Construction of Epstein-Bar virus cocktail peptide fused with Fcγ of IgG: as a potential delivery system for vaccine development
Epstein–Barr virus (EBV) associated with several diseases such as contagious mononucleosis chronic active EBV infection, and diverse sorts of malignant tumors. Therefore, using applicable vaccines could be advantageous for public health. Yet, the vaccine has been unavailable to protect from EBV so f...
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doaj-080f269571eb49d39180f932786d117a2020-11-25T02:06:06ZengTaylor & Francis GroupBioengineered2165-59792165-59872019-01-0110168969610.1080/21655979.2019.16943881694388Construction of Epstein-Bar virus cocktail peptide fused with Fcγ of IgG: as a potential delivery system for vaccine developmentSaeid Amel Jamehdar0Samira Tabaei1Baratali Mashkani2Reza Karimi3Morteza Motallebnezhad4Arezoo Esmaili5Mashhad University of Medical ScienceShiraz University of Medical SciencesMashhad University of Medical SciencesIslamic Azad UniversityTabriz University of Medical SciencesIslamic Azad UniversityEpstein–Barr virus (EBV) associated with several diseases such as contagious mononucleosis chronic active EBV infection, and diverse sorts of malignant tumors. Therefore, using applicable vaccines could be advantageous for public health. Yet, the vaccine has been unavailable to protect from EBV so far. In the current study, to develop a multi-peptide vaccine for EBV and assess its expression in Pichia pastoris yeast system, three immunodominant sequences in glycoprotein (gp) 85, gp350 and latent membrane protein 1 (LMP1) were chosen. To construct fusion peptide, -GGGGS- liker was applied. After cloning the fusion peptide in the pPICZαA expression vector, this recombinant vector processed and transfected into Pichia pastoris host cells. The expression of high level of EBV fusion peptide was confirmed by dot blot and SDS-PAGE procedures. The Pichia pastoris is capable of supporting EBV fusion peptide expression. The application of this fusion peptide as a peptide vaccine to fight EBV is suggested.http://dx.doi.org/10.1080/21655979.2019.1694388epstein-bar viruspichia pastorispeptide vaccinefusion peptide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Saeid Amel Jamehdar Samira Tabaei Baratali Mashkani Reza Karimi Morteza Motallebnezhad Arezoo Esmaili |
spellingShingle |
Saeid Amel Jamehdar Samira Tabaei Baratali Mashkani Reza Karimi Morteza Motallebnezhad Arezoo Esmaili Construction of Epstein-Bar virus cocktail peptide fused with Fcγ of IgG: as a potential delivery system for vaccine development Bioengineered epstein-bar virus pichia pastoris peptide vaccine fusion peptide |
author_facet |
Saeid Amel Jamehdar Samira Tabaei Baratali Mashkani Reza Karimi Morteza Motallebnezhad Arezoo Esmaili |
author_sort |
Saeid Amel Jamehdar |
title |
Construction of Epstein-Bar virus cocktail peptide fused with Fcγ of IgG: as a potential delivery system for vaccine development |
title_short |
Construction of Epstein-Bar virus cocktail peptide fused with Fcγ of IgG: as a potential delivery system for vaccine development |
title_full |
Construction of Epstein-Bar virus cocktail peptide fused with Fcγ of IgG: as a potential delivery system for vaccine development |
title_fullStr |
Construction of Epstein-Bar virus cocktail peptide fused with Fcγ of IgG: as a potential delivery system for vaccine development |
title_full_unstemmed |
Construction of Epstein-Bar virus cocktail peptide fused with Fcγ of IgG: as a potential delivery system for vaccine development |
title_sort |
construction of epstein-bar virus cocktail peptide fused with fcγ of igg: as a potential delivery system for vaccine development |
publisher |
Taylor & Francis Group |
series |
Bioengineered |
issn |
2165-5979 2165-5987 |
publishDate |
2019-01-01 |
description |
Epstein–Barr virus (EBV) associated with several diseases such as contagious mononucleosis chronic active EBV infection, and diverse sorts of malignant tumors. Therefore, using applicable vaccines could be advantageous for public health. Yet, the vaccine has been unavailable to protect from EBV so far. In the current study, to develop a multi-peptide vaccine for EBV and assess its expression in Pichia pastoris yeast system, three immunodominant sequences in glycoprotein (gp) 85, gp350 and latent membrane protein 1 (LMP1) were chosen. To construct fusion peptide, -GGGGS- liker was applied. After cloning the fusion peptide in the pPICZαA expression vector, this recombinant vector processed and transfected into Pichia pastoris host cells. The expression of high level of EBV fusion peptide was confirmed by dot blot and SDS-PAGE procedures. The Pichia pastoris is capable of supporting EBV fusion peptide expression. The application of this fusion peptide as a peptide vaccine to fight EBV is suggested. |
topic |
epstein-bar virus pichia pastoris peptide vaccine fusion peptide |
url |
http://dx.doi.org/10.1080/21655979.2019.1694388 |
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