Small extracellular vesicles from human adipose-derived mesenchymal stromal cells: a potential promoter of fat graft survival
Abstract Background Small extracellular vesicles (sEVs) with genetic information secreted by cells play a crucial role in the cellular microenvironment. In this study, our purpose is to explore the characteristics of the small extracellular vesicles of human adipose-derived mesenchymal stromal cells...
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doaj-07f35382c0d64bfd95d5e3d3586e65f02021-05-09T11:09:36ZengBMCStem Cell Research & Therapy1757-65122021-05-0112111410.1186/s13287-021-02319-4Small extracellular vesicles from human adipose-derived mesenchymal stromal cells: a potential promoter of fat graft survivalAizhen Chen0Shijie Tang1Jiawei He2Xiangyu Li3Guohao Peng4Haoruo Zhang5Jinghua Chen6Liangwan Chen7Xiaosong Chen8Department of Plastic Surgery, Fujian Medical University Union HospitalDepartment of Plastic Surgery, Fujian Medical University Union HospitalDepartment of Plastic Surgery, Fujian Medical University Union HospitalDepartment of Plastic Surgery, Fujian Medical University Union HospitalDepartment of Plastic Surgery, Fujian Medical University Union HospitalDepartment of Plastic Surgery, Fujian Medical University Union HospitalDepartment of Pharmaceutical Analysis, the School of Pharmacy, Fujian Medical UniversityDepartment of Cardiac Surgery, Fujian Medical University Union HospitalDepartment of Plastic Surgery, Fujian Medical University Union HospitalAbstract Background Small extracellular vesicles (sEVs) with genetic information secreted by cells play a crucial role in the cellular microenvironment. In this study, our purpose is to explore the characteristics of the small extracellular vesicles of human adipose-derived mesenchymal stromal cells (hADMSC-sEVs) and studied the role of hADMSC-sEVs in improving the survival rate of grafted fat. Methods In the present study, we used the transmission electron microscopy, nano-tracking analysis, nanoflow surface protein analysis, and zeta potential value to identify sEVs. SEVs’ trajectory was traced dynamically to verify whether hADMSC-sEVs can be internalized into human umbilical vein endothelial cells (HUVECs) in vitro at different times. The angiogenic property of hADMSC-sEVs was observed by measuring the volume, weight, and histological analysis of the grafted fats in nude mouse models. Results Our research showed that the hADMSC-sEVs were sEVs with double-layer membrane structure and the diameter of which is within 30–150 nm. hADMSC-sEVs exert biological influence mainly through internalization into cells. Compared with the control group, the hADMSC-sEVs group had a significantly higher survival rate of grafted fat, morphological integrity, and a lower degree of inflammation and fibrosis. And immunohistochemistry showed that hADMSC-sEVs significantly increased the neovascularisation and the expression of CD34, VEGFR2, and Ki-67 in the graft tissue. Conclusions As a potential nanomaterial, hADMSC-sEVs have been explored in the field of cell-free application of stem cell technology. hADMSC-sEVs promoted the survival of grafted fats by promoting the formation of new blood vessels, which is another promising progress in the field of regenerative medicine. We believe that hADMSC-sEVs will have a broad application prospect in the field of regenerative medicine in the future.https://doi.org/10.1186/s13287-021-02319-4Small extracellular vesiclesHuman adipose-derived mesenchymal stromal cellsFat graftAngiogenesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aizhen Chen Shijie Tang Jiawei He Xiangyu Li Guohao Peng Haoruo Zhang Jinghua Chen Liangwan Chen Xiaosong Chen |
spellingShingle |
Aizhen Chen Shijie Tang Jiawei He Xiangyu Li Guohao Peng Haoruo Zhang Jinghua Chen Liangwan Chen Xiaosong Chen Small extracellular vesicles from human adipose-derived mesenchymal stromal cells: a potential promoter of fat graft survival Stem Cell Research & Therapy Small extracellular vesicles Human adipose-derived mesenchymal stromal cells Fat graft Angiogenesis |
author_facet |
Aizhen Chen Shijie Tang Jiawei He Xiangyu Li Guohao Peng Haoruo Zhang Jinghua Chen Liangwan Chen Xiaosong Chen |
author_sort |
Aizhen Chen |
title |
Small extracellular vesicles from human adipose-derived mesenchymal stromal cells: a potential promoter of fat graft survival |
title_short |
Small extracellular vesicles from human adipose-derived mesenchymal stromal cells: a potential promoter of fat graft survival |
title_full |
Small extracellular vesicles from human adipose-derived mesenchymal stromal cells: a potential promoter of fat graft survival |
title_fullStr |
Small extracellular vesicles from human adipose-derived mesenchymal stromal cells: a potential promoter of fat graft survival |
title_full_unstemmed |
Small extracellular vesicles from human adipose-derived mesenchymal stromal cells: a potential promoter of fat graft survival |
title_sort |
small extracellular vesicles from human adipose-derived mesenchymal stromal cells: a potential promoter of fat graft survival |
publisher |
BMC |
series |
Stem Cell Research & Therapy |
issn |
1757-6512 |
publishDate |
2021-05-01 |
description |
Abstract Background Small extracellular vesicles (sEVs) with genetic information secreted by cells play a crucial role in the cellular microenvironment. In this study, our purpose is to explore the characteristics of the small extracellular vesicles of human adipose-derived mesenchymal stromal cells (hADMSC-sEVs) and studied the role of hADMSC-sEVs in improving the survival rate of grafted fat. Methods In the present study, we used the transmission electron microscopy, nano-tracking analysis, nanoflow surface protein analysis, and zeta potential value to identify sEVs. SEVs’ trajectory was traced dynamically to verify whether hADMSC-sEVs can be internalized into human umbilical vein endothelial cells (HUVECs) in vitro at different times. The angiogenic property of hADMSC-sEVs was observed by measuring the volume, weight, and histological analysis of the grafted fats in nude mouse models. Results Our research showed that the hADMSC-sEVs were sEVs with double-layer membrane structure and the diameter of which is within 30–150 nm. hADMSC-sEVs exert biological influence mainly through internalization into cells. Compared with the control group, the hADMSC-sEVs group had a significantly higher survival rate of grafted fat, morphological integrity, and a lower degree of inflammation and fibrosis. And immunohistochemistry showed that hADMSC-sEVs significantly increased the neovascularisation and the expression of CD34, VEGFR2, and Ki-67 in the graft tissue. Conclusions As a potential nanomaterial, hADMSC-sEVs have been explored in the field of cell-free application of stem cell technology. hADMSC-sEVs promoted the survival of grafted fats by promoting the formation of new blood vessels, which is another promising progress in the field of regenerative medicine. We believe that hADMSC-sEVs will have a broad application prospect in the field of regenerative medicine in the future. |
topic |
Small extracellular vesicles Human adipose-derived mesenchymal stromal cells Fat graft Angiogenesis |
url |
https://doi.org/10.1186/s13287-021-02319-4 |
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