Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments
For almost forty years, N-(1-pyrene) iodoacetamide has been used to label actin at C374, but the mechanisms of the fluorescence changes are still unknown due to the lack of structural information. Here authors provide cryo-EM structures of actin filaments with N-1-pyrene conjugated to cysteine 374 a...
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| Format: | Article |
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Nature Publishing Group
2020-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-19762-1 |
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doaj-07da98c81c624041b587e3c353b401412021-05-11T08:43:26ZengNature Publishing GroupNature Communications2041-17232020-11-011111910.1038/s41467-020-19762-1Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filamentsSteven Z. Chou0Thomas D. Pollard1Department of Molecular Cellular and Developmental Biology, Yale University, PO Box 208103Department of Molecular Cellular and Developmental Biology, Yale University, PO Box 208103For almost forty years, N-(1-pyrene) iodoacetamide has been used to label actin at C374, but the mechanisms of the fluorescence changes are still unknown due to the lack of structural information. Here authors provide cryo-EM structures of actin filaments with N-1-pyrene conjugated to cysteine 374 and either ADP or ADP-phosphate in the active site.https://doi.org/10.1038/s41467-020-19762-1 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Steven Z. Chou Thomas D. Pollard |
| spellingShingle |
Steven Z. Chou Thomas D. Pollard Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments Nature Communications |
| author_facet |
Steven Z. Chou Thomas D. Pollard |
| author_sort |
Steven Z. Chou |
| title |
Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments |
| title_short |
Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments |
| title_full |
Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments |
| title_fullStr |
Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments |
| title_full_unstemmed |
Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments |
| title_sort |
cryo-electron microscopy structures of pyrene-labeled adp-pi- and adp-actin filaments |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2020-11-01 |
| description |
For almost forty years, N-(1-pyrene) iodoacetamide has been used to label actin at C374, but the mechanisms of the fluorescence changes are still unknown due to the lack of structural information. Here authors provide cryo-EM structures of actin filaments with N-1-pyrene conjugated to cysteine 374 and either ADP or ADP-phosphate in the active site. |
| url |
https://doi.org/10.1038/s41467-020-19762-1 |
| work_keys_str_mv |
AT stevenzchou cryoelectronmicroscopystructuresofpyrenelabeledadppiandadpactinfilaments AT thomasdpollard cryoelectronmicroscopystructuresofpyrenelabeledadppiandadpactinfilaments |
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