Design of Biotin-Functionalized Luminescent Quantum Dots
We report the design and synthesis of a tetraethylene glycol- (TEG-) based bidentate ligand functionalized with dihydrolipoic acid (DHLA) and biotin (DHLA—TEG—biotin) to promote biocompatibility of luminescent quantum dots (QD's). This new ligand readily binds to CdSe—ZnS core-shell QDs via su...
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2007-01-01
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doaj-06e4ca4034244d218ad58f4e38267fc92020-11-25T01:13:59ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512007-01-01200710.1155/2007/9065190651Design of Biotin-Functionalized Luminescent Quantum DotsKimihiro Susumu0H. Tetsuo Uyeda1Igor L. Medintz2Hedi Mattoussi3Division of Optical Sciences, U.S. Naval Research Laboratory, Washington, DC 20375, USADivision of Optical Sciences, U.S. Naval Research Laboratory, Washington, DC 20375, USACenter for Bio/Molecular Science and Engineering, U.S. Naval Research Laboratory, Washington, DC 20375, USADivision of Optical Sciences, U.S. Naval Research Laboratory, Washington, DC 20375, USAWe report the design and synthesis of a tetraethylene glycol- (TEG-) based bidentate ligand functionalized with dihydrolipoic acid (DHLA) and biotin (DHLA—TEG—biotin) to promote biocompatibility of luminescent quantum dots (QD's). This new ligand readily binds to CdSe—ZnS core-shell QDs via surface ligand exchange. QDs capped with a mixture of DHLA and DHLA—TEG—biotin or polyethylene glycol- (PEG-) (molecular weight average ∼600) modified DHLA (DHLA—PEG600) and DHLA—TEG—biotin are easily dispersed in aqueous buffer solutions. In particular, homogeneous buffer solutions of QDs capped with a mixture of DHLA—PEG600 and DHLA—TEG—biotin that are stable over broad pH range have been prepared. QDs coated with mixtures of DHLA/DHLA—TEG—biotin and with DHLA—PEG600/DHLA—TEG—biotin were tested in surface binding assays and the results indicate that biotin groups on the QD surface interact specifically with NeutrAvidin-functionalized microtiter well plates.http://dx.doi.org/10.1155/2007/90651 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kimihiro Susumu H. Tetsuo Uyeda Igor L. Medintz Hedi Mattoussi |
spellingShingle |
Kimihiro Susumu H. Tetsuo Uyeda Igor L. Medintz Hedi Mattoussi Design of Biotin-Functionalized Luminescent Quantum Dots Journal of Biomedicine and Biotechnology |
author_facet |
Kimihiro Susumu H. Tetsuo Uyeda Igor L. Medintz Hedi Mattoussi |
author_sort |
Kimihiro Susumu |
title |
Design of Biotin-Functionalized Luminescent Quantum Dots |
title_short |
Design of Biotin-Functionalized Luminescent Quantum Dots |
title_full |
Design of Biotin-Functionalized Luminescent Quantum Dots |
title_fullStr |
Design of Biotin-Functionalized Luminescent Quantum Dots |
title_full_unstemmed |
Design of Biotin-Functionalized Luminescent Quantum Dots |
title_sort |
design of biotin-functionalized luminescent quantum dots |
publisher |
Hindawi Limited |
series |
Journal of Biomedicine and Biotechnology |
issn |
1110-7243 1110-7251 |
publishDate |
2007-01-01 |
description |
We report the design and synthesis of a tetraethylene glycol- (TEG-) based bidentate ligand functionalized with dihydrolipoic acid (DHLA) and biotin (DHLA—TEG—biotin) to promote biocompatibility of luminescent quantum dots (QD's). This new ligand readily binds to CdSe—ZnS core-shell QDs via surface ligand exchange. QDs capped with a mixture of DHLA and DHLA—TEG—biotin or polyethylene glycol- (PEG-) (molecular weight average ∼600) modified DHLA (DHLA—PEG600) and DHLA—TEG—biotin are easily dispersed in aqueous buffer solutions. In particular, homogeneous buffer solutions of QDs capped with a mixture of DHLA—PEG600 and DHLA—TEG—biotin that are stable over broad pH range have been prepared. QDs coated with mixtures of DHLA/DHLA—TEG—biotin and with DHLA—PEG600/DHLA—TEG—biotin were tested in surface binding assays and the results indicate that biotin groups on the QD surface interact specifically with NeutrAvidin-functionalized microtiter well plates. |
url |
http://dx.doi.org/10.1155/2007/90651 |
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