The toxicity mechanism of toxic compounds from Euphorbiae pekinensis Radix on zebrafish embryos
Euphorbiae pekinensis Radix (EP) is effective in treating various diseases, but it’s toxicity is a major obstacle in use in clinical. Although EP was processed with vinegar to reduce it’s toxicity, the detailed mechanism of toxicity in EP have not been clearly delineated. This study investigate the...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2021-06-01
|
Series: | Biomedicine & Pharmacotherapy |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332221003061 |
id |
doaj-07493eb5acd7425d813e58192e54ecb1 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei-ni Qin Kai-cheng Zhang Ting Geng Fang-fang Cheng Pei-dong Chen Wei-feng Yao Li Zhang Yu-dan Cao |
spellingShingle |
Wei-ni Qin Kai-cheng Zhang Ting Geng Fang-fang Cheng Pei-dong Chen Wei-feng Yao Li Zhang Yu-dan Cao The toxicity mechanism of toxic compounds from Euphorbiae pekinensis Radix on zebrafish embryos Biomedicine & Pharmacotherapy Euphorbiae pekinensis Vinegar-processing Toxicity Zebrafish embryos Apoptosis |
author_facet |
Wei-ni Qin Kai-cheng Zhang Ting Geng Fang-fang Cheng Pei-dong Chen Wei-feng Yao Li Zhang Yu-dan Cao |
author_sort |
Wei-ni Qin |
title |
The toxicity mechanism of toxic compounds from Euphorbiae pekinensis Radix on zebrafish embryos |
title_short |
The toxicity mechanism of toxic compounds from Euphorbiae pekinensis Radix on zebrafish embryos |
title_full |
The toxicity mechanism of toxic compounds from Euphorbiae pekinensis Radix on zebrafish embryos |
title_fullStr |
The toxicity mechanism of toxic compounds from Euphorbiae pekinensis Radix on zebrafish embryos |
title_full_unstemmed |
The toxicity mechanism of toxic compounds from Euphorbiae pekinensis Radix on zebrafish embryos |
title_sort |
toxicity mechanism of toxic compounds from euphorbiae pekinensis radix on zebrafish embryos |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2021-06-01 |
description |
Euphorbiae pekinensis Radix (EP) is effective in treating various diseases, but it’s toxicity is a major obstacle in use in clinical. Although EP was processed with vinegar to reduce it’s toxicity, the detailed mechanism of toxicity in EP have not been clearly delineated. This study investigate the toxicity attenuation-mechanism of Euphorbiae pekinensis after being processed with vinegar (VEP) and the toxic mechanism of four compounds from EP on zebrafish embryos. The contents of four compounds decreased obviously in VEP. Correspondingly, slower development on embryos can be seen as some symptoms like reduction of heart rate, liver area and gastrointestinal peristalsis after exposed to the compounds. Some obvious pathological signals such as pericardial edema and yolk sac edema were observed. Furthermore, the compounds could increase the contents of MDA and GSH-PX and induce oxidative damage by inhibiting the activity of SOD. Also, four compounds could provoke apoptosis by up-regulating the expression level of p53, MDM2, Bax, Bcl-2 and activating the activity of caspase-3, caspase-9. In conclusion, the four compounds play an important role in the toxicity attenuation effects of VEP, which may be related to the apoptosis induction and oxidative damage. This would contribute to the clinical application and further toxicity-reduction mechanism research. |
topic |
Euphorbiae pekinensis Vinegar-processing Toxicity Zebrafish embryos Apoptosis |
url |
http://www.sciencedirect.com/science/article/pii/S0753332221003061 |
work_keys_str_mv |
AT weiniqin thetoxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT kaichengzhang thetoxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT tinggeng thetoxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT fangfangcheng thetoxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT peidongchen thetoxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT weifengyao thetoxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT lizhang thetoxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT yudancao thetoxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT weiniqin toxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT kaichengzhang toxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT tinggeng toxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT fangfangcheng toxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT peidongchen toxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT weifengyao toxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT lizhang toxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos AT yudancao toxicitymechanismoftoxiccompoundsfromeuphorbiaepekinensisradixonzebrafishembryos |
_version_ |
1721434619208794112 |
spelling |
doaj-07493eb5acd7425d813e58192e54ecb12021-05-20T07:45:18ZengElsevierBiomedicine & Pharmacotherapy0753-33222021-06-01138111521The toxicity mechanism of toxic compounds from Euphorbiae pekinensis Radix on zebrafish embryosWei-ni Qin0Kai-cheng Zhang1Ting Geng2Fang-fang Cheng3Pei-dong Chen4Wei-feng Yao5Li Zhang6Yu-dan Cao7Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaNanjing University of Chinese Medicine Hanlin College, Taizhou 225300, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China; Correspondence to: Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing 210023, China.Euphorbiae pekinensis Radix (EP) is effective in treating various diseases, but it’s toxicity is a major obstacle in use in clinical. Although EP was processed with vinegar to reduce it’s toxicity, the detailed mechanism of toxicity in EP have not been clearly delineated. This study investigate the toxicity attenuation-mechanism of Euphorbiae pekinensis after being processed with vinegar (VEP) and the toxic mechanism of four compounds from EP on zebrafish embryos. The contents of four compounds decreased obviously in VEP. Correspondingly, slower development on embryos can be seen as some symptoms like reduction of heart rate, liver area and gastrointestinal peristalsis after exposed to the compounds. Some obvious pathological signals such as pericardial edema and yolk sac edema were observed. Furthermore, the compounds could increase the contents of MDA and GSH-PX and induce oxidative damage by inhibiting the activity of SOD. Also, four compounds could provoke apoptosis by up-regulating the expression level of p53, MDM2, Bax, Bcl-2 and activating the activity of caspase-3, caspase-9. In conclusion, the four compounds play an important role in the toxicity attenuation effects of VEP, which may be related to the apoptosis induction and oxidative damage. This would contribute to the clinical application and further toxicity-reduction mechanism research.http://www.sciencedirect.com/science/article/pii/S0753332221003061Euphorbiae pekinensisVinegar-processingToxicityZebrafish embryosApoptosis |