Antiproliferative, antimicrobial, and antioxidant activity of Lavandula angustifolia Mill. essential oil
Introduction: We studied the chemical composition and antimicrobial, antioxidant, and antiproliferative activities of essential oils from flowers of Lavandula angustifolia grown in Southern Bosnia and Herzegovina. Methods: The chemical profile of essential oil was evaluated by means of gas chrom...
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doaj-07e96a00f5ba40fe9e7acaaaa3f38a9b2020-11-25T00:33:53ZengUniversity of SarajevoJournal of Health Sciences 2232-75761986-80492017-04-017110.17532/jhsci.2017.412Antiproliferative, antimicrobial, and antioxidant activity of Lavandula angustifolia Mill. essential oilHaris Niksic0Elvira Kovac-Besovic1Elma Omeragic2Samija Muratovic3Jasna Kusturica4Kemal Duric5Department of Pharmacognosy, Faculty of Pharmacy University of Sarajevo, Sarajevo, Bosnia and HerzegovinaDepartment of Pharmacognosy, Faculty of Pharmacy University of Sarajevo, Sarajevo, Bosnia and HerzegovinaDepartment of Pharmacognosy, Faculty of Pharmacy University of Sarajevo, Sarajevo, Bosnia and HerzegovinaDepartment of Pharmacognosy, Faculty of Pharmacy University of Sarajevo, Sarajevo, Bosnia and HerzegovinaDepartment of Pharmacology, Faculty of Medicine University of Sarajevo, Bosnia and HerzegovinaDepartment of Pharmacognosy, Faculty of Pharmacy University of Sarajevo, Sarajevo, Bosnia and Herzegovina Introduction: We studied the chemical composition and antimicrobial, antioxidant, and antiproliferative activities of essential oils from flowers of Lavandula angustifolia grown in Southern Bosnia and Herzegovina. Methods: The chemical profile of essential oil was evaluated by means of gas chromatography-mass spectrometry. Antimicrobial activity was tested against six bacterial strains. The antioxidant activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) test and the antiproliferative activity against three human cancer cell lines, MCF-7, NCI-H460, and MOLT-4, were investigated using 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide tests. Results: In L. angustifolia essential oil, monoterpene alcohols were the most represented class of volatiles (51.8%), including linalool, lavandulol, and terpinen-4-ol, α-terpineol as the major components, followed by monoterpene esters (22.6%). The most important antibacterial activity of essential oil was expressed on Gram-negative strains. Investigated essential oil was able to reduce DPPH radicals into the neutral DPPH-H form (inhibitory concentration 50% [IC50] = 0.421 mg/ml), and this activity was dose dependent. The essential oil showed significant antiproliferative activity against three cancer cell lines, MOLT-4, MCF-7, and NCI-H460 cells, with IC50 values of 17, 94, and 97 µg/ml, respectively. The result of the antiproliferative assay indicates that MOLT-4 cell line was the most sensitive to investigated essential oil. Conclusion: The results revealed that L. angustifolia essential oil may be important growth inhibitor against the microbes studied. It also possesses significant antioxidant activity and demonstrated excellent antiproliferative activity against MOLT-4 cells. https://www.jhsci.ba/ojs/index.php/jhsci/article/view/606Lavandula angustifoliaessential oilantimicrobial activityantioxidant |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Haris Niksic Elvira Kovac-Besovic Elma Omeragic Samija Muratovic Jasna Kusturica Kemal Duric |
spellingShingle |
Haris Niksic Elvira Kovac-Besovic Elma Omeragic Samija Muratovic Jasna Kusturica Kemal Duric Antiproliferative, antimicrobial, and antioxidant activity of Lavandula angustifolia Mill. essential oil Journal of Health Sciences Lavandula angustifolia essential oil antimicrobial activity antioxidant |
author_facet |
Haris Niksic Elvira Kovac-Besovic Elma Omeragic Samija Muratovic Jasna Kusturica Kemal Duric |
author_sort |
Haris Niksic |
title |
Antiproliferative, antimicrobial, and antioxidant activity of Lavandula angustifolia Mill. essential oil |
title_short |
Antiproliferative, antimicrobial, and antioxidant activity of Lavandula angustifolia Mill. essential oil |
title_full |
Antiproliferative, antimicrobial, and antioxidant activity of Lavandula angustifolia Mill. essential oil |
title_fullStr |
Antiproliferative, antimicrobial, and antioxidant activity of Lavandula angustifolia Mill. essential oil |
title_full_unstemmed |
Antiproliferative, antimicrobial, and antioxidant activity of Lavandula angustifolia Mill. essential oil |
title_sort |
antiproliferative, antimicrobial, and antioxidant activity of lavandula angustifolia mill. essential oil |
publisher |
University of Sarajevo |
series |
Journal of Health Sciences |
issn |
2232-7576 1986-8049 |
publishDate |
2017-04-01 |
description |
Introduction: We studied the chemical composition and antimicrobial, antioxidant, and antiproliferative activities of essential oils from flowers of Lavandula angustifolia grown in Southern Bosnia and Herzegovina.
Methods: The chemical profile of essential oil was evaluated by means of gas chromatography-mass spectrometry. Antimicrobial activity was tested against six bacterial strains. The antioxidant activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) test and the antiproliferative activity against three human cancer cell lines, MCF-7, NCI-H460, and MOLT-4, were investigated using 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide tests.
Results: In L. angustifolia essential oil, monoterpene alcohols were the most represented class of volatiles (51.8%), including linalool, lavandulol, and terpinen-4-ol, α-terpineol as the major components, followed by monoterpene esters (22.6%). The most important antibacterial activity of essential oil was expressed on Gram-negative strains. Investigated essential oil was able to reduce DPPH radicals into the neutral DPPH-H form (inhibitory concentration 50% [IC50] = 0.421 mg/ml), and this activity was dose dependent. The essential oil showed significant antiproliferative activity against three cancer cell lines, MOLT-4, MCF-7, and NCI-H460 cells, with IC50 values of 17, 94, and 97 µg/ml, respectively. The result of the antiproliferative assay indicates that MOLT-4 cell line was the most sensitive to investigated essential oil.
Conclusion: The results revealed that L. angustifolia essential oil may be important growth inhibitor against the microbes studied. It also possesses significant antioxidant activity and demonstrated excellent antiproliferative activity against MOLT-4 cells.
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topic |
Lavandula angustifolia essential oil antimicrobial activity antioxidant |
url |
https://www.jhsci.ba/ojs/index.php/jhsci/article/view/606 |
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