Worm peptidomics
Bioactive peptides are present in all metazoan species where they orchestrate diverse functions. In the last decade, high-throughput approaches based on mass spectrometry helped the identification of endogenously occurring peptides in different species. We here review biochemical strategies to obtai...
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doaj-07ca29673c164be1910f0f581de1ba5f2020-11-24T22:34:41ZengElsevierEuPA Open Proteomics2212-96852014-06-013C28029010.1016/j.euprot.2014.04.005Worm peptidomicsSteven J. Husson0Ank Reumer1Liesbet Temmerman2Wouter De Haes3Liliane Schoofs4Inge Mertens5Geert Baggerman6Systemic Physiological & Ecotoxicological Research (SPHERE), Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, BelgiumFunctional Genomics and Proteomics, Department of Biology, KU Leuven, Naamsestraat 59, B-3000 Leuven, BelgiumFunctional Genomics and Proteomics, Department of Biology, KU Leuven, Naamsestraat 59, B-3000 Leuven, BelgiumFunctional Genomics and Proteomics, Department of Biology, KU Leuven, Naamsestraat 59, B-3000 Leuven, BelgiumFunctional Genomics and Proteomics, Department of Biology, KU Leuven, Naamsestraat 59, B-3000 Leuven, BelgiumCentre for Proteome Analysis and Mass Spectrometry (CFP-CeProMa), University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, BelgiumCentre for Proteome Analysis and Mass Spectrometry (CFP-CeProMa), University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, BelgiumBioactive peptides are present in all metazoan species where they orchestrate diverse functions. In the last decade, high-throughput approaches based on mass spectrometry helped the identification of endogenously occurring peptides in different species. We here review biochemical strategies to obtain sequence information of natural (non-tryptic) peptides in Caenorhabditis elegans and in the related nematodes Caenorhabditis briggsae and Ascaris suum with particular attention for sample preparation and methodology. In addition, we describe seven new C. elegans neuropeptides that we recently discovered by sequencing additional peptides. Finally, we explain how differential peptidomics approaches were used to characterize key neuropeptide processing enzymes.http://www.sciencedirect.com/science/article/pii/S2212968514000300Mass spectrometryFMRFamide-like peptideNeuropeptide-like proteinInsulinNeuropeptideProcessing enzyme |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Steven J. Husson Ank Reumer Liesbet Temmerman Wouter De Haes Liliane Schoofs Inge Mertens Geert Baggerman |
spellingShingle |
Steven J. Husson Ank Reumer Liesbet Temmerman Wouter De Haes Liliane Schoofs Inge Mertens Geert Baggerman Worm peptidomics EuPA Open Proteomics Mass spectrometry FMRFamide-like peptide Neuropeptide-like protein Insulin Neuropeptide Processing enzyme |
author_facet |
Steven J. Husson Ank Reumer Liesbet Temmerman Wouter De Haes Liliane Schoofs Inge Mertens Geert Baggerman |
author_sort |
Steven J. Husson |
title |
Worm peptidomics |
title_short |
Worm peptidomics |
title_full |
Worm peptidomics |
title_fullStr |
Worm peptidomics |
title_full_unstemmed |
Worm peptidomics |
title_sort |
worm peptidomics |
publisher |
Elsevier |
series |
EuPA Open Proteomics |
issn |
2212-9685 |
publishDate |
2014-06-01 |
description |
Bioactive peptides are present in all metazoan species where they orchestrate diverse functions. In the last decade, high-throughput approaches based on mass spectrometry helped the identification of endogenously occurring peptides in different species. We here review biochemical strategies to obtain sequence information of natural (non-tryptic) peptides in Caenorhabditis elegans and in the related nematodes Caenorhabditis briggsae and Ascaris suum with particular attention for sample preparation and methodology. In addition, we describe seven new C. elegans neuropeptides that we recently discovered by sequencing additional peptides. Finally, we explain how differential peptidomics approaches were used to characterize key neuropeptide processing enzymes. |
topic |
Mass spectrometry FMRFamide-like peptide Neuropeptide-like protein Insulin Neuropeptide Processing enzyme |
url |
http://www.sciencedirect.com/science/article/pii/S2212968514000300 |
work_keys_str_mv |
AT stevenjhusson wormpeptidomics AT ankreumer wormpeptidomics AT liesbettemmerman wormpeptidomics AT wouterdehaes wormpeptidomics AT lilianeschoofs wormpeptidomics AT ingemertens wormpeptidomics AT geertbaggerman wormpeptidomics |
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1725725998770552832 |