Andrographolide Inhibits Proliferation and Metastasis of SGC7901 Gastric Cancer Cells

To explore the mechanisms by which andrographolide inhibits gastric cancer cell proliferation and metastasis, we employed the gastric cell line SGC7901 to investigate the anticancer effects of andrographolide. The cell survival ratio, cell migration and invasion, cell cycle, apoptosis, and matrix me...

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Main Authors: Lei Dai, Gang Wang, Wensheng Pan
Format: Article
Language:English
Published: Hindawi Limited 2017-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2017/6242103
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spelling doaj-07e44ec043e248caa6dbd8ce00a286502020-11-24T23:11:34ZengHindawi LimitedBioMed Research International2314-61332314-61412017-01-01201710.1155/2017/62421036242103Andrographolide Inhibits Proliferation and Metastasis of SGC7901 Gastric Cancer CellsLei Dai0Gang Wang1Wensheng Pan2Department of Gastroenterology, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, ChinaDepartment of Gastroenterology, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, ChinaDepartment of Gastroenterology, The Second Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou, Zhejiang, ChinaTo explore the mechanisms by which andrographolide inhibits gastric cancer cell proliferation and metastasis, we employed the gastric cell line SGC7901 to investigate the anticancer effects of andrographolide. The cell survival ratio, cell migration and invasion, cell cycle, apoptosis, and matrix metalloproteinase activity were assessed. Moreover, western blotting and real-time PCR were used to examine the protein expression levels and the mRNA expression levels, respectively. The survival ratio of cells decreased with an increasing concentration of andrographolide in a dose-dependent manner. Consistent results were also obtained using an apoptosis assay, as detected by flow cytometry. The cell cycle was blocked at the G2/M2 phase by andrographolide treatment, and the proportion of cells arrested at G1/M was enhanced as the dose increased. Similarly, wound healing and Transwell assays showed reduced migration and invasion of the gastric cancer cells at various concentrations of andrographolide. Andrographolide can inhibit cell proliferation, invasion, and migration, block the cell cycle, and promote apoptosis in SGC7901 cells. The mechanisms may include upregulated expression of Timp-1/2, cyclin B1, p-Cdc2, Bax, and Bik and downregulated expression of MMP-2/9 and antiapoptosis protein Bcl-2.http://dx.doi.org/10.1155/2017/6242103
collection DOAJ
language English
format Article
sources DOAJ
author Lei Dai
Gang Wang
Wensheng Pan
spellingShingle Lei Dai
Gang Wang
Wensheng Pan
Andrographolide Inhibits Proliferation and Metastasis of SGC7901 Gastric Cancer Cells
BioMed Research International
author_facet Lei Dai
Gang Wang
Wensheng Pan
author_sort Lei Dai
title Andrographolide Inhibits Proliferation and Metastasis of SGC7901 Gastric Cancer Cells
title_short Andrographolide Inhibits Proliferation and Metastasis of SGC7901 Gastric Cancer Cells
title_full Andrographolide Inhibits Proliferation and Metastasis of SGC7901 Gastric Cancer Cells
title_fullStr Andrographolide Inhibits Proliferation and Metastasis of SGC7901 Gastric Cancer Cells
title_full_unstemmed Andrographolide Inhibits Proliferation and Metastasis of SGC7901 Gastric Cancer Cells
title_sort andrographolide inhibits proliferation and metastasis of sgc7901 gastric cancer cells
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2017-01-01
description To explore the mechanisms by which andrographolide inhibits gastric cancer cell proliferation and metastasis, we employed the gastric cell line SGC7901 to investigate the anticancer effects of andrographolide. The cell survival ratio, cell migration and invasion, cell cycle, apoptosis, and matrix metalloproteinase activity were assessed. Moreover, western blotting and real-time PCR were used to examine the protein expression levels and the mRNA expression levels, respectively. The survival ratio of cells decreased with an increasing concentration of andrographolide in a dose-dependent manner. Consistent results were also obtained using an apoptosis assay, as detected by flow cytometry. The cell cycle was blocked at the G2/M2 phase by andrographolide treatment, and the proportion of cells arrested at G1/M was enhanced as the dose increased. Similarly, wound healing and Transwell assays showed reduced migration and invasion of the gastric cancer cells at various concentrations of andrographolide. Andrographolide can inhibit cell proliferation, invasion, and migration, block the cell cycle, and promote apoptosis in SGC7901 cells. The mechanisms may include upregulated expression of Timp-1/2, cyclin B1, p-Cdc2, Bax, and Bik and downregulated expression of MMP-2/9 and antiapoptosis protein Bcl-2.
url http://dx.doi.org/10.1155/2017/6242103
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