Genome-Wide Identification, Characterization, and Expression Analysis of Small RNA Biogenesis Purveyors Reveal Their Role in Regulation of Biotic Stress Responses in Three Legume Crops
Biotic stress in legume crops is one of the major threats to crop yield and productivity. Being sessile organisms, plants have evolved a myriad of mechanisms to combat different stresses imposed on them. One such mechanism, deciphered in the last decade, is small RNA (sRNA) mediated defense in plant...
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doaj-06ca6c154a07411da712c4c38c42f2892020-11-24T23:49:58ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-04-01810.3389/fpls.2017.00488238864Genome-Wide Identification, Characterization, and Expression Analysis of Small RNA Biogenesis Purveyors Reveal Their Role in Regulation of Biotic Stress Responses in Three Legume CropsRajeev K. Varshney0Rajeev K. Varshney1Vanika Garg2Vanika Garg3Gaurav Agarwal4Lekha T. Pazhamala5Spurthi N. Nayak6Himabindu Kudapa7Aamir W. Khan8Dadakhalandar Doddamani9Mamta Sharma10P. B. Kavi Kishor11Center of Excellence in Genomics, International Crops Research Institute for the Semi-Arid TropicsHyderabad, IndiaSchool of Agriculture and Environment, The University of Western AustraliaCrawley, WA, AustraliaCenter of Excellence in Genomics, International Crops Research Institute for the Semi-Arid TropicsHyderabad, IndiaDepartment of Genetics, Osmania UniversityHyderabad, IndiaCenter of Excellence in Genomics, International Crops Research Institute for the Semi-Arid TropicsHyderabad, IndiaCenter of Excellence in Genomics, International Crops Research Institute for the Semi-Arid TropicsHyderabad, IndiaCenter of Excellence in Genomics, International Crops Research Institute for the Semi-Arid TropicsHyderabad, IndiaCenter of Excellence in Genomics, International Crops Research Institute for the Semi-Arid TropicsHyderabad, IndiaCenter of Excellence in Genomics, International Crops Research Institute for the Semi-Arid TropicsHyderabad, IndiaCenter of Excellence in Genomics, International Crops Research Institute for the Semi-Arid TropicsHyderabad, IndiaCenter of Excellence in Genomics, International Crops Research Institute for the Semi-Arid TropicsHyderabad, IndiaDepartment of Genetics, Osmania UniversityHyderabad, IndiaBiotic stress in legume crops is one of the major threats to crop yield and productivity. Being sessile organisms, plants have evolved a myriad of mechanisms to combat different stresses imposed on them. One such mechanism, deciphered in the last decade, is small RNA (sRNA) mediated defense in plants. Small RNAs (sRNAs) have emerged as one of the major players in gene expression regulation in plants during developmental stages and under stress conditions. They are known to act both at transcriptional and post-transcriptional levels. Dicer-like (DCL), Argonaute (AGO), and RNA dependent RNA polymerase (RDR) constitute the major components of sRNA biogenesis machinery and are known to play a significant role in combating biotic and abiotic stresses. This study is, therefore, focused on identification and characterization of sRNA biogenesis proteins in three important legume crops, namely chickpea, pigeonpea, and groundnut. Phylogenetic analysis of these proteins between legume species classified them into distinct clades and suggests the evolutionary conservation of these genes across the members of Papillionidoids subfamily. Variable expression of sRNA biogenesis genes in response to the biotic stresses among the three legumes indicate the possible existence of specialized regulatory mechanisms in different legumes. This is the first ever study to understand the role of sRNA biogenesis genes in response to pathogen attacks in the studied legumes.http://journal.frontiersin.org/article/10.3389/fpls.2017.00488/fullAGODCLRDRgene expressionbiotic stressPapillionidoids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rajeev K. Varshney Rajeev K. Varshney Vanika Garg Vanika Garg Gaurav Agarwal Lekha T. Pazhamala Spurthi N. Nayak Himabindu Kudapa Aamir W. Khan Dadakhalandar Doddamani Mamta Sharma P. B. Kavi Kishor |
spellingShingle |
Rajeev K. Varshney Rajeev K. Varshney Vanika Garg Vanika Garg Gaurav Agarwal Lekha T. Pazhamala Spurthi N. Nayak Himabindu Kudapa Aamir W. Khan Dadakhalandar Doddamani Mamta Sharma P. B. Kavi Kishor Genome-Wide Identification, Characterization, and Expression Analysis of Small RNA Biogenesis Purveyors Reveal Their Role in Regulation of Biotic Stress Responses in Three Legume Crops Frontiers in Plant Science AGO DCL RDR gene expression biotic stress Papillionidoids |
author_facet |
Rajeev K. Varshney Rajeev K. Varshney Vanika Garg Vanika Garg Gaurav Agarwal Lekha T. Pazhamala Spurthi N. Nayak Himabindu Kudapa Aamir W. Khan Dadakhalandar Doddamani Mamta Sharma P. B. Kavi Kishor |
author_sort |
Rajeev K. Varshney |
title |
Genome-Wide Identification, Characterization, and Expression Analysis of Small RNA Biogenesis Purveyors Reveal Their Role in Regulation of Biotic Stress Responses in Three Legume Crops |
title_short |
Genome-Wide Identification, Characterization, and Expression Analysis of Small RNA Biogenesis Purveyors Reveal Their Role in Regulation of Biotic Stress Responses in Three Legume Crops |
title_full |
Genome-Wide Identification, Characterization, and Expression Analysis of Small RNA Biogenesis Purveyors Reveal Their Role in Regulation of Biotic Stress Responses in Three Legume Crops |
title_fullStr |
Genome-Wide Identification, Characterization, and Expression Analysis of Small RNA Biogenesis Purveyors Reveal Their Role in Regulation of Biotic Stress Responses in Three Legume Crops |
title_full_unstemmed |
Genome-Wide Identification, Characterization, and Expression Analysis of Small RNA Biogenesis Purveyors Reveal Their Role in Regulation of Biotic Stress Responses in Three Legume Crops |
title_sort |
genome-wide identification, characterization, and expression analysis of small rna biogenesis purveyors reveal their role in regulation of biotic stress responses in three legume crops |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2017-04-01 |
description |
Biotic stress in legume crops is one of the major threats to crop yield and productivity. Being sessile organisms, plants have evolved a myriad of mechanisms to combat different stresses imposed on them. One such mechanism, deciphered in the last decade, is small RNA (sRNA) mediated defense in plants. Small RNAs (sRNAs) have emerged as one of the major players in gene expression regulation in plants during developmental stages and under stress conditions. They are known to act both at transcriptional and post-transcriptional levels. Dicer-like (DCL), Argonaute (AGO), and RNA dependent RNA polymerase (RDR) constitute the major components of sRNA biogenesis machinery and are known to play a significant role in combating biotic and abiotic stresses. This study is, therefore, focused on identification and characterization of sRNA biogenesis proteins in three important legume crops, namely chickpea, pigeonpea, and groundnut. Phylogenetic analysis of these proteins between legume species classified them into distinct clades and suggests the evolutionary conservation of these genes across the members of Papillionidoids subfamily. Variable expression of sRNA biogenesis genes in response to the biotic stresses among the three legumes indicate the possible existence of specialized regulatory mechanisms in different legumes. This is the first ever study to understand the role of sRNA biogenesis genes in response to pathogen attacks in the studied legumes. |
topic |
AGO DCL RDR gene expression biotic stress Papillionidoids |
url |
http://journal.frontiersin.org/article/10.3389/fpls.2017.00488/full |
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