Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic
Background: Cellulite is a condition in which the skin has a dimpled lumpy appearance. The main causes of cellulite development, studied until now, comprehends modified sensitivity to estrogens, the damage of microvasculature present among dermis and hypodermis. The differences of adipose tissue arc...
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Language: | English |
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MDPI AG
2020-03-01
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Series: | International Journal of Molecular Sciences |
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Online Access: | https://www.mdpi.com/1422-0067/21/6/2077 |
id |
doaj-06938885c2e247838983be7a69fd0f01 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Giamaica Conti Nicola Zingaretti Domenico Amuso Elena Dai Prè Jessica Brandi Daniela Cecconi Marcello Manfredi Emilio Marengo Federico Boschi Michele Riccio Roberto Amore Eugenio Luigi Iorio Alice Busato Francesco De Francesco Valentina Riccio Pier Camillo Parodi Luca Vaienti Andrea Sbarbati |
spellingShingle |
Giamaica Conti Nicola Zingaretti Domenico Amuso Elena Dai Prè Jessica Brandi Daniela Cecconi Marcello Manfredi Emilio Marengo Federico Boschi Michele Riccio Roberto Amore Eugenio Luigi Iorio Alice Busato Francesco De Francesco Valentina Riccio Pier Camillo Parodi Luca Vaienti Andrea Sbarbati Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic International Journal of Molecular Sciences adult stem cells mesenchymal stem cell cellulite proteomic analysis dermal adipose tissue |
author_facet |
Giamaica Conti Nicola Zingaretti Domenico Amuso Elena Dai Prè Jessica Brandi Daniela Cecconi Marcello Manfredi Emilio Marengo Federico Boschi Michele Riccio Roberto Amore Eugenio Luigi Iorio Alice Busato Francesco De Francesco Valentina Riccio Pier Camillo Parodi Luca Vaienti Andrea Sbarbati |
author_sort |
Giamaica Conti |
title |
Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic |
title_short |
Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic |
title_full |
Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic |
title_fullStr |
Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic |
title_full_unstemmed |
Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic |
title_sort |
proteomic and ultrastructural analysis of cellulite—new findings on an old topic |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-03-01 |
description |
Background: Cellulite is a condition in which the skin has a dimpled lumpy appearance. The main causes of cellulite development, studied until now, comprehends modified sensitivity to estrogens, the damage of microvasculature present among dermis and hypodermis. The differences of adipose tissue architecture between male and female might make female more susceptible to cellulite. Adipose tissue is seen to be deeply modified during cellulite development. Our study tried to understand the overall features within and surrounding cellulite to apply the best therapeutic approach. Methods: Samples of gluteal femoral area were collected from cadavers and women who had undergone surgical treatment to remove orange peel characteristics on the skin. Samples from cadavers were employed for an accurate study of cellulite using magnetic resonance imaging at 7 Tesla and for light microscopy. Specimens from patients were employed for the proteomic analysis, which was performed using high resolution mass spectroscopy (MS). Stromal vascular fraction (SVF) was obtained from the samples, which was studied using MS and flow cytometry. Results: light and electron microscopy of the cellulite affected area showed a morphology completely different from the other usual adipose depots. In cellulite affected tissues, sweat glands associated with adipocytes were found. In particular, there were vesicles in the extracellular matrix, indicating a crosstalk between the two different components. Proteomic analysis showed that adipose tissue affected by cellulite is characterized by high degree of oxidative stress and by remodeling phenomena. Conclusions: The novel aspects of this study are the peculiar morphology of adipose tissue affected by cellulite, which could influence the surgical procedures finalized to the reduction of dimpling, based on the collagen fibers cutting. The second novel aspect is the role played by the mesenchymal stem cells isolated from stromal vascular fraction of adipose tissue affected by cellulite. |
topic |
adult stem cells mesenchymal stem cell cellulite proteomic analysis dermal adipose tissue |
url |
https://www.mdpi.com/1422-0067/21/6/2077 |
work_keys_str_mv |
