FtsZ-Dependent Elongation of a Coccoid Bacterium
A mechanistic understanding of the determination and maintenance of the simplest bacterial cell shape, a sphere, remains elusive compared with that of more complex shapes. Cocci seem to lack a dedicated elongation machinery, and a spherical shape has been considered an evolutionary dead-end morpholo...
Main Authors: | , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
American Society for Microbiology
2016-09-01
|
Series: | mBio |
Online Access: | http://mbio.asm.org/cgi/content/full/7/5/e00908-16 |
id |
doaj-07c6d0a930ed4683b0705f8171eb1e3e |
---|---|
record_format |
Article |
spelling |
doaj-07c6d0a930ed4683b0705f8171eb1e3e2021-07-02T02:03:18ZengAmerican Society for MicrobiologymBio2150-75112016-09-0175e00908-1610.1128/mBio.00908-16FtsZ-Dependent Elongation of a Coccoid BacteriumAna R. PereiraJen HsinEwa KrólAndreia C. TavaresPierre FloresEgbert HoiczykNatalie NgAlex DajkovicYves V. BrunMichael S. VanNieuwenhzeTerry RoemerRut Carballido-LopezDirk-Jan ScheffersKerwyn Casey HuangMariana G. PinhoA mechanistic understanding of the determination and maintenance of the simplest bacterial cell shape, a sphere, remains elusive compared with that of more complex shapes. Cocci seem to lack a dedicated elongation machinery, and a spherical shape has been considered an evolutionary dead-end morphology, as a transition from a spherical to a rod-like shape has never been observed in bacteria. Here we show that a Staphylococcus aureus mutant (M5) expressing the ftsZG193D allele exhibits elongated cells. Molecular dynamics simulations and in vitro studies indicate that FtsZG193D filaments are more twisted and shorter than wild-type filaments. In vivo, M5 cell wall deposition is initiated asymmetrically, only on one side of the cell, and progresses into a helical pattern rather than into a constricting ring as in wild-type cells. This helical pattern of wall insertion leads to elongation, as in rod-shaped cells. Thus, structural flexibility of FtsZ filaments can result in an FtsZ-dependent mechanism for generating elongated cells from cocci.http://mbio.asm.org/cgi/content/full/7/5/e00908-16 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana R. Pereira Jen Hsin Ewa Król Andreia C. Tavares Pierre Flores Egbert Hoiczyk Natalie Ng Alex Dajkovic Yves V. Brun Michael S. VanNieuwenhze Terry Roemer Rut Carballido-Lopez Dirk-Jan Scheffers Kerwyn Casey Huang Mariana G. Pinho |
spellingShingle |
Ana R. Pereira Jen Hsin Ewa Król Andreia C. Tavares Pierre Flores Egbert Hoiczyk Natalie Ng Alex Dajkovic Yves V. Brun Michael S. VanNieuwenhze Terry Roemer Rut Carballido-Lopez Dirk-Jan Scheffers Kerwyn Casey Huang Mariana G. Pinho FtsZ-Dependent Elongation of a Coccoid Bacterium mBio |
author_facet |
Ana R. Pereira Jen Hsin Ewa Król Andreia C. Tavares Pierre Flores Egbert Hoiczyk Natalie Ng Alex Dajkovic Yves V. Brun Michael S. VanNieuwenhze Terry Roemer Rut Carballido-Lopez Dirk-Jan Scheffers Kerwyn Casey Huang Mariana G. Pinho |
author_sort |
Ana R. Pereira |
title |
FtsZ-Dependent Elongation of a Coccoid Bacterium |
title_short |
FtsZ-Dependent Elongation of a Coccoid Bacterium |
title_full |
FtsZ-Dependent Elongation of a Coccoid Bacterium |
title_fullStr |
FtsZ-Dependent Elongation of a Coccoid Bacterium |
title_full_unstemmed |
FtsZ-Dependent Elongation of a Coccoid Bacterium |
title_sort |
ftsz-dependent elongation of a coccoid bacterium |
publisher |
American Society for Microbiology |
series |
mBio |
issn |
2150-7511 |
publishDate |
2016-09-01 |
description |
A mechanistic understanding of the determination and maintenance of the simplest bacterial cell shape, a sphere, remains elusive compared with that of more complex shapes. Cocci seem to lack a dedicated elongation machinery, and a spherical shape has been considered an evolutionary dead-end morphology, as a transition from a spherical to a rod-like shape has never been observed in bacteria. Here we show that a Staphylococcus aureus mutant (M5) expressing the ftsZG193D allele exhibits elongated cells. Molecular dynamics simulations and in vitro studies indicate that FtsZG193D filaments are more twisted and shorter than wild-type filaments. In vivo, M5 cell wall deposition is initiated asymmetrically, only on one side of the cell, and progresses into a helical pattern rather than into a constricting ring as in wild-type cells. This helical pattern of wall insertion leads to elongation, as in rod-shaped cells. Thus, structural flexibility of FtsZ filaments can result in an FtsZ-dependent mechanism for generating elongated cells from cocci. |
url |
http://mbio.asm.org/cgi/content/full/7/5/e00908-16 |
work_keys_str_mv |
AT anarpereira ftszdependentelongationofacoccoidbacterium AT jenhsin ftszdependentelongationofacoccoidbacterium AT ewakrol ftszdependentelongationofacoccoidbacterium AT andreiactavares ftszdependentelongationofacoccoidbacterium AT pierreflores ftszdependentelongationofacoccoidbacterium AT egberthoiczyk ftszdependentelongationofacoccoidbacterium AT natalieng ftszdependentelongationofacoccoidbacterium AT alexdajkovic ftszdependentelongationofacoccoidbacterium AT yvesvbrun ftszdependentelongationofacoccoidbacterium AT michaelsvannieuwenhze ftszdependentelongationofacoccoidbacterium AT terryroemer ftszdependentelongationofacoccoidbacterium AT rutcarballidolopez ftszdependentelongationofacoccoidbacterium AT dirkjanscheffers ftszdependentelongationofacoccoidbacterium AT kerwyncaseyhuang ftszdependentelongationofacoccoidbacterium AT marianagpinho ftszdependentelongationofacoccoidbacterium |
_version_ |
1721343897340215296 |