Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats

Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats

POU (Pit-Oct-Unc) class 1 homeobox 1 (<i>POU1F1</i>, or <i>Pit-1</i>) is a transcription factor that directly regulates pituitary hormone-related genes, as well as affects the reproduction and growth in mammals. Thus, <i>POU1F1</i> gene was investigated as a candi...

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Main Authors: Haijing Zhu, Yanghai Zhang, Yangyang Bai, Han Yang, Hailong Yan, Jinwang Liu, Lei Shi, Xiaoyue Song, Longping Li, Shuwei Dong, Chuanying Pan, Xianyong Lan, Lei Qu
Format: Article
Language:English
Published: MDPI AG 2019-03-01
Series:Animals
Subjects:
goat
POU class 1 homeobox 1 gene
single nucleotide polymorphisms
diplotype
reproduction
growth traits
Online Access:https://www.mdpi.com/2076-2615/9/3/114
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spelling doaj-065910d9526c4b1ea4aa42b86d847c992020-11-24T20:41:56ZengMDPI AGAnimals2076-26152019-03-019311410.3390/ani9030114ani9030114Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere GoatsHaijing Zhu0Yanghai Zhang1Yangyang Bai2Han Yang3Hailong Yan4Jinwang Liu5Lei Shi6Xiaoyue Song7Longping Li8Shuwei Dong9Chuanying Pan10Xianyong Lan11Lei Qu12Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&amp;F University, Yangling 712100, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&amp;F University, Yangling 712100, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&amp;F University, Yangling 712100, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&amp;F University, Yangling 712100, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, ChinaPOU (Pit-Oct-Unc) class 1 homeobox 1 (<i>POU1F1</i>, or <i>Pit-1</i>) is a transcription factor that directly regulates pituitary hormone-related genes, as well as affects the reproduction and growth in mammals. Thus, <i>POU1F1</i> gene was investigated as a candidate gene for litter size and growth performance in goats. In the current study, using direct DNA sequencing, c.682G &gt; T, c.723T &gt; G and c.837T &gt; C loci were genotyped in Shaanbei white cashmere (SBWC) goats (n = 609), but c.876 + 110T &gt; C was monomorphic. Besides, the c.682G &gt; T locus was first identified by <i>Hinf</i>I (<i>Haemophilus influenzae</i> Rf) restriction endonuclease. Association analysis results showed that the c.682G &gt; T, c.837T &gt; C loci and diplotypes were significantly associated with goat litter size (<i>p</i> &lt; 0.05). The positive genotypes were GT and TT for the two SNPs, respectively, and the optimal diplotype was H3H7 (GTTT-TTTT). On the other hand, the c.682G &gt; T, c.723T &gt; G and c.837T &gt; C strongly affected growth traits and body measurement indexes in SBWC goats (<i>p</i> &lt; 0.05). The positive genotypes or allele of these SNPs were GT, G and TT, respectively. Additionally, the goats with H3H7 diplotype also had a greater growth status than others (<i>p</i> &lt; 0.05). Here, individuals with same genotype had both a better litter size and growth traits, showing a positive correlation between these economic traits. Meanwhile, the positive genotypes of four SNPs were combined to obtain the optimal diplotype, which was also H3H7. These SNPs, especially the diplotype, could be used for the genomic selection of excellent individuals with a greater litter size and better growth status in goat breeding.https://www.mdpi.com/2076-2615/9/3/114goatPOU class 1 homeobox 1 genesingle nucleotide polymorphismsdiplotypereproductiongrowth traits
collection DOAJ
language English
format Article
sources DOAJ
author Haijing Zhu
Yanghai Zhang
Yangyang Bai
Han Yang
Hailong Yan
Jinwang Liu
Lei Shi
Xiaoyue Song
Longping Li
Shuwei Dong
Chuanying Pan
Xianyong Lan
Lei Qu
spellingShingle Haijing Zhu
Yanghai Zhang
Yangyang Bai
Han Yang
Hailong Yan
Jinwang Liu
Lei Shi
Xiaoyue Song
Longping Li
Shuwei Dong
Chuanying Pan
Xianyong Lan
Lei Qu
Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats
Animals
goat
POU class 1 homeobox 1 gene
single nucleotide polymorphisms
diplotype
reproduction
growth traits
author_facet Haijing Zhu
Yanghai Zhang
Yangyang Bai
Han Yang
Hailong Yan
Jinwang Liu
Lei Shi
Xiaoyue Song
Longping Li
Shuwei Dong
Chuanying Pan
Xianyong Lan
Lei Qu
author_sort Haijing Zhu
title Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats
title_short Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats
title_full Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats
title_fullStr Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats
title_full_unstemmed Relationship between SNPs of <i>POU1F1</i> Gene and Litter Size and Growth Traits in Shaanbei White Cashmere Goats
title_sort relationship between snps of <i>pou1f1</i> gene and litter size and growth traits in shaanbei white cashmere goats
publisher MDPI AG
series Animals
issn 2076-2615
publishDate 2019-03-01
description POU (Pit-Oct-Unc) class 1 homeobox 1 (<i>POU1F1</i>, or <i>Pit-1</i>) is a transcription factor that directly regulates pituitary hormone-related genes, as well as affects the reproduction and growth in mammals. Thus, <i>POU1F1</i> gene was investigated as a candidate gene for litter size and growth performance in goats. In the current study, using direct DNA sequencing, c.682G &gt; T, c.723T &gt; G and c.837T &gt; C loci were genotyped in Shaanbei white cashmere (SBWC) goats (n = 609), but c.876 + 110T &gt; C was monomorphic. Besides, the c.682G &gt; T locus was first identified by <i>Hinf</i>I (<i>Haemophilus influenzae</i> Rf) restriction endonuclease. Association analysis results showed that the c.682G &gt; T, c.837T &gt; C loci and diplotypes were significantly associated with goat litter size (<i>p</i> &lt; 0.05). The positive genotypes were GT and TT for the two SNPs, respectively, and the optimal diplotype was H3H7 (GTTT-TTTT). On the other hand, the c.682G &gt; T, c.723T &gt; G and c.837T &gt; C strongly affected growth traits and body measurement indexes in SBWC goats (<i>p</i> &lt; 0.05). The positive genotypes or allele of these SNPs were GT, G and TT, respectively. Additionally, the goats with H3H7 diplotype also had a greater growth status than others (<i>p</i> &lt; 0.05). Here, individuals with same genotype had both a better litter size and growth traits, showing a positive correlation between these economic traits. Meanwhile, the positive genotypes of four SNPs were combined to obtain the optimal diplotype, which was also H3H7. These SNPs, especially the diplotype, could be used for the genomic selection of excellent individuals with a greater litter size and better growth status in goat breeding.
topic goat
POU class 1 homeobox 1 gene
single nucleotide polymorphisms
diplotype
reproduction
growth traits
url https://www.mdpi.com/2076-2615/9/3/114
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