Distribution of Shiga toxin genes subtypes in B phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays
Background and Objectives: Shiga toxin-producing Escherichia coli (STEC) have emerged as human pathogens and con- tamination via animal origin has been a major public health concern. We compared the distribution of phylogenetic groups and prevalence of stx gene variants among the pathogenic strains...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Tehran University of Medical Sciences
2015-11-01
|
Series: | Iranian Journal of Microbiology |
Subjects: | |
Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/131 |
id |
doaj-07ebc79df0c146b687d221b6dca1868a |
---|---|
record_format |
Article |
spelling |
doaj-07ebc79df0c146b687d221b6dca1868a2020-12-02T06:11:00ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472015-11-0174Distribution of Shiga toxin genes subtypes in B phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arraysHamid Staji0Alfreda Tonelli1Abbas Javaheri-Vayeghan2Emad Changizi3Mohammad Reza Salimi-Bejestani4Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.Istituto Zooprofilattico Sperimentaledell'Abruzzo e del Molise " G. Caporale", Research and Development, Campo Boario, 64100 Teramo, Italy.Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran. Background and Objectives: Shiga toxin-producing Escherichia coli (STEC) have emerged as human pathogens and con- tamination via animal origin has been a major public health concern. We compared the distribution of phylogenetic groups and prevalence of stx gene variants among the pathogenic strains of Escherichia coli isolated from feces of diarrheatic calves in Tehran suburb farms. Materials and Methods: In this study we screened 140 diarrheatic calves (1-15 days old) for E. coli strains during a 3 months period of time. The isolated strains were grouped into different phylotypes according to the presence of chuA, yjaA and TSPE4.C2 genes. Then, the prevalence of stx gene subtypes was evaluated in the B phylotypes. Results: From diarrheatic calves, 51 bacterial isolates were biochemically identified as E. coli and 31 isolates out of 51 were considered B phylotype using DNA Microarray technology. Of these isolates, 20 contained stx a and stx b and one harbored all mentioned variants of stx genes except stx b. Conclusion: This study showed that in Tehran suburb, the B phylotype of E. coli is prevalent as a causative agent of diarrhea in calves and the prevalence of stx gene subtypes is dominant in comparison with other subtypes. Considering the possibility that these stx genes can be spread to other strains, bovine E. coli strains are an important source of stx genes for other strains and further study and surveillance seems to be required for the exact identification of virulence profile of E. coli phylotypes in different hosts. https://ijm.tums.ac.ir/index.php/ijm/article/view/131Escherichia colicalf diarrheaB1 phylotypeshiga-like toxin subtypesTehran suburb |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hamid Staji Alfreda Tonelli Abbas Javaheri-Vayeghan Emad Changizi Mohammad Reza Salimi-Bejestani |
spellingShingle |
Hamid Staji Alfreda Tonelli Abbas Javaheri-Vayeghan Emad Changizi Mohammad Reza Salimi-Bejestani Distribution of Shiga toxin genes subtypes in B phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays Iranian Journal of Microbiology Escherichia coli calf diarrhea B1 phylotype shiga-like toxin subtypes Tehran suburb |
author_facet |
Hamid Staji Alfreda Tonelli Abbas Javaheri-Vayeghan Emad Changizi Mohammad Reza Salimi-Bejestani |
author_sort |
Hamid Staji |
title |
Distribution of Shiga toxin genes subtypes in B phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays |
title_short |
Distribution of Shiga toxin genes subtypes in B phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays |
title_full |
Distribution of Shiga toxin genes subtypes in B phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays |
title_fullStr |
Distribution of Shiga toxin genes subtypes in B phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays |
title_full_unstemmed |
Distribution of Shiga toxin genes subtypes in B phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays |
title_sort |
distribution of shiga toxin genes subtypes in b phylotypes of escherichia coli isolated from calves suffering from diarrhea in tehran suburb using dna oligonucleotide arrays |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Microbiology |
issn |
2008-3289 2008-4447 |
publishDate |
2015-11-01 |
description |
Background and Objectives: Shiga toxin-producing Escherichia coli (STEC) have emerged as human pathogens and con- tamination via animal origin has been a major public health concern. We compared the distribution of phylogenetic groups and prevalence of stx gene variants among the pathogenic strains of Escherichia coli isolated from feces of diarrheatic calves in Tehran suburb farms.
Materials and Methods: In this study we screened 140 diarrheatic calves (1-15 days old) for E. coli strains during a 3 months period of time. The isolated strains were grouped into different phylotypes according to the presence of chuA, yjaA and TSPE4.C2 genes. Then, the prevalence of stx gene subtypes was evaluated in the B phylotypes.
Results: From diarrheatic calves, 51 bacterial isolates were biochemically identified as E. coli and 31 isolates out of 51 were considered B phylotype using DNA Microarray technology. Of these isolates, 20 contained stx a and stx b and one harbored all mentioned variants of stx genes except stx b.
Conclusion: This study showed that in Tehran suburb, the B phylotype of E. coli is prevalent as a causative agent of diarrhea in calves and the prevalence of stx gene subtypes is dominant in comparison with other subtypes. Considering the possibility that these stx genes can be spread to other strains, bovine E. coli strains are an important source of stx genes for other strains and further study and surveillance seems to be required for the exact identification of virulence profile of E. coli phylotypes in different hosts.
|
topic |
Escherichia coli calf diarrhea B1 phylotype shiga-like toxin subtypes Tehran suburb |
url |
https://ijm.tums.ac.ir/index.php/ijm/article/view/131 |
work_keys_str_mv |
AT hamidstaji distributionofshigatoxingenessubtypesinbphylotypesofescherichiacoliisolatedfromcalvessufferingfromdiarrheaintehransuburbusingdnaoligonucleotidearrays AT alfredatonelli distributionofshigatoxingenessubtypesinbphylotypesofescherichiacoliisolatedfromcalvessufferingfromdiarrheaintehransuburbusingdnaoligonucleotidearrays AT abbasjavaherivayeghan distributionofshigatoxingenessubtypesinbphylotypesofescherichiacoliisolatedfromcalvessufferingfromdiarrheaintehransuburbusingdnaoligonucleotidearrays AT emadchangizi distributionofshigatoxingenessubtypesinbphylotypesofescherichiacoliisolatedfromcalvessufferingfromdiarrheaintehransuburbusingdnaoligonucleotidearrays AT mohammadrezasalimibejestani distributionofshigatoxingenessubtypesinbphylotypesofescherichiacoliisolatedfromcalvessufferingfromdiarrheaintehransuburbusingdnaoligonucleotidearrays |
_version_ |
1724408747742199808 |