Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists
Fusarium graminearum is the main causal pathogen affecting small-grain cereals, and it produces deoxynivalenol, a kind of mycotoxin, which displays a wide range of toxic effects in human and animals. Bacterial strains isolated from peanut shells were investigated for their activities against F. gram...
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doaj-06a0ad40d83a4efa99a9dbac87552dd32020-11-25T02:27:13ZengMDPI AGInternational Journal of Environmental Research and Public Health1660-46012014-01-011111094110510.3390/ijerph110101094ijerph110101094Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial AntagonistsCuijuan Shi0Peisheng Yan1Jiafei Li2Hanqi Wu3Qianwei Li4Shanshan Guan5School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, ChinaSchool of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, ChinaSchool of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, ChinaSchool of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, ChinaSchool of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, ChinaSchool of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, ChinaFusarium graminearum is the main causal pathogen affecting small-grain cereals, and it produces deoxynivalenol, a kind of mycotoxin, which displays a wide range of toxic effects in human and animals. Bacterial strains isolated from peanut shells were investigated for their activities against F. graminearum by dual-culture plate and tip-culture assays. Among them, twenty strains exhibited potent inhibition to the growth of F. graminearum, and the inhibition rates ranged from 41.41% to 54.55% in dual-culture plate assay and 92.70% to 100% in tip-culture assay. Furthermore, eighteen strains reduced the production of deoxynivalenol by 16.69% to 90.30% in the wheat kernels assay. Finally, the strains with the strongest inhibitory activity were identified by morphological, physiological, biochemical methods and also 16S rDNA and gyrA gene analysis as Bacillus amyloliquefaciens. The current study highlights the potential application of antagonistic microorganisms and their metabolites in the prevention of fungal growth and mycotoxin production in wheat kernels. As a biological strategy, it might avoid safety problems and nutrition loss which always caused by physical and chemical strategies.http://www.mdpi.com/1660-4601/11/1/1094bacterial antagonistsbiocontrol deoxynivalenolFusarium graminearumwheat kernels |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cuijuan Shi Peisheng Yan Jiafei Li Hanqi Wu Qianwei Li Shanshan Guan |
spellingShingle |
Cuijuan Shi Peisheng Yan Jiafei Li Hanqi Wu Qianwei Li Shanshan Guan Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists International Journal of Environmental Research and Public Health bacterial antagonists biocontrol deoxynivalenol Fusarium graminearum wheat kernels |
author_facet |
Cuijuan Shi Peisheng Yan Jiafei Li Hanqi Wu Qianwei Li Shanshan Guan |
author_sort |
Cuijuan Shi |
title |
Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists |
title_short |
Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists |
title_full |
Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists |
title_fullStr |
Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists |
title_full_unstemmed |
Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists |
title_sort |
biocontrol of fusarium graminearum growth and deoxynivalenol production in wheat kernels with bacterial antagonists |
publisher |
MDPI AG |
series |
International Journal of Environmental Research and Public Health |
issn |
1660-4601 |
publishDate |
2014-01-01 |
description |
Fusarium graminearum is the main causal pathogen affecting small-grain cereals, and it produces deoxynivalenol, a kind of mycotoxin, which displays a wide range of toxic effects in human and animals. Bacterial strains isolated from peanut shells were investigated for their activities against F. graminearum by dual-culture plate and tip-culture assays. Among them, twenty strains exhibited potent inhibition to the growth of F. graminearum, and the inhibition rates ranged from 41.41% to 54.55% in dual-culture plate assay and 92.70% to 100% in tip-culture assay. Furthermore, eighteen strains reduced the production of deoxynivalenol by 16.69% to 90.30% in the wheat kernels assay. Finally, the strains with the strongest inhibitory activity were identified by morphological, physiological, biochemical methods and also 16S rDNA and gyrA gene analysis as Bacillus amyloliquefaciens. The current study highlights the potential application of antagonistic microorganisms and their metabolites in the prevention of fungal growth and mycotoxin production in wheat kernels. As a biological strategy, it might avoid safety problems and nutrition loss which always caused by physical and chemical strategies. |
topic |
bacterial antagonists biocontrol deoxynivalenol Fusarium graminearum wheat kernels |
url |
http://www.mdpi.com/1660-4601/11/1/1094 |
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