AT giamaicaconti proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT nicolazingaretti proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT domenicoamuso proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT elenadaipre proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT jessicabrandi proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT danielacecconi proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT marcellomanfredi proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT emiliomarengo proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT federicoboschi proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT michelericcio proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT robertoamore proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT eugenioluigiiorio proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT alicebusato proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT francescodefrancesco proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT valentinariccio proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT piercamilloparodi proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT lucavaienti proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic AT andreasbarbati proteomicandultrastructuralanalysisofcellulitenewfindingsonanoldtopic |
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doaj-06938885c2e247838983be7a69fd0f012020-11-25T03:29:28ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-03-01216207710.3390/ijms21062077ijms21062077Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old TopicGiamaica Conti0Nicola Zingaretti1Domenico Amuso2Elena Dai Prè3Jessica Brandi4Daniela Cecconi5Marcello Manfredi6Emilio Marengo7Federico Boschi8Michele Riccio9Roberto Amore10Eugenio Luigi Iorio11Alice Busato12Francesco De Francesco13Valentina Riccio14Pier Camillo Parodi15Luca Vaienti16Andrea Sbarbati17Department of Neurosciences, Biomedicine and Movement Sciences, Anatomy and Histology division, University of Verona, 37134 Verona, ItalyAccademia del Lipofilling, Research and Training Center in Regenerative Surgery, 61025 Montelabbate, ItalyMaster of Aesthetic Regenerative and Anti-Aging Medicine, University of Verona, 37134 Verona, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, Anatomy and Histology division, University of Verona, 37134 Verona, ItalyDepartment of Biochemistry, University of Verona, 37134 Verona, ItalyDepartment of Biochemistry, University of Verona, 37134 Verona, ItalyDepartment of Sciences and Technological Innovation, University of Eastern Piedmont, 15121 Alessandria, ItalyDepartment of Sciences and Technological Innovation, University of Eastern Piedmont, 15121 Alessandria, ItalyDepartment of Informatics, University of Verona, 37134 Verona, ItalyAccademia del Lipofilling, Research and Training Center in Regenerative Surgery, 61025 Montelabbate, ItalyMaster of Aesthetic Regenerative and Anti-Aging Medicine, University of Verona, 37134 Verona, ItalyMaster of Aesthetic Regenerative and Anti-Aging Medicine, University of Verona, 37134 Verona, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, Anatomy and Histology division, University of Verona, 37134 Verona, ItalyAccademia del Lipofilling, Research and Training Center in Regenerative Surgery, 61025 Montelabbate, ItalyAccademia del Lipofilling, Research and Training Center in Regenerative Surgery, 61025 Montelabbate, ItalyAccademia del Lipofilling, Research and Training Center in Regenerative Surgery, 61025 Montelabbate, ItalyAccademia del Lipofilling, Research and Training Center in Regenerative Surgery, 61025 Montelabbate, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, Anatomy and Histology division, University of Verona, 37134 Verona, ItalyBackground: Cellulite is a condition in which the skin has a dimpled lumpy appearance. The main causes of cellulite development, studied until now, comprehends modified sensitivity to estrogens, the damage of microvasculature present among dermis and hypodermis. The differences of adipose tissue architecture between male and female might make female more susceptible to cellulite. Adipose tissue is seen to be deeply modified during cellulite development. Our study tried to understand the overall features within and surrounding cellulite to apply the best therapeutic approach. Methods: Samples of gluteal femoral area were collected from cadavers and women who had undergone surgical treatment to remove orange peel characteristics on the skin. Samples from cadavers were employed for an accurate study of cellulite using magnetic resonance imaging at 7 Tesla and for light microscopy. Specimens from patients were employed for the proteomic analysis, which was performed using high resolution mass spectroscopy (MS). Stromal vascular fraction (SVF) was obtained from the samples, which was studied using MS and flow cytometry. Results: light and electron microscopy of the cellulite affected area showed a morphology completely different from the other usual adipose depots. In cellulite affected tissues, sweat glands associated with adipocytes were found. In particular, there were vesicles in the extracellular matrix, indicating a crosstalk between the two different components. Proteomic analysis showed that adipose tissue affected by cellulite is characterized by high degree of oxidative stress and by remodeling phenomena. Conclusions: The novel aspects of this study are the peculiar morphology of adipose tissue affected by cellulite, which could influence the surgical procedures finalized to the reduction of dimpling, based on the collagen fibers cutting. The second novel aspect is the role played by the mesenchymal stem cells isolated from stromal vascular fraction of adipose tissue affected by cellulite.https://www.mdpi.com/1422-0067/21/6/2077adult stem cellsmesenchymal stem cellcelluliteproteomic analysisdermal adipose tissue |