Effect of Phenanthrene on the Tissue Structure of Liver and Aminotransferase Enzymes in Yellowfin Seabream (Acanthopagrus latus)

Background: Polycyclic aromatic hydrocarbons (PAHs) such as phenanthrene (Phe) represent one of the most abundant forms of organic pollutants. The aim of this study was to assess changes in plasma levels of aminotransferase enzymes, total protein and liver tissue as biomarkers of yellowfin seabream...

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Main Authors: Mehrnaz Shirmohammadi*, Negin Salamat, Mohammad Taghi Ronagh, Abdolali Movahedinia, Gholamreza Hamidian
Format: Article
Language:English
Published: Arak University of Medical Sciences 2017-06-01
Series:Iranian Journal of Toxicology
Subjects:
Online Access:http://ijt.arakmu.ac.ir/article-1-585-en.pdf
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spelling doaj-07a910d9a1894410839f9ca6758f7b8d2020-11-24T23:39:39ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592017-06-011143341Effect of Phenanthrene on the Tissue Structure of Liver and Aminotransferase Enzymes in Yellowfin Seabream (Acanthopagrus latus)Mehrnaz Shirmohammadi*0 Negin Salamat 1Mohammad Taghi Ronagh 2 Abdolali Movahedinia 3Gholamreza Hamidian 4Department of Marine Biology, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran. * Corresponding Author: E-mail: m.shirmohammadi@kmsu.ac.irDepartment of Marine Biology, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran. Department of Marine Biology, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran. Department of Marine Biology, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran. Department of Basic Sciences, University of Tabriz, Tabriz, Iran.Background: Polycyclic aromatic hydrocarbons (PAHs) such as phenanthrene (Phe) represent one of the most abundant forms of organic pollutants. The aim of this study was to assess changes in plasma levels of aminotransferase enzymes, total protein and liver tissue as biomarkers of yellowfin seabream (Acanthopagrus latus) exposed to Phe for 14 d. Methods: The research was carried out in January 2016 at Khorramshahr University of Marine Sciences and Technology, Khorramshahr, Iran. Some 72 fish were injected with 2, 20, 40 and 70 mg/kg of Phe. Then tissue and blood samples were obtained at 1, 4, 7 and 14 d after injection. Results: Exposure of fish to Phe resulted in a significant increase of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and decrease of total protein after 7 d of the experiment (P<0.05). The main histopathological alteration was showed in different sampling days including nucleus margination, hypertrophy, vacuolation, melanomacrophages aggregates, sinusoid dilation, degeneration and picnotic nucleus. Degree of tissue change (DTC) of liver was recorded in the Phe-exposed fish from normal range to moderate changes. Conclusion: The studied biomarkers such as changes in concentrations of ALT, AST and total protein as well as tissue damages in liver may be served as beneficial biomarker to assess Phe toxicity in yellowfin seabream. http://ijt.arakmu.ac.ir/article-1-585-en.pdfAminotransferase EnzymesLivePhenanthreneYellowfin Seabream
collection DOAJ
language English
format Article
sources DOAJ
author Mehrnaz Shirmohammadi*
Negin Salamat
Mohammad Taghi Ronagh
Abdolali Movahedinia
Gholamreza Hamidian
spellingShingle Mehrnaz Shirmohammadi*
Negin Salamat
Mohammad Taghi Ronagh
Abdolali Movahedinia
Gholamreza Hamidian
Effect of Phenanthrene on the Tissue Structure of Liver and Aminotransferase Enzymes in Yellowfin Seabream (Acanthopagrus latus)
Iranian Journal of Toxicology
Aminotransferase Enzymes
Live
Phenanthrene
Yellowfin Seabream
author_facet Mehrnaz Shirmohammadi*
Negin Salamat
Mohammad Taghi Ronagh
Abdolali Movahedinia
Gholamreza Hamidian
author_sort Mehrnaz Shirmohammadi*
title Effect of Phenanthrene on the Tissue Structure of Liver and Aminotransferase Enzymes in Yellowfin Seabream (Acanthopagrus latus)
title_short Effect of Phenanthrene on the Tissue Structure of Liver and Aminotransferase Enzymes in Yellowfin Seabream (Acanthopagrus latus)
title_full Effect of Phenanthrene on the Tissue Structure of Liver and Aminotransferase Enzymes in Yellowfin Seabream (Acanthopagrus latus)
title_fullStr Effect of Phenanthrene on the Tissue Structure of Liver and Aminotransferase Enzymes in Yellowfin Seabream (Acanthopagrus latus)
title_full_unstemmed Effect of Phenanthrene on the Tissue Structure of Liver and Aminotransferase Enzymes in Yellowfin Seabream (Acanthopagrus latus)
title_sort effect of phenanthrene on the tissue structure of liver and aminotransferase enzymes in yellowfin seabream (acanthopagrus latus)
publisher Arak University of Medical Sciences
series Iranian Journal of Toxicology
issn 2008-2967
2251-9459
publishDate 2017-06-01
description Background: Polycyclic aromatic hydrocarbons (PAHs) such as phenanthrene (Phe) represent one of the most abundant forms of organic pollutants. The aim of this study was to assess changes in plasma levels of aminotransferase enzymes, total protein and liver tissue as biomarkers of yellowfin seabream (Acanthopagrus latus) exposed to Phe for 14 d. Methods: The research was carried out in January 2016 at Khorramshahr University of Marine Sciences and Technology, Khorramshahr, Iran. Some 72 fish were injected with 2, 20, 40 and 70 mg/kg of Phe. Then tissue and blood samples were obtained at 1, 4, 7 and 14 d after injection. Results: Exposure of fish to Phe resulted in a significant increase of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and decrease of total protein after 7 d of the experiment (P<0.05). The main histopathological alteration was showed in different sampling days including nucleus margination, hypertrophy, vacuolation, melanomacrophages aggregates, sinusoid dilation, degeneration and picnotic nucleus. Degree of tissue change (DTC) of liver was recorded in the Phe-exposed fish from normal range to moderate changes. Conclusion: The studied biomarkers such as changes in concentrations of ALT, AST and total protein as well as tissue damages in liver may be served as beneficial biomarker to assess Phe toxicity in yellowfin seabream.
topic Aminotransferase Enzymes
Live
Phenanthrene
Yellowfin Seabream
url http://ijt.arakmu.ac.ir/article-1-585-en.pdf
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