Magnetron-sputtered Polytetrafluoroethylene-stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence
Surface-enhanced fluorescence (SEF) requires the absorption/emission band of the fluorophore, the localized surface plasmon resonance (LSPR) of the nanostructure and the excitation wavelength to fall in the same (or very close) spectral range. In this paper, we monitor the SEF intensity and lifetime...
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doaj-0831c74c4ac84106975fef282a92499f2020-11-25T02:33:27ZengMDPI AGNanomaterials2079-49912020-04-011077377310.3390/nano10040773Magnetron-sputtered Polytetrafluoroethylene-stabilized Silver Nanoisland Surface for Surface-Enhanced FluorescenceMartin Šubr0Petr Praus1Eva Kočišová2Marek Procházka3Josef Štěpánek4Anna Kuzminova5Ondřej Kylián6Franck Sureau7Institute of Physics, Faculty of Mathematics and Physics, Charles University, Ke Karlovu 5, 121 16 Prague, Czech RepublicInstitute of Physics, Faculty of Mathematics and Physics, Charles University, Ke Karlovu 5, 121 16 Prague, Czech RepublicInstitute of Physics, Faculty of Mathematics and Physics, Charles University, Ke Karlovu 5, 121 16 Prague, Czech RepublicInstitute of Physics, Faculty of Mathematics and Physics, Charles University, Ke Karlovu 5, 121 16 Prague, Czech RepublicInstitute of Physics, Faculty of Mathematics and Physics, Charles University, Ke Karlovu 5, 121 16 Prague, Czech RepublicDepartment of Macromolecular Physics, Faculty of Mathematics and Physics, Charles University, V Holešovičkách 2, 180 00 Prague, Czech RepublicDepartment of Macromolecular Physics, Faculty of Mathematics and Physics, Charles University, V Holešovičkách 2, 180 00 Prague, Czech RepublicLaboratoire Jean Perrin, Sorbonne University, Case Courrier 114, 4 Place Jussieu, 75005 Paris, FranceSurface-enhanced fluorescence (SEF) requires the absorption/emission band of the fluorophore, the localized surface plasmon resonance (LSPR) of the nanostructure and the excitation wavelength to fall in the same (or very close) spectral range. In this paper, we monitor the SEF intensity and lifetime dependence of riboflavin (vitamin B2) adsorbed on a spacer-modified Ag substrate with respect to the thickness of the spacer. The substrates were formed by silver nanoislands deposited onto magnetron-sputtered polytetrafluoroethylene (ms-PTFE). The spacer was formed by the ms-PTFE layer with the thickness ranging from ~5 to 25 nm. The riboflavin dissolved in dimethylsulfoxide (DMSO) at a 10 µM concentration forms, at the ms-PTFE surface, a homogeneous layer of adsorbed molecules corresponding to a monomolecular layer. The microspectroscopic measurements of the adsorbed layer were performed through a sessile droplet; our study has shown the advantages and limitations of this approach. Time-resolved fluorescence enabled us to determine the enhanced fluorescence quantum yield due to the shortening of the radiative decay in the vicinity of the plasmonic surface. For the 5 nm ms-PTFE layer possessing the largest (estimated 4×) fluorescence enhancement, the quantum yield was increased 2.3×.https://www.mdpi.com/2079-4991/10/4/773surface-enhanced fluorescence (SEF)time-resolvedriboflavinlifetimeenhancement factor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Martin Šubr Petr Praus Eva Kočišová Marek Procházka Josef Štěpánek Anna Kuzminova Ondřej Kylián Franck Sureau |
spellingShingle |
Martin Šubr Petr Praus Eva Kočišová Marek Procházka Josef Štěpánek Anna Kuzminova Ondřej Kylián Franck Sureau Magnetron-sputtered Polytetrafluoroethylene-stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence Nanomaterials surface-enhanced fluorescence (SEF) time-resolved riboflavin lifetime enhancement factor |
author_facet |
Martin Šubr Petr Praus Eva Kočišová Marek Procházka Josef Štěpánek Anna Kuzminova Ondřej Kylián Franck Sureau |
author_sort |
Martin Šubr |
title |
Magnetron-sputtered Polytetrafluoroethylene-stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence |
title_short |
Magnetron-sputtered Polytetrafluoroethylene-stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence |
title_full |
Magnetron-sputtered Polytetrafluoroethylene-stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence |
title_fullStr |
Magnetron-sputtered Polytetrafluoroethylene-stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence |
title_full_unstemmed |
Magnetron-sputtered Polytetrafluoroethylene-stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence |
title_sort |
magnetron-sputtered polytetrafluoroethylene-stabilized silver nanoisland surface for surface-enhanced fluorescence |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2020-04-01 |
description |
Surface-enhanced fluorescence (SEF) requires the absorption/emission band of the fluorophore, the localized surface plasmon resonance (LSPR) of the nanostructure and the excitation wavelength to fall in the same (or very close) spectral range. In this paper, we monitor the SEF intensity and lifetime dependence of riboflavin (vitamin B2) adsorbed on a spacer-modified Ag substrate with respect to the thickness of the spacer. The substrates were formed by silver nanoislands deposited onto magnetron-sputtered polytetrafluoroethylene (ms-PTFE). The spacer was formed by the ms-PTFE layer with the thickness ranging from ~5 to 25 nm. The riboflavin dissolved in dimethylsulfoxide (DMSO) at a 10 µM concentration forms, at the ms-PTFE surface, a homogeneous layer of adsorbed molecules corresponding to a monomolecular layer. The microspectroscopic measurements of the adsorbed layer were performed through a sessile droplet; our study has shown the advantages and limitations of this approach. Time-resolved fluorescence enabled us to determine the enhanced fluorescence quantum yield due to the shortening of the radiative decay in the vicinity of the plasmonic surface. For the 5 nm ms-PTFE layer possessing the largest (estimated 4×) fluorescence enhancement, the quantum yield was increased 2.3×. |
topic |
surface-enhanced fluorescence (SEF) time-resolved riboflavin lifetime enhancement factor |
url |
https://www.mdpi.com/2079-4991/10/4/773 |
work_keys_str_mv |
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