A One Health Comparative Assessment of Antimicrobial Resistance in Generic and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> from Beef Production, Sewage and Clinical Settings
This study aimed to compare antimicrobial resistance (AMR) in extended-spectrum cephalosporin-resistant and generic <i>Escherichia coli</i> from a One Health continuum of the beef production system in Alberta, Canada. A total of 705 extended-spectrum cephalosporin-resistant <i>E. c...
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MDPI AG
2020-06-01
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Online Access: | https://www.mdpi.com/2076-2607/8/6/885 |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Emelia H. Adator Claudia Narvaez-Bravo Rahat Zaheer Shaun R. Cook Lisa Tymensen Sherry J. Hannon Calvin W. Booker Deirdre Church Ron R. Read Tim A. McAllister |
spellingShingle |
Emelia H. Adator Claudia Narvaez-Bravo Rahat Zaheer Shaun R. Cook Lisa Tymensen Sherry J. Hannon Calvin W. Booker Deirdre Church Ron R. Read Tim A. McAllister A One Health Comparative Assessment of Antimicrobial Resistance in Generic and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> from Beef Production, Sewage and Clinical Settings Microorganisms antimicrobial resistance extended-spectrum beta-lactamase (ESBL) one health beef sewage |
author_facet |
Emelia H. Adator Claudia Narvaez-Bravo Rahat Zaheer Shaun R. Cook Lisa Tymensen Sherry J. Hannon Calvin W. Booker Deirdre Church Ron R. Read Tim A. McAllister |
author_sort |
Emelia H. Adator |
title |
A One Health Comparative Assessment of Antimicrobial Resistance in Generic and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> from Beef Production, Sewage and Clinical Settings |
title_short |
A One Health Comparative Assessment of Antimicrobial Resistance in Generic and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> from Beef Production, Sewage and Clinical Settings |
title_full |
A One Health Comparative Assessment of Antimicrobial Resistance in Generic and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> from Beef Production, Sewage and Clinical Settings |
title_fullStr |
A One Health Comparative Assessment of Antimicrobial Resistance in Generic and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> from Beef Production, Sewage and Clinical Settings |
title_full_unstemmed |
A One Health Comparative Assessment of Antimicrobial Resistance in Generic and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> from Beef Production, Sewage and Clinical Settings |
title_sort |
one health comparative assessment of antimicrobial resistance in generic and extended-spectrum cephalosporin-resistant <i>escherichia coli</i> from beef production, sewage and clinical settings |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2020-06-01 |
description |
This study aimed to compare antimicrobial resistance (AMR) in extended-spectrum cephalosporin-resistant and generic <i>Escherichia coli</i> from a One Health continuum of the beef production system in Alberta, Canada. A total of 705 extended-spectrum cephalosporin-resistant <i>E. coli</i> (ESC<sup>r</sup>) were obtained from: cattle feces (CFeces, <i>n</i> = 382), catch basins (CBasins, <i>n</i> = 137), surrounding streams (SStreams, <i>n</i> = 59), beef processing plants (BProcessing, <i>n</i> = 4), municipal sewage (MSewage; <i>n</i> = 98) and human clinical specimens (CHumans, <i>n</i> = 25). Generic isolates (663) included: CFeces (<i>n</i> = 142), CBasins (<i>n</i> = 185), SStreams (<i>n</i> = 81), BProcessing (<i>n</i> = 159) and MSewage (<i>n</i> = 96). All isolates were screened for antimicrobial susceptibility to 9 antimicrobials and two clavulanic acid combinations. In ESC<sup>r</sup><i>,</i> oxytetracycline (87.7%), ampicillin (84.4%) and streptomycin (73.8%) resistance phenotypes were the most common, with source influencing AMR prevalence (<i>p</i> < 0.001). In generic <i>E. coli,</i> oxytetracycline (51.1%), streptomycin (22.6%), ampicillin (22.5%) and sulfisoxazole (14.3%) resistance were most common. Overall, 88.8% of ESC<sup>r</sup>, and 26.7% of generic isolates exhibited multi-drug resistance (MDR). MDR in ESC<sup>r</sup> was high from all sources: CFeces (97.1%), MSewage (96.9%), CHumans (96%), BProcessing (100%), CBasins (70.5%) and SStreams (61.4%). MDR in generic <i>E. coli</i> was lower with CFeces (45.1%), CBasins (34.6%), SStreams (23.5%), MSewage (13.6%) and BProcessing (10.7%). ESBL phenotypes were confirmed in 24.7% (<i>n</i> = 174) ESC<sup>r</sup> and 0.6% of generic <i>E. coli</i>. Prevalence of <i>bla</i> genes in ESC<sup>r</sup> were <i>bla</i><sub>CTXM</sub> (30.1%), <i>bla</i><sub>CTXM-1</sub> (21.6%), <i>bla</i><sub>TEM</sub> (20%), <i>bla</i><sub>CTXM-9</sub> (7.9%), <i>bla</i><sub>OXA</sub> (3.0%), <i>bla</i><sub>CTXM-2</sub> (6.4%), <i>bla</i><sub>SHV</sub> (1.4%) and AmpC β-lactamase <i>bla</i><sub>CMY</sub> (81.3%). The lower AMR in ESC<sup>r</sup> from SStreams and BProcessing and higher AMR in CHumans and CFeces likely reflects antimicrobial use in these environments. Although MDR levels were higher in ESC<sup>r</sup> as compared to generic <i>E. coli</i>, AMR to the same antimicrobials ranked high in both ESC<sup>r</sup> and generic <i>E. coli</i> sub-populations. This suggests that both sub-populations reflect similar AMR trends and are equally useful for AMR surveillance. Considering that MDR ESC<sup>r</sup> MSewage isolates were obtained without enrichment, while those from CFeces were obtained with enrichment, MSewage may serve as a hot spot for MDR emergence and dissemination. |
