| Summary: | The increasing spatial and temporal scales of ecological recovery projects demand more rapid and accurate methods of predicting restoration trajectory. Unmanned aerial vehicles (UAVs) offer greatly improved rapidity and efficiency compared to traditional biodiversity monitoring surveys and are increasingly employed in the monitoring of ecological restoration. However, the applicability of UAV-based remote sensing in the identification of small features of interest from captured imagery (e.g., small individual plants, <100 cm<sup>2</sup>) remains untested and the potential of UAVs to track the performance of individual plants or the development of seedlings remains unexplored. This study utilised low-altitude UAV imagery from multi-sensor flights (Red-Green-Blue and multispectral sensors) and an automated object-based image analysis software to detect target seedlings from among a matrix of non-target grasses in order to track the performance of individual target seedlings and the seedling community over a 14-week period. Object-based Image Analysis (OBIA) classification effectively and accurately discriminated among target and non-target seedling objects and these groups exhibited distinct spectral signatures (six different visible-spectrum and multispectral indices) that responded differently over a 24-day drying period. OBIA classification from captured imagery also allowed for the accurate tracking of individual target seedling objects through time, clearly illustrating the capacity of UAV-based monitoring to undertake plant performance monitoring of individual plants at very fine spatial scales.
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