Proteomic Analysis of Synovial Fibroblasts and Articular Chondrocytes Co-Cultures Reveals Valuable VIP-Modulated Inflammatory and Degradative Proteins in Osteoarthritis
Osteoarthritis (OA) is the most common musculoskeletal disorder causing a great disability and a reduction in the quality of life. In OA, articular chondrocytes (AC) and synovial fibroblasts (SF) release innate-derived immune mediators that initiate and perpetuate inflammation, inducing cartilage ex...
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doaj-079326b214f7493f93284eaf3a5ba8242021-07-01T00:19:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-01226441644110.3390/ijms22126441Proteomic Analysis of Synovial Fibroblasts and Articular Chondrocytes Co-Cultures Reveals Valuable VIP-Modulated Inflammatory and Degradative Proteins in OsteoarthritisSelene Pérez-García0Valentina Calamia1Tamara Hermida-Gómez2Irene Gutiérrez-Cañas3Mar Carrión4Raúl Villanueva-Romero5David Castro6Carmen Martínez7Yasmina Juarranz8Francisco J. Blanco9Rosa P. Gomariz10Department of Cell Biology, Faculty of Biology and Faculty of Medicine, Complutense University of Madrid (UCM), 28040 Madrid, SpainRheumatology Research Group, INIBIC-Biomedical Research Institute, CICA-University of A Coruña, 15006 A Coruña, SpainRheumatology Research Group, INIBIC-Biomedical Research Institute, CICA-University of A Coruña, 15006 A Coruña, SpainDepartment of Cell Biology, Faculty of Biology and Faculty of Medicine, Complutense University of Madrid (UCM), 28040 Madrid, SpainDepartment of Cell Biology, Faculty of Biology and Faculty of Medicine, Complutense University of Madrid (UCM), 28040 Madrid, SpainDepartment of Cell Biology, Faculty of Biology and Faculty of Medicine, Complutense University of Madrid (UCM), 28040 Madrid, SpainDepartment of Cell Biology, Faculty of Biology and Faculty of Medicine, Complutense University of Madrid (UCM), 28040 Madrid, SpainDepartment of Cell Biology, Faculty of Biology and Faculty of Medicine, Complutense University of Madrid (UCM), 28040 Madrid, SpainDepartment of Cell Biology, Faculty of Biology and Faculty of Medicine, Complutense University of Madrid (UCM), 28040 Madrid, SpainRheumatology Research Group, INIBIC-Biomedical Research Institute, CICA-University of A Coruña, 15006 A Coruña, SpainDepartment of Cell Biology, Faculty of Biology and Faculty of Medicine, Complutense University of Madrid (UCM), 28040 Madrid, SpainOsteoarthritis (OA) is the most common musculoskeletal disorder causing a great disability and a reduction in the quality of life. In OA, articular chondrocytes (AC) and synovial fibroblasts (SF) release innate-derived immune mediators that initiate and perpetuate inflammation, inducing cartilage extracellular matrix (ECM) degradation. Given the lack of therapies for the treatment of OA, in this study, we explore biomarkers that enable the development of new therapeutical approaches. We analyze the set of secreted proteins in AC and SF co-cultures by stable isotope labeling with amino acids (SILAC). We describe, for the first time, 115 proteins detected in SF-AC co-cultures stimulated by fibronectin fragments (Fn-fs). We also study the role of the vasoactive intestinal peptide (VIP) in this secretome, providing new proteins involved in the main events of OA, confirmed by ELISA and multiplex analyses. VIP decreases proteins involved in the inflammatory process (CHI3L1, PTX3), complement activation (C1r, C3), and cartilage ECM degradation (DCN, CTSB and MMP2), key events in the initiation and progression of OA. Our results support the anti-inflammatory and anti-catabolic properties of VIP in rheumatic diseases and provide potential new targets for OA treatment.https://www.mdpi.com/1422-0067/22/12/6441osteoarthritissynovial fibroblastschondrocytesVIPCHI3L1PTX3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Selene Pérez-García Valentina Calamia Tamara Hermida-Gómez Irene Gutiérrez-Cañas Mar Carrión Raúl Villanueva-Romero David Castro Carmen Martínez Yasmina Juarranz Francisco J. Blanco Rosa P. Gomariz |
