Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophy

Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophy

Imaging with time-of-flight secondary ion mass spectrometry (TOF-SIMS) has expanded very rapidly with the development of gold cluster ion sources (Au3+). It is now possible to acquire ion density maps (ion images) on a tissue section without any treatment and with a lateral resolution of few microme...

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Main Authors: David Touboul, Alain Brunelle, Frédéric Halgand, Sabine De La Porte, Olivier Laprévote
Format: Article
Language:English
Published: Elsevier 2005-07-01
Series:Journal of Lipid Research
Subjects:
colocalization
oxidative stress
degeneration
destructured cells
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520329898
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spelling doaj-072f5dc00e384013923d44e921ee7ac62021-04-27T04:43:57ZengElsevierJournal of Lipid Research0022-22752005-07-0146713881395Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophyDavid Touboul0Alain Brunelle1Frédéric Halgand2Sabine De La Porte3Olivier Laprévote4Laboratoire de Spectrométrie de Masse, Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2301, F91198 Gif sur Yvette Cedex, FranceTo whom correspondence should be addressed.; Laboratoire de Spectrométrie de Masse, Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2301, F91198 Gif sur Yvette Cedex, FranceLaboratoire de Spectrométrie de Masse, Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2301, F91198 Gif sur Yvette Cedex, FranceLaboratoire de Neurobiologie Cellulaire et Moléculaire, Institut de Neurobiologie Alfred Fessard, Centre National de la Recherche Scientifique, Unité Propre de Recherche 9040, F91198 Gif sur Yvette Cedex, FranceLaboratoire de Spectrométrie de Masse, Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2301, F91198 Gif sur Yvette Cedex, FranceImaging with time-of-flight secondary ion mass spectrometry (TOF-SIMS) has expanded very rapidly with the development of gold cluster ion sources (Au3+). It is now possible to acquire ion density maps (ion images) on a tissue section without any treatment and with a lateral resolution of few micrometers. In this article, we have taken advantage of this technique to study the degeneration/regeneration process in muscles of a Duchenne muscular dystrophy model mouse. Specific distribution of different lipid classes (fatty acids, triglycerides, phospholipids, tocopherol, coenzyme Q9, and cholesterol) allows us to distinguish three different regions on a mouse leg section: one is destroyed, another is degenerating (oxidative stress and deregulation of the phosphoinositol cycle), and the last one is stable.TOF-SIMS imaging shows the ability to localize directly on a tissue section a great number of lipid compounds that reflect the state of the cellular metabolism.http://www.sciencedirect.com/science/article/pii/S0022227520329898colocalizationoxidative stressdegenerationdestructured cells
collection DOAJ
language English
format Article
sources DOAJ
author David Touboul
Alain Brunelle
Frédéric Halgand
Sabine De La Porte
Olivier Laprévote
spellingShingle David Touboul
Alain Brunelle
Frédéric Halgand
Sabine De La Porte
Olivier Laprévote
Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophy
Journal of Lipid Research
colocalization
oxidative stress
degeneration
destructured cells
author_facet David Touboul
Alain Brunelle
Frédéric Halgand
Sabine De La Porte
Olivier Laprévote
author_sort David Touboul
title Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophy
title_short Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophy
title_full Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophy
title_fullStr Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophy
title_full_unstemmed Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophy
title_sort lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to duchenne muscular dystrophy
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 2005-07-01
description Imaging with time-of-flight secondary ion mass spectrometry (TOF-SIMS) has expanded very rapidly with the development of gold cluster ion sources (Au3+). It is now possible to acquire ion density maps (ion images) on a tissue section without any treatment and with a lateral resolution of few micrometers. In this article, we have taken advantage of this technique to study the degeneration/regeneration process in muscles of a Duchenne muscular dystrophy model mouse. Specific distribution of different lipid classes (fatty acids, triglycerides, phospholipids, tocopherol, coenzyme Q9, and cholesterol) allows us to distinguish three different regions on a mouse leg section: one is destroyed, another is degenerating (oxidative stress and deregulation of the phosphoinositol cycle), and the last one is stable.TOF-SIMS imaging shows the ability to localize directly on a tissue section a great number of lipid compounds that reflect the state of the cellular metabolism.
topic colocalization
oxidative stress
degeneration
destructured cells
url http://www.sciencedirect.com/science/article/pii/S0022227520329898
work_keys_str_mv AT davidtouboul lipidimagingbygoldclustertimeofflightsecondaryionmassspectrometryapplicationtoduchennemusculardystrophy
AT alainbrunelle lipidimagingbygoldclustertimeofflightsecondaryionmassspectrometryapplicationtoduchennemusculardystrophy
AT frederichalgand lipidimagingbygoldclustertimeofflightsecondaryionmassspectrometryapplicationtoduchennemusculardystrophy
AT sabinedelaporte lipidimagingbygoldclustertimeofflightsecondaryionmassspectrometryapplicationtoduchennemusculardystrophy
AT olivierlaprevote lipidimagingbygoldclustertimeofflightsecondaryionmassspectrometryapplicationtoduchennemusculardystrophy
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