topic |
antimicrobial resistance extended-spectrum beta-lactamase (ESBL) one health beef sewage |
url |
https://www.mdpi.com/2076-2607/8/6/885 |
work_keys_str_mv |
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doaj-06c33d81f12241db9e0f36769522cc5f2020-11-25T03:09:59ZengMDPI AGMicroorganisms2076-26072020-06-01888588510.3390/microorganisms8060885A One Health Comparative Assessment of Antimicrobial Resistance in Generic and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> from Beef Production, Sewage and Clinical SettingsEmelia H. Adator0Claudia Narvaez-Bravo1Rahat Zaheer2Shaun R. Cook3Lisa Tymensen4Sherry J. Hannon5Calvin W. Booker6Deirdre Church7Ron R. Read8Tim A. McAllister9Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaLethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB T1J 4B1, CanadaIrrigation and Farm Water Branch, Alberta Agriculture and Forestry, Lethbridge, AB T1J 4V6, CanadaIrrigation and Farm Water Branch, Alberta Agriculture and Forestry, Lethbridge, AB T1J 4V6, CanadaHealth Management Services Ltd, Okotoks, AB T1S 2A2, CanadaHealth Management Services Ltd, Okotoks, AB T1S 2A2, CanadaDepartment of Pathology & Laboratory Medicine and Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1, CanadaDepartment of Pathology & Laboratory Medicine and Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1, CanadaDepartment of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaThis study aimed to compare antimicrobial resistance (AMR) in extended-spectrum cephalosporin-resistant and generic <i>Escherichia coli</i> from a One Health continuum of the beef production system in Alberta, Canada. A total of 705 extended-spectrum cephalosporin-resistant <i>E. coli</i> (ESC<sup>r</sup>) were obtained from: cattle feces (CFeces, <i>n</i> = 382), catch basins (CBasins, <i>n</i> = 137), surrounding streams (SStreams, <i>n</i> = 59), beef processing plants (BProcessing, <i>n</i> = 4), municipal sewage (MSewage; <i>n</i> = 98) and human clinical specimens (CHumans, <i>n</i> = 25). Generic isolates (663) included: CFeces (<i>n</i> = 142), CBasins (<i>n</i> = 185), SStreams (<i>n</i> = 81), BProcessing (<i>n</i> = 159) and MSewage (<i>n</i> = 96). All isolates were screened for antimicrobial susceptibility to 9 antimicrobials and two clavulanic acid combinations. In ESC<sup>r</sup><i>,</i> oxytetracycline (87.7%), ampicillin (84.4%) and streptomycin (73.8%) resistance phenotypes were the most common, with source influencing AMR prevalence (<i>p</i> < 0.001). In generic <i>E. coli,</i> oxytetracycline (51.1%), streptomycin (22.6%), ampicillin (22.5%) and sulfisoxazole (14.3%) resistance were most common. Overall, 88.8% of ESC<sup>r</sup>, and 26.7% of generic isolates exhibited multi-drug resistance (MDR). MDR in ESC<sup>r</sup> was high from all sources: CFeces (97.1%), MSewage (96.9%), CHumans (96%), BProcessing (100%), CBasins (70.5%) and SStreams (61.4%). MDR in generic <i>E. coli</i> was lower with CFeces (45.1%), CBasins (34.6%), SStreams (23.5%), MSewage (13.6%) and BProcessing (10.7%). ESBL phenotypes were confirmed in 24.7% (<i>n</i> = 174) ESC<sup>r</sup> and 0.6% of generic <i>E. coli</i>. Prevalence of <i>bla</i> genes in ESC<sup>r</sup> were <i>bla</i><sub>CTXM</sub> (30.1%), <i>bla</i><sub>CTXM-1</sub> (21.6%), <i>bla</i><sub>TEM</sub> (20%), <i>bla</i><sub>CTXM-9</sub> (7.9%), <i>bla</i><sub>OXA</sub> (3.0%), <i>bla</i><sub>CTXM-2</sub> (6.4%), <i>bla</i><sub>SHV</sub> (1.4%) and AmpC β-lactamase <i>bla</i><sub>CMY</sub> (81.3%). The lower AMR in ESC<sup>r</sup> from SStreams and BProcessing and higher AMR in CHumans and CFeces likely reflects antimicrobial use in these environments. Although MDR levels were higher in ESC<sup>r</sup> as compared to generic <i>E. coli</i>, AMR to the same antimicrobials ranked high in both ESC<sup>r</sup> and generic <i>E. coli</i> sub-populations. This suggests that both sub-populations reflect similar AMR trends and are equally useful for AMR surveillance. Considering that MDR ESC<sup>r</sup> MSewage isolates were obtained without enrichment, while those from CFeces were obtained with enrichment, MSewage may serve as a hot spot for MDR emergence and dissemination.https://www.mdpi.com/2076-2607/8/6/885antimicrobial resistanceextended-spectrum beta-lactamase (ESBL)one healthbeefsewage |