Bioavailability and the mechanism of action of a grape extract rich in polyphenols in cholesterol homeostasis
The bioaccessibility of a grape extract rich in polyphenols (GE) was determined in order to shed light on the mechanism of action in cholesterol homeostasis. GE's bioaccessibility and the antioxidant capacity of the bioaccessible fraction (BF) of GE were determined using a dynamic gastrointesti...
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doaj-072f04650bbe4618afca744a1fcb164e2021-04-29T04:45:53ZengElsevierJournal of Functional Foods1756-46462016-03-0121178185Bioavailability and the mechanism of action of a grape extract rich in polyphenols in cholesterol homeostasisMarisa Sanz-Buenhombre0Sonia Villanueva1Carlos Moro2Lidia Tomás-Cobos3Blanca Viadel4Alberto Guadarrama5R&D Department, Abrobiotec S.L., Ctra. San Bernardo s/n. Valbuena de Duero, 47359, Valladolid, Spain; Corresponding author. Ctra. San Bernardo s/n. Valbuena de Duero, 47359, Valladolid, Spain. Tel.: +34 983 68 33 15; fax: +34 902 430 189.R&D Department, Bodega Matarromera S.L., Ctra. San Bernardo s/n. Valbuena de Duero, 47359, Valladolid, SpainR&D Department, Bodega Matarromera S.L., Ctra. San Bernardo s/n. Valbuena de Duero, 47359, Valladolid, SpainR&D Department, AINIA Technology Center, C/ Benjamin Franklin 5-11. Paterna, 46980, Valencia, SpainR&D Department, AINIA Technology Center, C/ Benjamin Franklin 5-11. Paterna, 46980, Valencia, SpainR&D Department, Bodega Matarromera S.L., Ctra. San Bernardo s/n. Valbuena de Duero, 47359, Valladolid, SpainThe bioaccessibility of a grape extract rich in polyphenols (GE) was determined in order to shed light on the mechanism of action in cholesterol homeostasis. GE's bioaccessibility and the antioxidant capacity of the bioaccessible fraction (BF) of GE were determined using a dynamic gastrointestinal digester (DGD). BF and GE were used to elucidate GE's mechanism of action, using two cell lines (HepG2 and Caco2) and different biomarkers. It was demonstrated that gastrointestinal digestion increases GE's bioaccessibility, with the antioxidant capacity of BF being statistically superior to that of GE (p < 0.05). With GE, a statistically significant increase in the expression of the low density lipoprotein receptor (LDLr) was noted, as well as for the intestinal cholesterol transporter (NPC1.L1) (p < 0.05). Besides, a statistically significant reduction in the expression of the cholesterol 7 alpha-hydroxylase enzyme (CYP7A1) (p < 0.05) was noted in BF.http://www.sciencedirect.com/science/article/pii/S1756464615005915BioavailabilityCholesterolGrape extractHomeostasisMechanism of actionPolyphenols |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marisa Sanz-Buenhombre Sonia Villanueva Carlos Moro Lidia Tomás-Cobos Blanca Viadel Alberto Guadarrama |
spellingShingle |
Marisa Sanz-Buenhombre Sonia Villanueva Carlos Moro Lidia Tomás-Cobos Blanca Viadel Alberto Guadarrama Bioavailability and the mechanism of action of a grape extract rich in polyphenols in cholesterol homeostasis Journal of Functional Foods Bioavailability Cholesterol Grape extract Homeostasis Mechanism of action Polyphenols |
author_facet |
Marisa Sanz-Buenhombre Sonia Villanueva Carlos Moro Lidia Tomás-Cobos Blanca Viadel Alberto Guadarrama |
author_sort |
Marisa Sanz-Buenhombre |
title |
Bioavailability and the mechanism of action of a grape extract rich in polyphenols in cholesterol homeostasis |
title_short |
Bioavailability and the mechanism of action of a grape extract rich in polyphenols in cholesterol homeostasis |
title_full |
Bioavailability and the mechanism of action of a grape extract rich in polyphenols in cholesterol homeostasis |
title_fullStr |
Bioavailability and the mechanism of action of a grape extract rich in polyphenols in cholesterol homeostasis |
title_full_unstemmed |
Bioavailability and the mechanism of action of a grape extract rich in polyphenols in cholesterol homeostasis |
title_sort |
bioavailability and the mechanism of action of a grape extract rich in polyphenols in cholesterol homeostasis |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2016-03-01 |
description |
The bioaccessibility of a grape extract rich in polyphenols (GE) was determined in order to shed light on the mechanism of action in cholesterol homeostasis. GE's bioaccessibility and the antioxidant capacity of the bioaccessible fraction (BF) of GE were determined using a dynamic gastrointestinal digester (DGD). BF and GE were used to elucidate GE's mechanism of action, using two cell lines (HepG2 and Caco2) and different biomarkers. It was demonstrated that gastrointestinal digestion increases GE's bioaccessibility, with the antioxidant capacity of BF being statistically superior to that of GE (p < 0.05). With GE, a statistically significant increase in the expression of the low density lipoprotein receptor (LDLr) was noted, as well as for the intestinal cholesterol transporter (NPC1.L1) (p < 0.05). Besides, a statistically significant reduction in the expression of the cholesterol 7 alpha-hydroxylase enzyme (CYP7A1) (p < 0.05) was noted in BF. |
topic |
Bioavailability Cholesterol Grape extract Homeostasis Mechanism of action Polyphenols |
url |
http://www.sciencedirect.com/science/article/pii/S1756464615005915 |
work_keys_str_mv |
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