The IL-15 / sIL-15Rα complex modulates immunity without effect on asthma features in mouse
Abstract Background Interleukin 15 (IL-15) is a growth and modulating factor for B, T lymphocytes and natural killer cells (NK). Its action on innate and adaptive immunity is modulated by its alpha chain receptor (IL-15Rα). The IL-15/sIL-15Rα complex (IL-15Cx) increases the bioavailability and activ...
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doaj-083ab576a77a4fc39c35f105a880adfb2021-01-31T16:06:14ZengBMCRespiratory Research1465-993X2020-01-0121111010.1186/s12931-020-1301-xThe IL-15 / sIL-15Rα complex modulates immunity without effect on asthma features in mouseAntoine Moui0Martin Klein1Dorian Hassoun2Eléonore Dijoux3Marie-Aude Cheminant4Antoine Magnan5Grégory Bouchaud6L’institut du thorax, Inserm, CNRS, Université́ de NantesL’institut du thorax, Inserm, CNRS, Université́ de NantesL’institut du thorax, Inserm, CNRS, Université́ de NantesL’institut du thorax, Inserm, CNRS, Université́ de NantesL’institut du thorax, Inserm, CNRS, Université́ de NantesL’institut du thorax, Inserm, CNRS, Université́ de NantesINRA, UR1268 BIA, rue de la GéraudièreAbstract Background Interleukin 15 (IL-15) is a growth and modulating factor for B, T lymphocytes and natural killer cells (NK). Its action on innate and adaptive immunity is modulated by its alpha chain receptor (IL-15Rα). The IL-15/sIL-15Rα complex (IL-15Cx) increases the bioavailability and activity of the cytokine in vivo. IL-15Cx has been used in diseases to dampen IL-15 inflammation by the use of soluble IL-15Ralpha specificity. Here, we aim to evaluate the interest of IL-15Cx in a mouse model of asthma. Methods Using a mouse model of asthma consisting in percutaneous sensitization and intranasal challenge with total house dust mite extract, we evaluated the effect of IL-15Cx injected intraperitoneally four times after a first nasal challenge. Respiratory function was assessed by the technique of forced oscillations (Flexivent®). The effect on bronchial remodeling was evaluated by lung histology. The inflammatory status was analyzed by flow cytometry. Results We observed that the IL-15Cx modulates lung and systemic inflammation by increasing NK cells, CD8+ memory T cells and regulatory cells. However, IL-15Cx displays no effect on bronchial hyperreactivity, bronchial remodeling nor cellular bronchial infiltrate, but limits the secretion of bronchial mucus and modulates only inflammatory response in a HDM-allergic asthma murine model. Conclusions IL-15Cx has a limited effect on immune response in asthma and has no effect on lung function in mice. Thus, it limits its therapeutic potential but might suggest a combinatory potential with other therapeutics.https://doi.org/10.1186/s12931-020-1301-xInterleukin-15Interleukin-15 complexAsthmaAllergy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Antoine Moui Martin Klein Dorian Hassoun Eléonore Dijoux Marie-Aude Cheminant Antoine Magnan Grégory Bouchaud |
spellingShingle |
Antoine Moui Martin Klein Dorian Hassoun Eléonore Dijoux Marie-Aude Cheminant Antoine Magnan Grégory Bouchaud The IL-15 / sIL-15Rα complex modulates immunity without effect on asthma features in mouse Respiratory Research Interleukin-15 Interleukin-15 complex Asthma Allergy |
author_facet |
Antoine Moui Martin Klein Dorian Hassoun Eléonore Dijoux Marie-Aude Cheminant Antoine Magnan Grégory Bouchaud |
author_sort |
Antoine Moui |
title |
The IL-15 / sIL-15Rα complex modulates immunity without effect on asthma features in mouse |
title_short |
The IL-15 / sIL-15Rα complex modulates immunity without effect on asthma features in mouse |
title_full |
The IL-15 / sIL-15Rα complex modulates immunity without effect on asthma features in mouse |
title_fullStr |
The IL-15 / sIL-15Rα complex modulates immunity without effect on asthma features in mouse |
title_full_unstemmed |
The IL-15 / sIL-15Rα complex modulates immunity without effect on asthma features in mouse |
title_sort |
il-15 / sil-15rα complex modulates immunity without effect on asthma features in mouse |
publisher |
BMC |
series |
Respiratory Research |
issn |
1465-993X |
publishDate |
2020-01-01 |
description |
Abstract Background Interleukin 15 (IL-15) is a growth and modulating factor for B, T lymphocytes and natural killer cells (NK). Its action on innate and adaptive immunity is modulated by its alpha chain receptor (IL-15Rα). The IL-15/sIL-15Rα complex (IL-15Cx) increases the bioavailability and activity of the cytokine in vivo. IL-15Cx has been used in diseases to dampen IL-15 inflammation by the use of soluble IL-15Ralpha specificity. Here, we aim to evaluate the interest of IL-15Cx in a mouse model of asthma. Methods Using a mouse model of asthma consisting in percutaneous sensitization and intranasal challenge with total house dust mite extract, we evaluated the effect of IL-15Cx injected intraperitoneally four times after a first nasal challenge. Respiratory function was assessed by the technique of forced oscillations (Flexivent®). The effect on bronchial remodeling was evaluated by lung histology. The inflammatory status was analyzed by flow cytometry. Results We observed that the IL-15Cx modulates lung and systemic inflammation by increasing NK cells, CD8+ memory T cells and regulatory cells. However, IL-15Cx displays no effect on bronchial hyperreactivity, bronchial remodeling nor cellular bronchial infiltrate, but limits the secretion of bronchial mucus and modulates only inflammatory response in a HDM-allergic asthma murine model. Conclusions IL-15Cx has a limited effect on immune response in asthma and has no effect on lung function in mice. Thus, it limits its therapeutic potential but might suggest a combinatory potential with other therapeutics. |
topic |
Interleukin-15 Interleukin-15 complex Asthma Allergy |
url |
https://doi.org/10.1186/s12931-020-1301-x |
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