Development and Validation of Spectrophotometric Methods for the Determination of Rasagiline in Pharmaceutical Preparations
This study presents three simple, rapid, and accurate spectrophotometric methods for the determination of Rasagiline (RSG) in pharmaceutical preparations. The determination procedures depend on the reaction of RSG with chloranilic acid for method A, tetrachloro-1,4-benzoquinone for method B, and 7,7...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2013-01-01
|
Series: | Journal of Spectroscopy |
Online Access: | http://dx.doi.org/10.1155/2013/919180 |
id |
doaj-0818b9503ae744a385c9ae9b4dfda10b |
---|---|
record_format |
Article |
spelling |
doaj-0818b9503ae744a385c9ae9b4dfda10b2020-11-24T22:21:06ZengHindawi LimitedJournal of Spectroscopy2314-49202314-49392013-01-01201310.1155/2013/919180919180Development and Validation of Spectrophotometric Methods for the Determination of Rasagiline in Pharmaceutical PreparationsSerife Evrim Kepekci Tekkeli0Armağan Önal1Fatemeh Bahadori2Bezmialem Vakif University, Faculty of Pharmacy, Department of Analytical Chemistry, Fatih, 34093 Istanbul, TurkeyIstanbul University, Faculty of Pharmacy, Department of Analytical Chemistry, Beyazit, 34116 Istanbul, TurkeyBezmialem Vakif University, Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Fatih, 34093 Istanbul, TurkeyThis study presents three simple, rapid, and accurate spectrophotometric methods for the determination of Rasagiline (RSG) in pharmaceutical preparations. The determination procedures depend on the reaction of RSG with chloranilic acid for method A, tetrachloro-1,4-benzoquinone for method B, and 7,7,8,8-tetracyanoquinodimethane for method C. The colored products were quantitated spectrophotometrically at 524, 535, and 843 nm for methods A, B, and C, respectively. Different variables affecting the reaction were optimized. Linearity ranges of the methods with good correlation coefficients (0.9988–0.9996) were observed as 25–300 µg mL−1, 25–350 µg mL−1, and 50–500 µg mL−1 for methods A, B, and C, respectively. The formation of products takes place through different mechanisms. The sites of interaction were confirmed by elemental analysis using IR and 1H-NMR spectroscopy. The validation of the methods was carried out in terms of specificity, linearity, accuracy, precision, robustness, limit of detection, and limit of quantitation. No interference was observed from concomitants usually present in dosage forms. The methods were applied successfully to the determination of RSG in pharmaceutical preparations.http://dx.doi.org/10.1155/2013/919180 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Serife Evrim Kepekci Tekkeli Armağan Önal Fatemeh Bahadori |
spellingShingle |
Serife Evrim Kepekci Tekkeli Armağan Önal Fatemeh Bahadori Development and Validation of Spectrophotometric Methods for the Determination of Rasagiline in Pharmaceutical Preparations Journal of Spectroscopy |
author_facet |
Serife Evrim Kepekci Tekkeli Armağan Önal Fatemeh Bahadori |
author_sort |
Serife Evrim Kepekci Tekkeli |
title |
Development and Validation of Spectrophotometric Methods for the Determination of Rasagiline in Pharmaceutical Preparations |
title_short |
Development and Validation of Spectrophotometric Methods for the Determination of Rasagiline in Pharmaceutical Preparations |
title_full |
Development and Validation of Spectrophotometric Methods for the Determination of Rasagiline in Pharmaceutical Preparations |
title_fullStr |
Development and Validation of Spectrophotometric Methods for the Determination of Rasagiline in Pharmaceutical Preparations |
title_full_unstemmed |
Development and Validation of Spectrophotometric Methods for the Determination of Rasagiline in Pharmaceutical Preparations |
title_sort |
development and validation of spectrophotometric methods for the determination of rasagiline in pharmaceutical preparations |
publisher |
Hindawi Limited |
series |
Journal of Spectroscopy |
issn |
2314-4920 2314-4939 |
publishDate |
2013-01-01 |
description |
This study presents three simple, rapid, and accurate spectrophotometric methods for the determination of Rasagiline (RSG) in pharmaceutical preparations. The determination procedures depend on the reaction of RSG with chloranilic acid for method A, tetrachloro-1,4-benzoquinone for method B, and 7,7,8,8-tetracyanoquinodimethane for method C. The colored products were quantitated spectrophotometrically at 524, 535, and 843 nm for methods A, B, and C, respectively. Different variables affecting the reaction were optimized. Linearity ranges of the methods with good correlation coefficients (0.9988–0.9996) were observed as 25–300 µg mL−1, 25–350 µg mL−1, and 50–500 µg mL−1 for methods A, B, and C, respectively. The formation of products takes place through different mechanisms. The sites of interaction were confirmed by elemental analysis using IR and 1H-NMR spectroscopy. The validation of the methods was carried out in terms of specificity, linearity, accuracy, precision, robustness, limit of detection, and limit of quantitation. No interference was observed from concomitants usually present in dosage forms. The methods were applied successfully to the determination of RSG in pharmaceutical preparations. |
url |
http://dx.doi.org/10.1155/2013/919180 |
work_keys_str_mv |
AT serifeevrimkepekcitekkeli developmentandvalidationofspectrophotometricmethodsforthedeterminationofrasagilineinpharmaceuticalpreparations AT armaganonal developmentandvalidationofspectrophotometricmethodsforthedeterminationofrasagilineinpharmaceuticalpreparations AT fatemehbahadori developmentandvalidationofspectrophotometricmethodsforthedeterminationofrasagilineinpharmaceuticalpreparations |
_version_ |
1725772252088107008 |