Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats
POU (Pit-Oct-Unc) class 1 homeobox 1 (<i>POU1F1</i>, or <i>Pit-1</i>) is a transcription factor that directly regulates pituitary hormone-related genes, as well as affects the reproduction and growth in mammals. Thus, <i>POU1F1</i> gene was investigated as a candi...
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doaj-065910d9526c4b1ea4aa42b86d847c992020-11-24T20:41:56ZengMDPI AGAnimals2076-26152019-03-019311410.3390/ani9030114ani9030114Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere GoatsHaijing Zhu0Yanghai Zhang1Yangyang Bai2Han Yang3Hailong Yan4Jinwang Liu5Lei Shi6Xiaoyue Song7Longping Li8Shuwei Dong9Chuanying Pan10Xianyong Lan11Lei Qu12Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaPOU (Pit-Oct-Unc) class 1 homeobox 1 (<i>POU1F1</i>, or <i>Pit-1</i>) is a transcription factor that directly regulates pituitary hormone-related genes, as well as affects the reproduction and growth in mammals. Thus, <i>POU1F1</i> gene was investigated as a candidate gene for litter size and growth performance in goats. In the current study, using direct DNA sequencing, c.682G > T, c.723T > G and c.837T > C loci were genotyped in Shaanbei white cashmere (SBWC) goats (n = 609), but c.876 + 110T > C was monomorphic. Besides, the c.682G > T locus was first identified by <i>Hinf</i>I (<i>Haemophilus influenzae</i> Rf) restriction endonuclease. Association analysis results showed that the c.682G > T, c.837T > C loci and diplotypes were significantly associated with goat litter size (<i>p</i> < 0.05). The positive genotypes were GT and TT for the two SNPs, respectively, and the optimal diplotype was H3H7 (GTTT-TTTT). On the other hand, the c.682G > T, c.723T > G and c.837T > C strongly affected growth traits and body measurement indexes in SBWC goats (<i>p</i> < 0.05). The positive genotypes or allele of these SNPs were GT, G and TT, respectively. Additionally, the goats with H3H7 diplotype also had a greater growth status than others (<i>p</i> < 0.05). Here, individuals with same genotype had both a better litter size and growth traits, showing a positive correlation between these economic traits. Meanwhile, the positive genotypes of four SNPs were combined to obtain the optimal diplotype, which was also H3H7. These SNPs, especially the diplotype, could be used for the genomic selection of excellent individuals with a greater litter size and better growth status in goat breeding.https://www.mdpi.com/2076-2615/9/3/114goatPOU class 1 homeobox 1 genesingle nucleotide polymorphismsdiplotypereproductiongrowth traits |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Haijing Zhu Yanghai Zhang Yangyang Bai Han Yang Hailong Yan Jinwang Liu Lei Shi Xiaoyue Song Longping Li Shuwei Dong Chuanying Pan Xianyong Lan Lei Qu |
spellingShingle |
Haijing Zhu Yanghai Zhang Yangyang Bai Han Yang Hailong Yan Jinwang Liu Lei Shi Xiaoyue Song Longping Li Shuwei Dong Chuanying Pan Xianyong Lan Lei Qu Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats Animals goat POU class 1 homeobox 1 gene single nucleotide polymorphisms diplotype reproduction growth traits |
author_facet |
Haijing Zhu Yanghai Zhang Yangyang Bai Han Yang Hailong Yan Jinwang Liu Lei Shi Xiaoyue Song Longping Li Shuwei Dong Chuanying Pan Xianyong Lan Lei Qu |
author_sort |
Haijing Zhu |
title |
Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats |
title_short |
Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats |
title_full |
Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats |
title_fullStr |
Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats |
title_full_unstemmed |
Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats |
title_sort |
relationship between snps of <i>pou1f1</i> gene and litter size and growth traits in shaanbei white cashmere goats |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2019-03-01 |
description |
POU (Pit-Oct-Unc) class 1 homeobox 1 (<i>POU1F1</i>, or <i>Pit-1</i>) is a transcription factor that directly regulates pituitary hormone-related genes, as well as affects the reproduction and growth in mammals. Thus, <i>POU1F1</i> gene was investigated as a candidate gene for litter size and growth performance in goats. In the current study, using direct DNA sequencing, c.682G > T, c.723T > G and c.837T > C loci were genotyped in Shaanbei white cashmere (SBWC) goats (n = 609), but c.876 + 110T > C was monomorphic. Besides, the c.682G > T locus was first identified by <i>Hinf</i>I (<i>Haemophilus influenzae</i> Rf) restriction endonuclease. Association analysis results showed that the c.682G > T, c.837T > C loci and diplotypes were significantly associated with goat litter size (<i>p</i> < 0.05). The positive genotypes were GT and TT for the two SNPs, respectively, and the optimal diplotype was H3H7 (GTTT-TTTT). On the other hand, the c.682G > T, c.723T > G and c.837T > C strongly affected growth traits and body measurement indexes in SBWC goats (<i>p</i> < 0.05). The positive genotypes or allele of these SNPs were GT, G and TT, respectively. Additionally, the goats with H3H7 diplotype also had a greater growth status than others (<i>p</i> < 0.05). Here, individuals with same genotype had both a better litter size and growth traits, showing a positive correlation between these economic traits. Meanwhile, the positive genotypes of four SNPs were combined to obtain the optimal diplotype, which was also H3H7. These SNPs, especially the diplotype, could be used for the genomic selection of excellent individuals with a greater litter size and better growth status in goat breeding. |
topic |
goat POU class 1 homeobox 1 gene single nucleotide polymorphisms diplotype reproduction growth traits |
url |
https://www.mdpi.com/2076-2615/9/3/114 |
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