Effection of cordycepin on Inhibition proliferation by activating autophagy in HepG2 cell

Cordycepin, the main active component of Cordyceps militaris, possesses a variety of biological activities. This paper investigated the inhibition of cordycepin on liver tumors. HepG2 cells were treated with cordycepin. The inhibition rates of cell proliferation was detected by MTT, LC3 protein was...

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Main Authors: Tianjiao Li, Bijun Cheng, Fenglin Li
Format: Article
Language:English
Published: EDP Sciences 2020-01-01
Series:E3S Web of Conferences
Online Access:https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/49/e3sconf_astfe2020_02026.pdf
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spelling doaj-071852b44196428392641048fc2bd7ac2021-04-02T11:12:46ZengEDP SciencesE3S Web of Conferences2267-12422020-01-011890202610.1051/e3sconf/202018902026e3sconf_astfe2020_02026Effection of cordycepin on Inhibition proliferation by activating autophagy in HepG2 cellTianjiao Li0Bijun Cheng1Fenglin Li2College of Food Engineering, Jilin Agricultural Science and Technology UniversityCollege of Food Engineering, Jilin Agricultural Science and Technology UniversityCollege of Food Engineering, Jilin Agricultural Science and Technology UniversityCordycepin, the main active component of Cordyceps militaris, possesses a variety of biological activities. This paper investigated the inhibition of cordycepin on liver tumors. HepG2 cells were treated with cordycepin. The inhibition rates of cell proliferation was detected by MTT, LC3 protein was detected by immunofluorescence method, autophagy was observed by transmission electron microscopy, autophagy related protein was detected by Western blot. The results showed that cordycepin significantly inhibited the proliferation of HepG2 cells, and significantly increased the expression level of LC3 protein (<0.05). and the autophagy increased in the cells. The Atg-related protein analysis showed that cordycepin significantly reduced the p-mTOR / β-actin ratio and p62 / β-actin ratio (<0.01), and significantly increased the LC3II/LC3I ratio (P<0.01). The results suggested that cordycepin inhibited the proliferation by activating autophagy in HepG2 cells.https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/49/e3sconf_astfe2020_02026.pdf
collection DOAJ
language English
format Article
sources DOAJ
author Tianjiao Li
Bijun Cheng
Fenglin Li
spellingShingle Tianjiao Li
Bijun Cheng
Fenglin Li
Effection of cordycepin on Inhibition proliferation by activating autophagy in HepG2 cell
E3S Web of Conferences
author_facet Tianjiao Li
Bijun Cheng
Fenglin Li
author_sort Tianjiao Li
title Effection of cordycepin on Inhibition proliferation by activating autophagy in HepG2 cell
title_short Effection of cordycepin on Inhibition proliferation by activating autophagy in HepG2 cell
title_full Effection of cordycepin on Inhibition proliferation by activating autophagy in HepG2 cell
title_fullStr Effection of cordycepin on Inhibition proliferation by activating autophagy in HepG2 cell
title_full_unstemmed Effection of cordycepin on Inhibition proliferation by activating autophagy in HepG2 cell
title_sort effection of cordycepin on inhibition proliferation by activating autophagy in hepg2 cell
publisher EDP Sciences
series E3S Web of Conferences
issn 2267-1242
publishDate 2020-01-01
description Cordycepin, the main active component of Cordyceps militaris, possesses a variety of biological activities. This paper investigated the inhibition of cordycepin on liver tumors. HepG2 cells were treated with cordycepin. The inhibition rates of cell proliferation was detected by MTT, LC3 protein was detected by immunofluorescence method, autophagy was observed by transmission electron microscopy, autophagy related protein was detected by Western blot. The results showed that cordycepin significantly inhibited the proliferation of HepG2 cells, and significantly increased the expression level of LC3 protein (<0.05). and the autophagy increased in the cells. The Atg-related protein analysis showed that cordycepin significantly reduced the p-mTOR / β-actin ratio and p62 / β-actin ratio (<0.01), and significantly increased the LC3II/LC3I ratio (P<0.01). The results suggested that cordycepin inhibited the proliferation by activating autophagy in HepG2 cells.
url https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/49/e3sconf_astfe2020_02026.pdf
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AT bijuncheng effectionofcordycepinoninhibitionproliferationbyactivatingautophagyinhepg2cell
AT fenglinli effectionofcordycepinoninhibitionproliferationbyactivatingautophagyinhepg2cell
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