A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales

Monomeric near-infrared (NIR) fluorescent proteins (FPs) from bacterial phytochromes bring potential advantages, but their brightness in cells is lower than dimeric NIR FPs. Here the authors develop enhanced monomeric NIR FPs enabling imaging across different scales without the trade-off between bri...

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Main Authors:	Mikhail E. Matlashov, Daria M. Shcherbakova, Jonatan Alvelid, Mikhail Baloban, Francesca Pennacchietti, Anton A. Shemetov, Ilaria Testa, Vladislav V. Verkhusha
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-01-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-13897-6

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spelling	doaj-06f6d420b5f14b9d96bca6c15df9a3a72021-05-11T07:59:22ZengNature Publishing GroupNature Communications2041-17232020-01-0111111210.1038/s41467-019-13897-6A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scalesMikhail E. Matlashov0Daria M. Shcherbakova1Jonatan Alvelid2Mikhail Baloban3Francesca Pennacchietti4Anton A. Shemetov5Ilaria Testa6Vladislav V. Verkhusha7Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, BronxDepartment of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, BronxDepartment of Applied Physics and Science for Life Laboratory, KTH Royal Institute of TechnologyDepartment of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, BronxDepartment of Applied Physics and Science for Life Laboratory, KTH Royal Institute of TechnologyDepartment of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, BronxDepartment of Applied Physics and Science for Life Laboratory, KTH Royal Institute of TechnologyDepartment of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, BronxMonomeric near-infrared (NIR) fluorescent proteins (FPs) from bacterial phytochromes bring potential advantages, but their brightness in cells is lower than dimeric NIR FPs. Here the authors develop enhanced monomeric NIR FPs enabling imaging across different scales without the trade-off between brightness and monomeric state.https://doi.org/10.1038/s41467-019-13897-6
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Mikhail E. Matlashov Daria M. Shcherbakova Jonatan Alvelid Mikhail Baloban Francesca Pennacchietti Anton A. Shemetov Ilaria Testa Vladislav V. Verkhusha
spellingShingle	Mikhail E. Matlashov Daria M. Shcherbakova Jonatan Alvelid Mikhail Baloban Francesca Pennacchietti Anton A. Shemetov Ilaria Testa Vladislav V. Verkhusha A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales Nature Communications
author_facet	Mikhail E. Matlashov Daria M. Shcherbakova Jonatan Alvelid Mikhail Baloban Francesca Pennacchietti Anton A. Shemetov Ilaria Testa Vladislav V. Verkhusha
author_sort	Mikhail E. Matlashov
title	A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
title_short	A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
title_full	A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
title_fullStr	A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
title_full_unstemmed	A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
title_sort	set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-01-01
description	Monomeric near-infrared (NIR) fluorescent proteins (FPs) from bacterial phytochromes bring potential advantages, but their brightness in cells is lower than dimeric NIR FPs. Here the authors develop enhanced monomeric NIR FPs enabling imaging across different scales without the trade-off between brightness and monomeric state.
url	https://doi.org/10.1038/s41467-019-13897-6
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A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales

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