Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway
As the number of patients with metabolic hypertension (MH) is increasing, there is an essential require for global measures to prevent and treat MH. Flavonoids such as buddleoside (BUD) from Chrysanthemum indicum L. are the main pharmacological components of cardiovascular activities. Previous studi...
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2021-10-01
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doaj-0766d90e474f42b7b3e3285c7b2402362021-10-08T10:50:40ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-10-011210.3389/fphar.2021.755140755140Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 PathwayYa-Jun Wang0Jie Su1Jing-Jing Yu2Mei-Qiu Yan3Meng-Lin Shi4Qi-Di Huang5Bo Li6Wen-Yan Wu7Rong-Shuang Xia8Si-Fan Li9Su-Hong Chen10Gui-Yuan Lv11School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, ChinaSchool of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, ChinaSchool of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, ChinaSchool of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, ChinaSchool of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, ChinaSchool of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, ChinaCollaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, ChinaSchool of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, ChinaSchool of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, ChinaSchool of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, ChinaCollaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, ChinaSchool of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, ChinaAs the number of patients with metabolic hypertension (MH) is increasing, there is an essential require for global measures to prevent and treat MH. Flavonoids such as buddleoside (BUD) from Chrysanthemum indicum L. are the main pharmacological components of cardiovascular activities. Previous studies have suggested that the buddleoside-rich Chrysanthemum indicum L. extract (BUDE) can reduce blood pressure in spontaneously hypertensive rats (SHR). However, its effect on MH and how it works remains to be researched. In this study, it was observed that BUDE could lower blood pressure, improve dyslipidemia, and decrease the level of plasma LPS in MH rats. Moreover, BUDE improved intestinal flora and increased the expression of occludin and claudin-1 in the colon, and improved the pathological injury of the colon. Western bolt and qRT-PCR experiments showed that BUDE could down-regulate TLR4 and MyD88 protein and mRNA expression and inhibit phosphorylation of IKKβ, IκBα and NF-κB p65 in vessels of MH rats. These results showed that BUDE could regulate intestinal flora, improve intestinal barrier function, reduce the production and penetration of LPS, thereby inhibiting the vascular TLR4/MyD88 pathway, improving vascular endothelial function, and ultimately lowering blood pressure in MH rats. This study provides a new mechanism of BUDE against MH by inhibiting the enteric-origin LPS/TLR4 pathway.https://www.frontiersin.org/articles/10.3389/fphar.2021.755140/fullmetabolic hypertensivebuddleosidelipopolysaccharideintestinal floraenteric-origin LPS/TLR4 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ya-Jun Wang Jie Su Jing-Jing Yu Mei-Qiu Yan Meng-Lin Shi Qi-Di Huang Bo Li Wen-Yan Wu Rong-Shuang Xia Si-Fan Li Su-Hong Chen Gui-Yuan Lv |
spellingShingle |
Ya-Jun Wang Jie Su Jing-Jing Yu Mei-Qiu Yan Meng-Lin Shi Qi-Di Huang Bo Li Wen-Yan Wu Rong-Shuang Xia Si-Fan Li Su-Hong Chen Gui-Yuan Lv Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway Frontiers in Pharmacology metabolic hypertensive buddleoside lipopolysaccharide intestinal flora enteric-origin LPS/TLR4 |
author_facet |
Ya-Jun Wang Jie Su Jing-Jing Yu Mei-Qiu Yan Meng-Lin Shi Qi-Di Huang Bo Li Wen-Yan Wu Rong-Shuang Xia Si-Fan Li Su-Hong Chen Gui-Yuan Lv |
author_sort |
Ya-Jun Wang |
title |
Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway |
title_short |
Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway |
title_full |
Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway |
title_fullStr |
Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway |
title_full_unstemmed |
Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway |
title_sort |
buddleoside-rich chrysanthemum indicum l. extract has a beneficial effect on metabolic hypertensive rats by inhibiting the enteric-origin lps/tlr4 pathway |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pharmacology |
issn |
1663-9812 |
publishDate |
2021-10-01 |
description |
As the number of patients with metabolic hypertension (MH) is increasing, there is an essential require for global measures to prevent and treat MH. Flavonoids such as buddleoside (BUD) from Chrysanthemum indicum L. are the main pharmacological components of cardiovascular activities. Previous studies have suggested that the buddleoside-rich Chrysanthemum indicum L. extract (BUDE) can reduce blood pressure in spontaneously hypertensive rats (SHR). However, its effect on MH and how it works remains to be researched. In this study, it was observed that BUDE could lower blood pressure, improve dyslipidemia, and decrease the level of plasma LPS in MH rats. Moreover, BUDE improved intestinal flora and increased the expression of occludin and claudin-1 in the colon, and improved the pathological injury of the colon. Western bolt and qRT-PCR experiments showed that BUDE could down-regulate TLR4 and MyD88 protein and mRNA expression and inhibit phosphorylation of IKKβ, IκBα and NF-κB p65 in vessels of MH rats. These results showed that BUDE could regulate intestinal flora, improve intestinal barrier function, reduce the production and penetration of LPS, thereby inhibiting the vascular TLR4/MyD88 pathway, improving vascular endothelial function, and ultimately lowering blood pressure in MH rats. This study provides a new mechanism of BUDE against MH by inhibiting the enteric-origin LPS/TLR4 pathway. |
topic |
metabolic hypertensive buddleoside lipopolysaccharide intestinal flora enteric-origin LPS/TLR4 |
url |
https://www.frontiersin.org/articles/10.3389/fphar.2021.755140/full |
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