Preparation of Metabolic Antigen from Aspergillus fumigatus for Serodiagnosis of Aspergillosis

• Main Authors: Seyyed Hossein Mirhendi, Seyyed Amir Ghiasian
• Format: Article
• Language: fas
• Published: Hamadan University of Medical Sciences 2002-03-01
• Series: پزشکی بالینی ابن سینا
• Subjects: : aspergillosis / aspergillus fumigatus / metabolic antigen
• Online Access: http://sjh.umsha.ac.ir/article-1-799-en.html

Aspergillosis is an important systemic fungal infection with various clinical    forms and its clinical and mycological diagnosis is difficult and    compelicated but its serodiagnosis is simple and useful for some clinical    forms (aspergillomma and allergic aspergillosis). This study was performed for    preparing metabolic antigen and related antiserum of the most important    agent of aspergillosis, “Aspergillus fumigatus", for using in serodiagnosing the    disease.           The fungus was isolated from the patients, and cultured in glucose    peptone broth for 5 weeks. After removing the fungal mass, the secretary    (metabolic) antigens was isolated and processed and injected to rabbit.    Antisera were evaluated by double- diffusion (DD).           Prepared antigen was able to immunize rabbits and reacted with rabbit    and patient’s sera in immunodiffusion. Number and pattern of the bands    was comparable with standard antigen and antiserum.           After standardization, this antigen, it can be a useful reagent to    serodiagnosis of aspergillosis by lower cost and more accesibility.