Detection and Measurement of the Intracellular Calcium Variation in Follicular Cells
This work presents a new method for measuring the variation of intracellular calcium in follicular cells. The proposal consists in two stages: (i) the detection of the cell’s nuclei and (ii) the analysis of the fluorescence variations. The first stage is performed via watershed modified transformati...
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Series: | Computational and Mathematical Methods in Medicine |
Online Access: | http://dx.doi.org/10.1155/2014/484656 |
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doaj-074b43d3e9114991820a9d9f01b970842020-11-24T20:59:39ZengHindawi LimitedComputational and Mathematical Methods in Medicine1748-670X1748-67182014-01-01201410.1155/2014/484656484656Detection and Measurement of the Intracellular Calcium Variation in Follicular CellsAna M. Herrera-Navarro0Iván R. Terol-Villalobos1Hugo Jiménez-Hernández2Hayde Peregrina-Barreto3José-Joel Gonzalez-Barboza4Facultad de Informática, Universidad Autónoma de Querétaro, Campus Juriquilla, Avenida de las Ciencias s/n, 76230 Querétaro, QRO, MexicoCentro de Investigación y Desarrollo Tecnológico en Electroquímica S.C., Pedro Escobedo, 76703 Querétaro, QRO, MexicoCentro de Investigación e Ingeniería Industrial. Avenida Playa Pie de la Cuesta No. 702, Desarrollo San Pablo, 76703 Querétaro, QRO, MexicoDepartamento de Ciencias Computacionales, Instituto Nacional de Astrofísica, Óptica y Electrónica, Luis Enrique Erro No. 1, CP 72840, Tonantzintla, PUE, MexicoCentro de Investigación y Tecnología Aplicada, Cerro Blanco No. 141, Colonia Colinas del Cimatario, 76090 Querétaro, QRO, MexicoThis work presents a new method for measuring the variation of intracellular calcium in follicular cells. The proposal consists in two stages: (i) the detection of the cell’s nuclei and (ii) the analysis of the fluorescence variations. The first stage is performed via watershed modified transformation, where the process of labeling is controlled. The detection process uses the contours of the cells as descriptors, where they are enhanced with a morphological filter that homogenizes the luminance variation of the image. In the second stage, the fluorescence variations are modeled as an exponential decreasing function, where the fluorescence variations are highly correlated with the changes of intracellular free Ca2+. Additionally, it is introduced a new morphological called medium reconstruction process, which helps to enhance the data for the modeling process. This filter exploits the undermodeling and overmodeling properties of reconstruction operators, such that it preserves the structure of the original signal. Finally, an experimental process shows evidence of the capabilities of the proposal.http://dx.doi.org/10.1155/2014/484656 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana M. Herrera-Navarro Iván R. Terol-Villalobos Hugo Jiménez-Hernández Hayde Peregrina-Barreto José-Joel Gonzalez-Barboza |
spellingShingle |
Ana M. Herrera-Navarro Iván R. Terol-Villalobos Hugo Jiménez-Hernández Hayde Peregrina-Barreto José-Joel Gonzalez-Barboza Detection and Measurement of the Intracellular Calcium Variation in Follicular Cells Computational and Mathematical Methods in Medicine |
author_facet |
Ana M. Herrera-Navarro Iván R. Terol-Villalobos Hugo Jiménez-Hernández Hayde Peregrina-Barreto José-Joel Gonzalez-Barboza |
author_sort |
Ana M. Herrera-Navarro |
title |
Detection and Measurement of the Intracellular Calcium Variation in Follicular Cells |
title_short |
Detection and Measurement of the Intracellular Calcium Variation in Follicular Cells |
title_full |
Detection and Measurement of the Intracellular Calcium Variation in Follicular Cells |
title_fullStr |
Detection and Measurement of the Intracellular Calcium Variation in Follicular Cells |
title_full_unstemmed |
Detection and Measurement of the Intracellular Calcium Variation in Follicular Cells |
title_sort |
detection and measurement of the intracellular calcium variation in follicular cells |
publisher |
Hindawi Limited |
series |
Computational and Mathematical Methods in Medicine |
issn |
1748-670X 1748-6718 |
publishDate |
2014-01-01 |
description |
This work presents a new method for measuring the variation of intracellular calcium in follicular cells. The proposal consists in two stages: (i) the detection of the cell’s nuclei and (ii) the analysis of the fluorescence variations. The first stage is performed via watershed modified transformation, where the process of labeling is controlled. The detection process uses the contours of the cells as descriptors, where they are enhanced with a morphological filter that homogenizes the luminance variation of the image. In the second stage, the fluorescence variations are modeled as an exponential decreasing function, where the fluorescence variations are highly correlated with the changes of intracellular free Ca2+. Additionally, it is introduced a new morphological called medium reconstruction process, which helps to enhance the data for the modeling process. This filter exploits the undermodeling and overmodeling properties of reconstruction operators, such that it preserves the structure of the original signal. Finally, an experimental process shows evidence of the capabilities of the proposal. |
url |
http://dx.doi.org/10.1155/2014/484656 |
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