Effect of Geometrical Structure, Drying, and Synthetic Method on Aminated Chitosan-Coated Magnetic Nanoparticles Utility for HSA Effective Immobilization

• Main Authors: Marta Ziegler-Borowska, Kinga Mylkie, Mariana Kozlowska, Pawel Nowak, Dorota Chelminiak-Dudkiewicz, Anna Kozakiewicz, Anna Ilnicka, Anna Kaczmarek-Kedziera
• Format: Article
• Language: English
• Published: MDPI AG 2019-05-01
• Series: Molecules
• Subjects: aminated chitosan; magnetic nanoparticles; HSA; protein immobilization; molecular dynamics simulations; solvent-free amination
• Online Access: https://www.mdpi.com/1420-3049/24/10/1925

Human serum albumin (HSA) is one of the most frequently immobilized proteins on the surface of carriers, including magnetic nanoparticles. This is because the drug−HSA interaction study is one of the basic pharmacokinetic parameters determined for drugs. In spite of many works describing the immobilization of HSA and the binding of active substances, research describing the influence of the used support on the effectiveness of immobilization is missing. There are also no reports about the effect of the support drying method on the effectiveness of protein immobilization. This paper examines the effect of both the method of functionalizing the polymer coating covering magnetic nanoparticles (MNPs), and the drying methods for the immobilization of HSA. Albumin was immobilized on three types of aminated chitosan-coated nanoparticles with a different content of amino groups long distanced from the surface Fe₃O₄-CS-Et(NH₂)_1−3. The obtained results showed that both the synthesis method and the method of drying nanoparticles have a large impact on the effectiveness of immobilization. Due to the fact that the results obtained for Fe₃O₄-CS-Et(NH₂)₂ significantly differ from those obtained for the others, the influence of the geometry of the shell structure on the ability to bind HSA was also explained by molecular dynamics.