tissues |
title_sort |
differential analysis of quantitative proteome and acetyl-proteome profiling between premenopausal and postmenopausal ovarian tissues |
publisher |
BMC |
series |
Clinical Proteomics |
issn |
1542-6416 1559-0275 |
publishDate |
2018-11-01 |
description |
Abstract Background Natural menopause is always accompanied by specific signs and symptoms, suggesting physiological changes in this peoriod. However, no systematic study has assessed the changes at molecular level in the ovaries during the menopausal transition so far. This study integrated quantitative proteome and acetyl-proteome to comprehensively uncover the changes of ovarian protein and protein-acetylation profiles in this transitional period. The findings would provide novel insights into the biology of menopause and help relieve and treat the associated signs and symptoms, further improving the women’s health care. Methods Freshly thawed ovarian tissue samples obtained from premenopausal and postmenopausal women were assessed with Tandem Mass Tags for the quantitative analysis of the global profile and acetyl-proteomes by 2-dimensional separation and LC–MS/MS. Results Comprehensively, 4210 types of protein, with 3551 types quantifiable were detected. 3047 acetylated sites in 1583 types of protein with 2256 quantifiable in 1248 proteins were detected. By comparing the global and acetylated proteome profiles for postmenopausal women and premenopausal women, 151 types of proteins were found upregulated and 65 were downregulated, along with 23 acetylated sites upregulated and 220 sites downregulated. For Immune response, the complement and coagulation cascades plus the citrate cycle and cellular detoxification were found to be significantly enhanced, while the extracellular structure and matrix organization, ECM-receptor interactions plus the infections were markedly suppressed. In addition, the amino acids around the acetylated sites were enriched by motif analysis, which can help us uncover amino acid sequence and search for the specific target in the subsequent study. Conclusion Global and acetylated proteome Profiles in ovary differ between the premenopausal and postmenopausal groups. These proteomic-level changes may offer some potential biological markers to identify the pathological changes in ovary and help relieve and treat the associated signs and symptoms, and ultimately improve women’s health care. |
topic |
Human ovarian Menopause Proteome Acetyl-proteome |
url |
http://link.springer.com/article/10.1186/s12014-018-9214-0 |
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