miR-126 Is Involved in Vascular Remodeling under Laminar Shear Stress
Morphology and changes in gene expression of vascular endothelium are mainly due to shear stress and inflammation. Cell phenotype modulation has been clearly demonstrated to be controlled by small noncoding micro-RNAs (miRNAs). This study focused on the effect of laminar shear stress (LSS) on human...
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doaj-07960f1e817049a298bec36d2134d6302020-11-25T01:00:23ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/497280497280miR-126 Is Involved in Vascular Remodeling under Laminar Shear StressAna Mondadori dos Santos0Laurent Metzinger1Oualid Haddad2Eléonore M’baya-Moutoula3Fatiha Taïbi4Nathalie Charnaux5Ziad A. Massy6Hanna Hlawaty7Valérie Metzinger-Le Meuth8INSERM U1088, Faculty of Pharmacy and Medicine, University of Picardie Jules Verne, Rue des Louvels, 80037 Amiens, FranceINSERM U1088, Faculty of Pharmacy and Medicine, University of Picardie Jules Verne, Rue des Louvels, 80037 Amiens, FranceINSERM U1148, LVTS, UFR SMBH, University Paris 13 Sorbonne Paris Cité, 74 rue Marcel Cachin, 93000 Bobigny, FranceINSERM U1088, Faculty of Pharmacy and Medicine, University of Picardie Jules Verne, Rue des Louvels, 80037 Amiens, FranceINSERM U1088, Faculty of Pharmacy and Medicine, University of Picardie Jules Verne, Rue des Louvels, 80037 Amiens, FranceINSERM U1148, LVTS, UFR SMBH, University Paris 13 Sorbonne Paris Cité, 74 rue Marcel Cachin, 93000 Bobigny, FranceINSERM U1088, Faculty of Pharmacy and Medicine, University of Picardie Jules Verne, Rue des Louvels, 80037 Amiens, FranceINSERM U1148, LVTS, UFR SMBH, University Paris 13 Sorbonne Paris Cité, 74 rue Marcel Cachin, 93000 Bobigny, FranceINSERM U1088, Faculty of Pharmacy and Medicine, University of Picardie Jules Verne, Rue des Louvels, 80037 Amiens, FranceMorphology and changes in gene expression of vascular endothelium are mainly due to shear stress and inflammation. Cell phenotype modulation has been clearly demonstrated to be controlled by small noncoding micro-RNAs (miRNAs). This study focused on the effect of laminar shear stress (LSS) on human endothelial cells (HUVECs), with an emphasis on the role of miRNA-126 (miR-126). Exposure of HUVECs in vitro to LSS modified the shape of HUVECs and concomitantly regulated the expression of miR-126, vascular cell adhesion molecule 1 (VCAM-1), and syndecan-4 (SDC-4). A significant upregulation of miR-126 during long-term exposure to flow was shown. Interestingly, LSS enhanced SDC-4 expression on the HUVEC membranes. Overexpression of miR-126 in HUVECs decreased the levels of targets stromal cell-derived factor-1 SDF-1/CXCL12 and VCAM-1 but increased the expression of RGS16, CXCR4, and SDC-4. No significant difference in terms of cell proliferation and apoptosis was observed between scramble, anti-miR-126, and pre-miR-126 transfected HUVECs. In Apo-E KO/CKD mice aortas expressing a high level of miR-126, SDC-4 was concomitantly increased. In conclusion, our results suggest that miR-126 (i) is overexpressed by long-term LSS, (ii) has a role in up- and downregulation of genes involved in atherosclerosis, and (iii) affects SDC-4 expression.http://dx.doi.org/10.1155/2015/497280 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana Mondadori dos Santos Laurent Metzinger Oualid Haddad Eléonore M’baya-Moutoula Fatiha Taïbi Nathalie Charnaux Ziad A. Massy Hanna Hlawaty Valérie Metzinger-Le Meuth |
spellingShingle |
Ana Mondadori dos Santos Laurent Metzinger Oualid Haddad Eléonore M’baya-Moutoula Fatiha Taïbi Nathalie Charnaux Ziad A. Massy Hanna Hlawaty Valérie Metzinger-Le Meuth miR-126 Is Involved in Vascular Remodeling under Laminar Shear Stress BioMed Research International |
author_facet |
Ana Mondadori dos Santos Laurent Metzinger Oualid Haddad Eléonore M’baya-Moutoula Fatiha Taïbi Nathalie Charnaux Ziad A. Massy Hanna Hlawaty Valérie Metzinger-Le Meuth |
author_sort |
Ana Mondadori dos Santos |
title |
miR-126 Is Involved in Vascular Remodeling under Laminar Shear Stress |
title_short |
miR-126 Is Involved in Vascular Remodeling under Laminar Shear Stress |
title_full |
miR-126 Is Involved in Vascular Remodeling under Laminar Shear Stress |
title_fullStr |
miR-126 Is Involved in Vascular Remodeling under Laminar Shear Stress |
title_full_unstemmed |
miR-126 Is Involved in Vascular Remodeling under Laminar Shear Stress |
title_sort |
mir-126 is involved in vascular remodeling under laminar shear stress |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
Morphology and changes in gene expression of vascular endothelium are mainly due to shear stress and inflammation. Cell phenotype modulation has been clearly demonstrated to be controlled by small noncoding micro-RNAs (miRNAs). This study focused on the effect of laminar shear stress (LSS) on human endothelial cells (HUVECs), with an emphasis on the role of miRNA-126 (miR-126). Exposure of HUVECs in vitro to LSS modified the shape of HUVECs and concomitantly regulated the expression of miR-126, vascular cell adhesion molecule 1 (VCAM-1), and syndecan-4 (SDC-4). A significant upregulation of miR-126 during long-term exposure to flow was shown. Interestingly, LSS enhanced SDC-4 expression on the HUVEC membranes. Overexpression of miR-126 in HUVECs decreased the levels of targets stromal cell-derived factor-1 SDF-1/CXCL12 and VCAM-1 but increased the expression of RGS16, CXCR4, and SDC-4. No significant difference in terms of cell proliferation and apoptosis was observed between scramble, anti-miR-126, and pre-miR-126 transfected HUVECs. In Apo-E KO/CKD mice aortas expressing a high level of miR-126, SDC-4 was concomitantly increased. In conclusion, our results suggest that miR-126 (i) is overexpressed by long-term LSS, (ii) has a role in up- and downregulation of genes involved in atherosclerosis, and (iii) affects SDC-4 expression. |
url |
http://dx.doi.org/10.1155/2015/497280 |
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