spellingShingle |
Selene Pérez-García Valentina Calamia Tamara Hermida-Gómez Irene Gutiérrez-Cañas Mar Carrión Raúl Villanueva-Romero David Castro Carmen Martínez Yasmina Juarranz Francisco J. Blanco Rosa P. Gomariz Proteomic Analysis of Synovial Fibroblasts and Articular Chondrocytes Co-Cultures Reveals Valuable VIP-Modulated Inflammatory and Degradative Proteins in Osteoarthritis International Journal of Molecular Sciences osteoarthritis synovial fibroblasts chondrocytes VIP CHI3L1 PTX3 |
author_facet |
Selene Pérez-García Valentina Calamia Tamara Hermida-Gómez Irene Gutiérrez-Cañas Mar Carrión Raúl Villanueva-Romero David Castro Carmen Martínez Yasmina Juarranz Francisco J. Blanco Rosa P. Gomariz |
author_sort |
Selene Pérez-García |
title |
Proteomic Analysis of Synovial Fibroblasts and Articular Chondrocytes Co-Cultures Reveals Valuable VIP-Modulated Inflammatory and Degradative Proteins in Osteoarthritis |
title_short |
Proteomic Analysis of Synovial Fibroblasts and Articular Chondrocytes Co-Cultures Reveals Valuable VIP-Modulated Inflammatory and Degradative Proteins in Osteoarthritis |
title_full |
Proteomic Analysis of Synovial Fibroblasts and Articular Chondrocytes Co-Cultures Reveals Valuable VIP-Modulated Inflammatory and Degradative Proteins in Osteoarthritis |
title_fullStr |
Proteomic Analysis of Synovial Fibroblasts and Articular Chondrocytes Co-Cultures Reveals Valuable VIP-Modulated Inflammatory and Degradative Proteins in Osteoarthritis |
title_full_unstemmed |
Proteomic Analysis of Synovial Fibroblasts and Articular Chondrocytes Co-Cultures Reveals Valuable VIP-Modulated Inflammatory and Degradative Proteins in Osteoarthritis |
title_sort |
proteomic analysis of synovial fibroblasts and articular chondrocytes co-cultures reveals valuable vip-modulated inflammatory and degradative proteins in osteoarthritis |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-06-01 |
description |
Osteoarthritis (OA) is the most common musculoskeletal disorder causing a great disability and a reduction in the quality of life. In OA, articular chondrocytes (AC) and synovial fibroblasts (SF) release innate-derived immune mediators that initiate and perpetuate inflammation, inducing cartilage extracellular matrix (ECM) degradation. Given the lack of therapies for the treatment of OA, in this study, we explore biomarkers that enable the development of new therapeutical approaches. We analyze the set of secreted proteins in AC and SF co-cultures by stable isotope labeling with amino acids (SILAC). We describe, for the first time, 115 proteins detected in SF-AC co-cultures stimulated by fibronectin fragments (Fn-fs). We also study the role of the vasoactive intestinal peptide (VIP) in this secretome, providing new proteins involved in the main events of OA, confirmed by ELISA and multiplex analyses. VIP decreases proteins involved in the inflammatory process (CHI3L1, PTX3), complement activation (C1r, C3), and cartilage ECM degradation (DCN, CTSB and MMP2), key events in the initiation and progression of OA. Our results support the anti-inflammatory and anti-catabolic properties of VIP in rheumatic diseases and provide potential new targets for OA treatment. |
topic |
osteoarthritis synovial fibroblasts chondrocytes VIP CHI3L1 PTX3 |
url |
https://www.mdpi.com/1422-0067/22/12/6441 |
work_keys_str_mv |
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