Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size
The polymerization/depolymerization dynamics of microtubules (MTs) have been reported to contribute to control of the size and shape of spindles, but quantitative analysis of how the size and shape correlate with the amount and density of MTs in the spindle remains incomplete. Here, we measured the...
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doaj-070d864c6c6f48068899dcb4f88fb6152020-11-25T01:30:15ZengElsevierCell Reports2211-12472013-10-0151445010.1016/j.celrep.2013.09.021Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle SizeJun Takagi0Takeshi Itabashi1Kazuya Suzuki2Tarun M. Kapoor3Yuta Shimamoto4Shin’ichi Ishiwata5Department of Physics, Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, JapanDepartment of Physics, Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, JapanDepartment of Physics, Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, JapanLaboratory of Chemistry and Cell Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USALaboratory of Chemistry and Cell Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USADepartment of Physics, Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan The polymerization/depolymerization dynamics of microtubules (MTs) have been reported to contribute to control of the size and shape of spindles, but quantitative analysis of how the size and shape correlate with the amount and density of MTs in the spindle remains incomplete. Here, we measured these parameters using 3D microscopy of meiotic spindles that self-organized in Xenopus egg extracts and presented a simple equation describing the relationship among these parameters. To examine the validity of the equation, we cut the spindle into two fragments along the pole-to-pole axis by micromanipulation techniques that rapidly decrease the amount of MTs. The spheroidal shape spontaneously recovered within 5 min, but the size of each fragment remained small. The equation we obtained quantitatively describes how the spindle size correlates with the amount of MTs while maintaining the shape and the MT density. http://www.sciencedirect.com/science/article/pii/S2211124713005445 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jun Takagi Takeshi Itabashi Kazuya Suzuki Tarun M. Kapoor Yuta Shimamoto Shin’ichi Ishiwata |
spellingShingle |
Jun Takagi Takeshi Itabashi Kazuya Suzuki Tarun M. Kapoor Yuta Shimamoto Shin’ichi Ishiwata Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size Cell Reports |
author_facet |
Jun Takagi Takeshi Itabashi Kazuya Suzuki Tarun M. Kapoor Yuta Shimamoto Shin’ichi Ishiwata |
author_sort |
Jun Takagi |
title |
Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size |
title_short |
Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size |
title_full |
Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size |
title_fullStr |
Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size |
title_full_unstemmed |
Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size |
title_sort |
using micromanipulation to analyze control of vertebrate meiotic spindle size |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2013-10-01 |
description |
The polymerization/depolymerization dynamics of microtubules (MTs) have been reported to contribute to control of the size and shape of spindles, but quantitative analysis of how the size and shape correlate with the amount and density of MTs in the spindle remains incomplete. Here, we measured these parameters using 3D microscopy of meiotic spindles that self-organized in Xenopus egg extracts and presented a simple equation describing the relationship among these parameters. To examine the validity of the equation, we cut the spindle into two fragments along the pole-to-pole axis by micromanipulation techniques that rapidly decrease the amount of MTs. The spheroidal shape spontaneously recovered within 5 min, but the size of each fragment remained small. The equation we obtained quantitatively describes how the spindle size correlates with the amount of MTs while maintaining the shape and the MT density.
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url |
http://www.sciencedirect.com/science/article/pii/S2211124713005445 |
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