Potential biomarkers for the clinical prognosis of severe dengue
Currently, several assays can confirm acute dengue infection at the point-of-care. However, none of these assays can predict the severity of the disease symptoms. A prognosis test that predicts the likelihood of a dengue patient to develop a severe form of the disease could permit more efficient pat...
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Instituto Oswaldo Cruz, Ministério da Saúde
2013-09-01
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doaj-06c30ad0d3ed434285647683c0e7d8782020-11-24T23:10:30ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.1678-80602013-09-01108675576210.1590/0074-0276108062013012S0074-02762013000600755Potential biomarkers for the clinical prognosis of severe dengueMayara Marques Carneiro da SilvaLaura Helena Vega Gonzales GilErnesto Torres de Azevedo Marques JuniorCarlos Eduardo Calzavara-SilvaCurrently, several assays can confirm acute dengue infection at the point-of-care. However, none of these assays can predict the severity of the disease symptoms. A prognosis test that predicts the likelihood of a dengue patient to develop a severe form of the disease could permit more efficient patient triage and treatment. We hypothesise that mRNA expression of apoptosis and innate immune response-related genes will be differentially regulated during the early stages of dengue and might predict the clinical outcome. Aiming to identify biomarkers for dengue prognosis, we extracted mRNA from the peripheral blood mononuclear cells of mild and severe dengue patients during the febrile stage of the disease to measure the expression levels of selected genes by quantitative polymerase chain reaction. The selected candidate biomarkers were previously identified by our group as differentially expressed in microarray studies. We verified that the mRNA coding for CFD, MAGED1, PSMB9, PRDX4 and FCGR3B were differentially expressed between patients who developed clinical symptoms associated with the mild type of dengue and patients who showed clinical symptoms associated with severe dengue. We suggest that this gene expression panel could putatively serve as biomarkers for the clinical prognosis of dengue haemorrhagic fever.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000600755&lng=en&tlng=endenguebiomarkersapoptosisinnate immunityquantitative real-time PCR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mayara Marques Carneiro da Silva Laura Helena Vega Gonzales Gil Ernesto Torres de Azevedo Marques Junior Carlos Eduardo Calzavara-Silva |
spellingShingle |
Mayara Marques Carneiro da Silva Laura Helena Vega Gonzales Gil Ernesto Torres de Azevedo Marques Junior Carlos Eduardo Calzavara-Silva Potential biomarkers for the clinical prognosis of severe dengue Memórias do Instituto Oswaldo Cruz. dengue biomarkers apoptosis innate immunity quantitative real-time PCR |
author_facet |
Mayara Marques Carneiro da Silva Laura Helena Vega Gonzales Gil Ernesto Torres de Azevedo Marques Junior Carlos Eduardo Calzavara-Silva |
author_sort |
Mayara Marques Carneiro da Silva |
title |
Potential biomarkers for the clinical prognosis of severe dengue |
title_short |
Potential biomarkers for the clinical prognosis of severe dengue |
title_full |
Potential biomarkers for the clinical prognosis of severe dengue |
title_fullStr |
Potential biomarkers for the clinical prognosis of severe dengue |
title_full_unstemmed |
Potential biomarkers for the clinical prognosis of severe dengue |
title_sort |
potential biomarkers for the clinical prognosis of severe dengue |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
1678-8060 |
publishDate |
2013-09-01 |
description |
Currently, several assays can confirm acute dengue infection at the point-of-care. However, none of these assays can predict the severity of the disease symptoms. A prognosis test that predicts the likelihood of a dengue patient to develop a severe form of the disease could permit more efficient patient triage and treatment. We hypothesise that mRNA expression of apoptosis and innate immune response-related genes will be differentially regulated during the early stages of dengue and might predict the clinical outcome. Aiming to identify biomarkers for dengue prognosis, we extracted mRNA from the peripheral blood mononuclear cells of mild and severe dengue patients during the febrile stage of the disease to measure the expression levels of selected genes by quantitative polymerase chain reaction. The selected candidate biomarkers were previously identified by our group as differentially expressed in microarray studies. We verified that the mRNA coding for CFD, MAGED1, PSMB9, PRDX4 and FCGR3B were differentially expressed between patients who developed clinical symptoms associated with the mild type of dengue and patients who showed clinical symptoms associated with severe dengue. We suggest that this gene expression panel could putatively serve as biomarkers for the clinical prognosis of dengue haemorrhagic fever. |
topic |
dengue biomarkers apoptosis innate immunity quantitative real-time PCR |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000600755&lng=en&tlng=en |
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